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The map label for this gene is crr

Identifier: 15594904

GI number: 15594904

Start: 571283

End: 571852

Strand: Direct

Name: crr

Synonym: BB0559

Alternate gene names: 15594904

Gene position: 571283-571852 (Clockwise)

Preceding gene: 15594903

Following gene: 15594906

Centisome position: 62.73

GC content: 33.16

Gene sequence:

>570_bases
ATGGGGTTTTTAGATTTTTTTAAAAAAACCGCTACATTGGATTTGATTGCTCCGATTAGTGGAAAAGTTATGTCAATTGA
TAAGGTTCCCGATGAAGCTTTTGCTGAAAAAATAGTTGGCGATGGAATTGCAATTCTTCCAACAAGCAATGAGTTGTTGG
CGCCTTGTGATGGGAAAATAGGTAAAATTTTTAAAACCAATCATGCCTTTAGCCTTGAAACTAAAGAGGGCGTTGAAATT
TTTGTCCATTTTGGAATTAATACTCTTAATTTAAATGGTAAGGGTTTTACAAGAGTTGCTGAAGAGGGCATTAATGTTAA
ACAAGGTGAAGTTATTATTAGGCTTGATCTTGAATATTTAAAAGAGCATTCAGAATCCGTTATTACTCCGGTTGTTATTG
CAAATTCTGATGAAGTTTCAAGTATAGAATATTCTTTTGGAAGGCTTGAAAATGATTCTGAATATATTTTATCATCTTCA
ACTGTCTTGACAGAAGAAATTAGGCATAAAATATCTCAAACAAAGCCTGTTATAGCGGGCAAAGATTTGGTGTTGCGAGT
TAAAAAGTAA

Upstream 100 bases:

>100_bases
ATACAATAATGGAATTAGAAGAATTGGCAAATAAGGTTTTAAATAGTGATTCCAAGCAAGAAACTTTAAGTTATTTTGAT
AAATTTATAGGAGATTAGTT

Downstream 100 bases:

>100_bases
AAAGAGAGAGCTTAAAACTCTCTCTTTTTACTTTTAATTATAAGAAGTCTTTTTCTATAAATTCGTTTATTATATTTATA
AATTTTCCTGGATTTTTGCT

Product: PTS system glucose-specific transporter subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEI
FVHFGINTLNLNGKGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENDSEYILSSS
TVLTEEIRHKISQTKPVIAGKDLVLRVKK

Sequences:

>Translated_189_residues
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEI
FVHFGINTLNLNGKGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENDSEYILSSS
TVLTEEIRHKISQTKPVIAGKDLVLRVKK
>Mature_188_residues
GFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEIF
VHFGINTLNLNGKGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENDSEYILSSST
VLTEEIRHKISQTKPVIAGKDLVLRVKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain

Homologues:

Organism=Escherichia coli, GI1788757, Length=135, Percent_Identity=58.5185185185185, Blast_Score=170, Evalue=7e-44,
Organism=Escherichia coli, GI1786894, Length=129, Percent_Identity=51.937984496124, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1790159, Length=128, Percent_Identity=36.71875, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PTGA_BORBU (Q44840)

Other databases:

- EMBL:   U51878
- EMBL:   AE000783
- PIR:   F70169
- RefSeq:   NP_212693.1
- ProteinModelPortal:   Q44840
- SMR:   Q44840
- EnsemblBacteria:   EBBORT00000009183
- GeneID:   1195406
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0559
- NMPDR:   fig|224326.1.peg.943
- TIGR:   BB_0559
- GeneTree:   EBGT00050000006782
- HOGENOM:   HBG517865
- OMA:   RRLISGQ
- PhylomeDB:   Q44840
- ProtClustDB:   PRK09439
- BioCyc:   BBUR224326:BB_0559-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR011055
- InterPro:   IPR001127
- TIGRFAMs:   TIGR00830

Pfam domain/function: PF00358 PTS_EIIA_1; SSF51261 Dup_hybrid_motif

EC number: 2.7.1.69

Molecular weight: Translated: 20781; Mature: 20650

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS51093 PTS_EIIA_TYPE_1; PS00371 PTS_EIIA_TYPE_1_HIS

Important sites: ACT_SITE 83-83

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKI
CCHHHHHHHHCCEEEEECCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCCC
GKIFKTNHAFSLETKEGVEIFVHFGINTLNLNGKGFTRVAEEGINVKQGEVIIRLDLEYL
CEEEECCCEEEEECCCCEEEEEEECEEEEEECCCCHHHHHHCCCCEECCCEEEEEEHHHH
KEHSESVITPVVIANSDEVSSIEYSFGRLENDSEYILSSSTVLTEEIRHKISQTKPVIAG
HCCCCCCEEEEEEECCCCCCEEEEHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEC
KDLVLRVKK
CCEEEEECC
>Mature Secondary Structure 
GFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKI
CHHHHHHHHCCEEEEECCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCCC
GKIFKTNHAFSLETKEGVEIFVHFGINTLNLNGKGFTRVAEEGINVKQGEVIIRLDLEYL
CEEEECCCEEEEECCCCEEEEEEECEEEEEECCCCHHHHHHCCCCEECCCEEEEEEHHHH
KEHSESVITPVVIANSDEVSSIEYSFGRLENDSEYILSSSTVLTEEIRHKISQTKPVIAG
HCCCCCCEEEEEEECCCCCCEEEEHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEC
KDLVLRVKK
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685