Click here to switch to the map view.

The map label for this gene is clpP

Identifier: 15594956

GI number: 15594956

Start: 637963

End: 638556

Strand: Direct

Name: clpP

Synonym: BB0611

Alternate gene names: 15594956

Gene position: 637963-638556 (Clockwise)

Preceding gene: 15594955

Following gene: 15594957

Centisome position: 70.05

GC content: 33.67

Gene sequence:

>594_bases
ATGGAGTTTATGCATAATTTAATACCCACTGTAATAGAGAATACGGGAAATTATGAGAGAGTATTTGATATATATTCAAG
ATTACTTAGGGAGCGTATAATATTTTTGAGTGGTGAAATTAATGATCCTAAGGCAGATACCGTGATTGCGCAACTTTTGT
TCTTAGAATCAGAAGATTCAAGCAAAGACATTTATCTTTATTTAAATTCTCCAGGCGGCAGTATTACTGCAGGTCTTGCG
ATTTATGATACTATGCAATATATAAAGCCTGATGTAAGGACAATTTGCATTGGGCAAGCCGCTTCAATGGGTGCTTTTTT
ACTTGCTGGTGGCGCTAAAGGTAAAAGAGAATCTCTAACTTATTCTAGAATAATGATTCACCAGCCTTGGGGTGGAATAA
GTGGTCAGGCTAGTGATATTAATATACAAGCTAATGAAATTTTAAGACTTAAAAAATTAATAATAGATATTATGTCTAAT
CAGATAGGAGTTGATAAGGAAAAACTGGCTCTTGATATGGAAAGAGATTATTTTATGACTTCAAGCGATGCTCTTAAATA
TGGTCTTATTGATAGTATCTTAGTAAGGGAGTAG

Upstream 100 bases:

>100_bases
AAAGAGCTAAGAAAAAAATTTTGGAAAATCTTAAAGAGGTAAAGGGTAAAAAACTAAATTTTAGAGATTTTGTTAATTAT
AAAATTTGTGAGTAAGAAAA

Downstream 100 bases:

>100_bases
TTTTATTTTCGGTAAAAGTTTTTATGGCAAGAGTAAAAGGTCAAAAAGTAAAAGAGTGTTCTTTTTGTGGACTTAGTGTC
GCTGAGCTTGGTGGTAATGT

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp 1

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MEFMHNLIPTVIENTGNYERVFDIYSRLLRERIIFLSGEINDPKADTVIAQLLFLESEDSSKDIYLYLNSPGGSITAGLA
IYDTMQYIKPDVRTICIGQAASMGAFLLAGGAKGKRESLTYSRIMIHQPWGGISGQASDINIQANEILRLKKLIIDIMSN
QIGVDKEKLALDMERDYFMTSSDALKYGLIDSILVRE

Sequences:

>Translated_197_residues
MEFMHNLIPTVIENTGNYERVFDIYSRLLRERIIFLSGEINDPKADTVIAQLLFLESEDSSKDIYLYLNSPGGSITAGLA
IYDTMQYIKPDVRTICIGQAASMGAFLLAGGAKGKRESLTYSRIMIHQPWGGISGQASDINIQANEILRLKKLIIDIMSN
QIGVDKEKLALDMERDYFMTSSDALKYGLIDSILVRE
>Mature_197_residues
MEFMHNLIPTVIENTGNYERVFDIYSRLLRERIIFLSGEINDPKADTVIAQLLFLESEDSSKDIYLYLNSPGGSITAGLA
IYDTMQYIKPDVRTICIGQAASMGAFLLAGGAKGKRESLTYSRIMIHQPWGGISGQASDINIQANEILRLKKLIIDIMSN
QIGVDKEKLALDMERDYFMTSSDALKYGLIDSILVRE

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family

Homologues:

Organism=Homo sapiens, GI5174419, Length=189, Percent_Identity=56.0846560846561, Blast_Score=217, Evalue=7e-57,
Organism=Escherichia coli, GI1786641, Length=190, Percent_Identity=60, Blast_Score=245, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17538017, Length=186, Percent_Identity=53.2258064516129, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI20129427, Length=189, Percent_Identity=56.6137566137566, Blast_Score=222, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLPP1_BORBU (O51556)

Other databases:

- EMBL:   AE000783
- PIR:   B70176
- RefSeq:   NP_212745.1
- ProteinModelPortal:   O51556
- SMR:   O51556
- MEROPS:   S14.001
- EnsemblBacteria:   EBBORT00000009146
- GeneID:   1195460
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0611
- NMPDR:   fig|224326.1.peg.995
- TIGR:   BB_0611
- GeneTree:   EBGT00050000007013
- HOGENOM:   HBG558421
- OMA:   SPMEAQD
- ProtClustDB:   PRK00277
- BioCyc:   BBUR224326:BB_0611-MONOMER
- BRENDA:   3.4.21.92
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00444
- InterPro:   IPR001907
- InterPro:   IPR018215
- PANTHER:   PTHR10381
- PRINTS:   PR00127
- TIGRFAMs:   TIGR00493

Pfam domain/function: PF00574 CLP_protease

EC number: =3.4.21.92

Molecular weight: Translated: 22065; Mature: 22065

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00382 CLP_PROTEASE_HIS; PS00381 CLP_PROTEASE_SER

Important sites: ACT_SITE 102-102 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFMHNLIPTVIENTGNYERVFDIYSRLLRERIIFLSGEINDPKADTVIAQLLFLESEDS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCC
SKDIYLYLNSPGGSITAGLAIYDTMQYIKPDVRTICIGQAASMGAFLLAGGAKGKRESLT
CCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCCCCE
YSRIMIHQPWGGISGQASDINIQANEILRLKKLIIDIMSNQIGVDKEKLALDMERDYFMT
EEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCEEEC
SSDALKYGLIDSILVRE
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEFMHNLIPTVIENTGNYERVFDIYSRLLRERIIFLSGEINDPKADTVIAQLLFLESEDS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCC
SKDIYLYLNSPGGSITAGLAIYDTMQYIKPDVRTICIGQAASMGAFLLAGGAKGKRESLT
CCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCCCCE
YSRIMIHQPWGGISGQASDINIQANEILRLKKLIIDIMSNQIGVDKEKLALDMERDYFMT
EEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCEEEC
SSDALKYGLIDSILVRE
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685