Click here to switch to the map view.

The map label for this gene is ptsI

Identifier: 15594903

GI number: 15594903

Start: 569559

End: 571280

Strand: Direct

Name: ptsI

Synonym: BB0558

Alternate gene names: 15594903

Gene position: 569559-571280 (Clockwise)

Preceding gene: 15594902

Following gene: 15594904

Centisome position: 62.54

GC content: 32.93

Gene sequence:

>1722_bases
ATGACTTTATCGGGCAAAAGAATATCCAAAGGGATAGGCATTGGGGAAGTTCTTTGTATTAGGAAAAATTTTGATAAAAT
TATAAGTAGAGAAAAAATAGACTTTTCTCAGGTTGATAGCGAGATATCAAAATTCAATAAAGCGAAGTCAAAAGCAATTG
AAGCGCTTAGGGATCTTGAGAGAAAAGCTATGCTTCAATTTGGAGATGATAAAAAAGGTATTTTTGAAGGTCAGGTGTTG
ATCGTTGAAGACGATGAACTTGATGAGCTTGTTATTGAGCTTATTGTAAAGGAAAATTATAGCGCTGCTTATTCTATTTA
TTTAGCGTTTGAAAATTTGGTTAAAAGTGTAGAAGATTATAAAGATCCTTATTTAAAAGAAAGAGCGTCTGATTATAAGG
ACATTAGAAATAGATTAATTTCTATCATTTTAGGCCAAGTATCCGATTTTTCTGAGATTAATAAAGATATTATTCTTGTT
ACCGAGGAATTAACCCCATCTGATACCATGCAATTTGACTTAAATTATGTTAAAGGGTTTTTAACTGCTGTTGGAGGAGA
AACCTCTCATGCTGCTATTTTAGCAAGAACAATGGGGCTTCCAGCGCTTGTTATGACTTTGTCAGATATTGATGCGTTAA
AGGATGGTGATAAAATAGTAATTGATGCAATGTCTTCTATTGTTATTAAAAATCCTTCTTCTGATGAGATTAATCTTTAT
GAAGGTAAGATTTTGCGACAAGTAGAGTTAGAAAAAGAGCTTTTTTCTTTAAAAGATAAAGATGCTGAAACAAAAGATGG
CACAAAGGTGTTTTTAAAGGCAAATATTGGAACACCTGTTGATATTACCTATGTTAATAAATATGGTGTTGAGGGAATAG
GTCTTTTTAGAACAGAGTTCTTATATATGAGATCTTTACAACCTCCAACAGAAGATGAGCAGTTTGAAACTTATAAGAGA
GTTATAGAAACAATGGAAAAGAAAGGGGTTGTTACGATTCGTACTCTTGATGTTGGTGGTGATAAAGAAATTCCTTATCT
TAATTTTAAGAAAGAGGAAAATCCCTTTTTGGGCTTTCGGGCACTTAGGATGTATAAGGAATATGAGGAATTAATCCAGG
CGCAGTTTAATGCTATTTTTAGGGCCAGTCATTATGGTAAGATAAGGGTAATGGTTCCTATGCTTACCATATATGAAGAG
ATCGAAACGATAGAATATTTTGTTAATAATGCAAAAATCAACTTAAAGTCTAGAGGCTTGCCTTTTGATGAAAATTTGGA
AGTGGGTTGCATGATAGAAGTCCCTTCTGCAGCTTTAATTTCCTCTAAACTTGCCAATAAATTGAAATTTTTTAGCATAG
GGACTAACGATTTAACCCAATATGTTTTAGCTGTTGATCGTGGTAATCAAAAGATATCAAATTTATATGACAAGTATAAT
CCTGCTGTGTTGAAATTAATCAAAAAGGTTCTTGATGATGGGGTTAGTTCTGGAATTGATGTGTCTGTTTGTGGTGAGCT
TGGAGGAGATGATGCTGGAGCACTGCTTCTTGTAGGCCTTGGGTTTAGGTCTTTAAGCATGATTCCTAGTGCTACGCTTA
GAATTAAATATTTGCTTAAAAAGTATACAATAATGGAATTAGAAGAATTGGCAAATAAGGTTTTAAATAGTGATTCCAAG
CAAGAAACTTTAAGTTATTTTGATAAATTTATAGGAGATTAG

Upstream 100 bases:

>100_bases
TTTTGATATGTGCTGAGGGTGAGGATGAAGAGATTGCTGCTTCAGAGCTTGCAGAGCTTATCGAATCTTTTAAGGAATGA
ATTTTAGAAGGATTTAAGTT

Downstream 100 bases:

>100_bases
TTATGGGGTTTTTAGATTTTTTTAAAAAAACCGCTACATTGGATTTGATTGCTCCGATTAGTGGAAAAGTTATGTCAATT
GATAAGGTTCCCGATGAAGC

Product: phosphoenolpyruvate-protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTLSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLERKAMLQFGDDKKGIFEGQVL
IVEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILV
TEELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLY
EGKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEFLYMRSLQPPTEDEQFETYKR
VIETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFRALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEE
IETIEYFVNNAKINLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
PAVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLKKYTIMELEELANKVLNSDSK
QETLSYFDKFIGD

Sequences:

>Translated_573_residues
MTLSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLERKAMLQFGDDKKGIFEGQVL
IVEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILV
TEELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLY
EGKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEFLYMRSLQPPTEDEQFETYKR
VIETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFRALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEE
IETIEYFVNNAKINLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
PAVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLKKYTIMELEELANKVLNSDSK
QETLSYFDKFIGD
>Mature_572_residues
TLSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLERKAMLQFGDDKKGIFEGQVLI
VEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILVT
EELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLYE
GKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEFLYMRSLQPPTEDEQFETYKRV
IETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFRALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEEI
ETIEYFVNNAKINLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYNP
AVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLKKYTIMELEELANKVLNSDSKQ
ETLSYFDKFIGD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=42.5569176882662, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1788726, Length=573, Percent_Identity=32.2862129144852, Blast_Score=295, Evalue=7e-81,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=34.5849802371541, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1789193, Length=480, Percent_Identity=30.625, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1787994, Length=438, Percent_Identity=25.1141552511416, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI226510935, Length=183, Percent_Identity=27.3224043715847, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_BORBU (O51508)

Other databases:

- EMBL:   AE000783
- PIR:   E70169
- RefSeq:   NP_212692.1
- ProteinModelPortal:   O51508
- SMR:   O51508
- EnsemblBacteria:   EBBORT00000008968
- GeneID:   1195405
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0558
- NMPDR:   fig|224326.1.peg.942
- TIGR:   BB_0558
- GeneTree:   EBGT00050000007069
- HOGENOM:   HBG456539
- OMA:   MVEIPST
- PhylomeDB:   O51508
- ProtClustDB:   CLSK507663
- BioCyc:   BBUR224326:BB_0558-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 64596; Mature: 64465

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 190-190 ACT_SITE 504-504 BINDING 297-297 BINDING 334-334 BINDING 433-433 BINDING 454-454 BINDING 455-455 BINDING 456-456 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLE
CCCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKAMLQFGDDKKGIFEGQVLIVEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDY
HHHHHHCCCCCCCEECCEEEEEECCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
KDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILVTEELTPSDTMQFDLNYVKGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEEHHHHHHH
LTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLY
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHEEECCCCCCEEEE
EGKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHH
LYMRSLQPPTEDEQFETYKRVIETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHH
ALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEEIETIEYFVNNAKINLKSRGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
CCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCC
PAVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLK
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHH
KYTIMELEELANKVLNSDSKQETLSYFDKFIGD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLE
CCCCCHHHCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKAMLQFGDDKKGIFEGQVLIVEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDY
HHHHHHCCCCCCCEECCEEEEEECCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
KDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILVTEELTPSDTMQFDLNYVKGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEEHHHHHHH
LTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLY
HHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHEEECCCCCCEEEE
EGKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHH
LYMRSLQPPTEDEQFETYKRVIETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHH
ALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEEIETIEYFVNNAKINLKSRGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
CCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCC
PAVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLK
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHH
KYTIMELEELANKVLNSDSKQETLSYFDKFIGD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685