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The map label for this gene is murD

Identifier: 15594930

GI number: 15594930

Start: 602374

End: 603729

Strand: Direct

Name: murD

Synonym: BB0585

Alternate gene names: 15594930

Gene position: 602374-603729 (Clockwise)

Preceding gene: 15594929

Following gene: 15594933

Centisome position: 66.14

GC content: 28.69

Gene sequence:

>1356_bases
GTGCTTTTAGACGAAATTAAAAATTTAAATTTTTTAATCATGGGTTTAGGCCTTAATGGAGGAGGAGTAGCTCTTTCTAG
ATTTTTATTAAAGCGTGGGGCAAAATTAGTAATTACTGATCTTAAAAGCGAGACGGAATTAGCTTTAAGTATTGATGCTT
TAAGAGATTTTGAGGATCAAATTAGGTATGTTTTAGGCAAACACGATGTAAACGATTTTAAAAATGCTGACATTGTTGTA
AAAAATCCCGGTGTGAAACCCAATAATAAATATTTAAAGTTTGCAAAACGAATTGAGACAGATATTAGCTTATTTTTAAT
GTTTAACAAGAACCCTATAGTTGCAGTAACAGGCACTAAAGGAAAATCTACTCTTGTATCTCTTTTGTATCAAGCTTTGA
AAGAAAAATATCCGGGGGTAAAGCTTGGAGGCAATATTGGTGTATCTCCTTTAAGTTTTTTTGATCAACTTGATGGAAAA
TCTCCTTTGATTTTAGAGCTCTCTTCTTGGCAGTTGCAATCTTTAGAGAATTTTAATCCTATTATTAGTATTATTACAAA
TGTTTACAACGATCATCAAAACTATTATTTAAATTTTGATGATTATATTATTGATAAGTCAAAAATTTTTGTAAATCAAA
CTTCAGGAATTGTGATTATTCAGGATCAGGCTTATTGCAAATATTTTTCAAAATTTAAATCAAAAGTCAGAGTTATTTTA
TTTTCCGAATTTAATCCTTGTGATTTTGATCAAGATATTTTTTATTGCAATGAAGGCAAAGTATATTTTAATGACAGCTT
GATTGGAAGTTTTTCTAATTCACGAGCTGTTTTTATAATTCCTAAAGTTATTACTTTTTTTGTTTCGTATTATTTAAACA
TAGACCTTAATCGCACGGGTCAGATTTTAAGTAATTTTAAAGGTATTGAGCACAGATTAGAGTTTGTTAAATCAGTTCAA
AATGTAATGTTTTATAATGATACAGCTTCAACTATTCCTGAGTCTACTGTTTTATCTGTTAAAAGTTTGAAAACAAAAGA
TAACCGTATCAACTTAATTGTTGGGGGAACCGATAAAGAGCTTGATTTTTTAAGTTTTAGCAAGATTGCAGATATTGTGA
GAACTTGGATTTTAATAAGAGGTAGTGCAACTGTAAAAATTATTAAGATTTTAGAAAAAAGTAGCATACAATATTTTCTA
TTTGATTCTTTAAGAGATGCAGTAAATTATGCTTTCAAGATTTCAAGTCCGGGTGACATTGTGTTATTTTCTCCTGCTAG
TGCTTCTTTTGAGCTTTTTAATAATGAGTTTGATAGAGGTTTGCAATTTAAAAATTTAGTTAATAATCTTGGTTAA

Upstream 100 bases:

>100_bases
TTGAGGACGCAATCAAACTTGTTGTTTCACTTCCTTATTTTTATAGTGAAAGGTGGATTAAATCCGTTTAAACAGTTGGC
AGGTTTGATATAATTTTATT

Downstream 100 bases:

>100_bases
ATTTTTTTCCTTATTACTTTGTAATAGAAGTATCTAAGTTTTTCAAGATTTGCATAAATCTTATAAATAAAATTTTTGTA
AGTAAAATCATAACAACCAA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLLDEIKNLNFLIMGLGLNGGGVALSRFLLKRGAKLVITDLKSETELALSIDALRDFEDQIRYVLGKHDVNDFKNADIVV
KNPGVKPNNKYLKFAKRIETDISLFLMFNKNPIVAVTGTKGKSTLVSLLYQALKEKYPGVKLGGNIGVSPLSFFDQLDGK
SPLILELSSWQLQSLENFNPIISIITNVYNDHQNYYLNFDDYIIDKSKIFVNQTSGIVIIQDQAYCKYFSKFKSKVRVIL
FSEFNPCDFDQDIFYCNEGKVYFNDSLIGSFSNSRAVFIIPKVITFFVSYYLNIDLNRTGQILSNFKGIEHRLEFVKSVQ
NVMFYNDTASTIPESTVLSVKSLKTKDNRINLIVGGTDKELDFLSFSKIADIVRTWILIRGSATVKIIKILEKSSIQYFL
FDSLRDAVNYAFKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNNLG

Sequences:

>Translated_451_residues
MLLDEIKNLNFLIMGLGLNGGGVALSRFLLKRGAKLVITDLKSETELALSIDALRDFEDQIRYVLGKHDVNDFKNADIVV
KNPGVKPNNKYLKFAKRIETDISLFLMFNKNPIVAVTGTKGKSTLVSLLYQALKEKYPGVKLGGNIGVSPLSFFDQLDGK
SPLILELSSWQLQSLENFNPIISIITNVYNDHQNYYLNFDDYIIDKSKIFVNQTSGIVIIQDQAYCKYFSKFKSKVRVIL
FSEFNPCDFDQDIFYCNEGKVYFNDSLIGSFSNSRAVFIIPKVITFFVSYYLNIDLNRTGQILSNFKGIEHRLEFVKSVQ
NVMFYNDTASTIPESTVLSVKSLKTKDNRINLIVGGTDKELDFLSFSKIADIVRTWILIRGSATVKIIKILEKSSIQYFL
FDSLRDAVNYAFKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNNLG
>Mature_451_residues
MLLDEIKNLNFLIMGLGLNGGGVALSRFLLKRGAKLVITDLKSETELALSIDALRDFEDQIRYVLGKHDVNDFKNADIVV
KNPGVKPNNKYLKFAKRIETDISLFLMFNKNPIVAVTGTKGKSTLVSLLYQALKEKYPGVKLGGNIGVSPLSFFDQLDGK
SPLILELSSWQLQSLENFNPIISIITNVYNDHQNYYLNFDDYIIDKSKIFVNQTSGIVIIQDQAYCKYFSKFKSKVRVIL
FSEFNPCDFDQDIFYCNEGKVYFNDSLIGSFSNSRAVFIIPKVITFFVSYYLNIDLNRTGQILSNFKGIEHRLEFVKSVQ
NVMFYNDTASTIPESTVLSVKSLKTKDNRINLIVGGTDKELDFLSFSKIADIVRTWILIRGSATVKIIKILEKSSIQYFL
FDSLRDAVNYAFKISSPGDIVLFSPASASFELFNNEFDRGLQFKNLVNNLG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=218, Percent_Identity=30.2752293577982, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BORBU (O51532)

Other databases:

- EMBL:   AE000783
- PIR:   H70172
- RefSeq:   NP_212719.1
- ProteinModelPortal:   O51532
- EnsemblBacteria:   EBBORT00000009023
- GeneID:   1195432
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0585
- NMPDR:   fig|224326.1.peg.969
- TIGR:   BB_0585
- GeneTree:   EBGT00050000006940
- HOGENOM:   HBG750024
- OMA:   ASFELFN
- ProtClustDB:   CLSK507682
- BioCyc:   BBUR224326:BB_0585-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51070; Mature: 51070

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDEIKNLNFLIMGLGLNGGGVALSRFLLKRGAKLVITDLKSETELALSIDALRDFEDQ
CCCHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHCHHHH
IRYVLGKHDVNDFKNADIVVKNPGVKPNNKYLKFAKRIETDISLFLMFNKNPIVAVTGTK
HHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLVSLLYQALKEKYPGVKLGGNIGVSPLSFFDQLDGKSPLILELSSWQLQSLENFNP
CHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHCCCCH
IISIITNVYNDHQNYYLNFDDYIIDKSKIFVNQTSGIVIIQDQAYCKYFSKFKSKVRVIL
HHHHHHHHHCCCCCEEEECCCEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCEEEEE
FSEFNPCDFDQDIFYCNEGKVYFNDSLIGSFSNSRAVFIIPKVITFFVSYYLNIDLNRTG
EECCCCCCCCCCEEEECCCEEEECCCEEECCCCCCEEEEHHHHHHHHHHHHEEEEECCCH
QILSNFKGIEHRLEFVKSVQNVMFYNDTASTIPESTVLSVKSLKTKDNRINLIVGGTDKE
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHCCCHHEEEHHHCCCCCCEEEEEECCCCCC
LDFLSFSKIADIVRTWILIRGSATVKIIKILEKSSIQYFLFDSLRDAVNYAFKISSPGDI
CCHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHCCCEEEEHHHHHHHHCEEEEECCCCCE
VLFSPASASFELFNNEFDRGLQFKNLVNNLG
EEECCCCCCHHHHCCHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLLDEIKNLNFLIMGLGLNGGGVALSRFLLKRGAKLVITDLKSETELALSIDALRDFEDQ
CCCHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHCHHHH
IRYVLGKHDVNDFKNADIVVKNPGVKPNNKYLKFAKRIETDISLFLMFNKNPIVAVTGTK
HHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLVSLLYQALKEKYPGVKLGGNIGVSPLSFFDQLDGKSPLILELSSWQLQSLENFNP
CHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHCCCCH
IISIITNVYNDHQNYYLNFDDYIIDKSKIFVNQTSGIVIIQDQAYCKYFSKFKSKVRVIL
HHHHHHHHHCCCCCEEEECCCEEEECCEEEEEECCCEEEEECCHHHHHHHHHHCCEEEEE
FSEFNPCDFDQDIFYCNEGKVYFNDSLIGSFSNSRAVFIIPKVITFFVSYYLNIDLNRTG
EECCCCCCCCCCEEEECCCEEEECCCEEECCCCCCEEEEHHHHHHHHHHHHEEEEECCCH
QILSNFKGIEHRLEFVKSVQNVMFYNDTASTIPESTVLSVKSLKTKDNRINLIVGGTDKE
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHCCCHHEEEHHHCCCCCCEEEEEECCCCCC
LDFLSFSKIADIVRTWILIRGSATVKIIKILEKSSIQYFLFDSLRDAVNYAFKISSPGDI
CCHHHHHHHHHHHHHHEEEECCCCEEEEEHHHHCCCEEEEHHHHHHHHCEEEEECCCCCE
VLFSPASASFELFNNEFDRGLQFKNLVNNLG
EEECCCCCCHHHHCCHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685