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The map label for this gene is metK [H]

Identifier: 15594721

GI number: 15594721

Start: 386159

End: 387337

Strand: Direct

Name: metK [H]

Synonym: BB0376

Alternate gene names: 15594721

Gene position: 386159-387337 (Clockwise)

Preceding gene: 15594720

Following gene: 161511080

Centisome position: 42.4

GC content: 33.16

Gene sequence:

>1179_bases
ATGAATAAAATAATAGCAGCGAACCAAACTTTAACTTCTGAGGCTGTATCTGAAGGACATCCAGACAAAATTGCAGATCA
AATCTCTGATGCCATCCTTGATGAAATACTAAAAGAAGATAAAAATGCAAAAGTAGCTTGCGAGGTCATAATTGCACAAA
ATTTAGTAGTAATAGCAGGAGAAATAAATAGTCCTGTAAAAAAAAACATAGATATAAAAGAGGTTGCTAAGAATATCATC
AAAGATATAGGCTATACAAATATTGATTATGGGCTTGATTACAAAACAATAACGGTAATAGACGCTATTGGCAATCAATC
ACGTGACATTATAAACGCAATTGAAAAAAAGGGATCCAATGCCCTTGGAGCAGGGGATCAGGGAATAATATTTGGATATG
CCTGCGATGAAACAAAAAATTTTTTACCTGCTCCTTATGAACTAGCCAATTCAATTCTAAAAAAAGCTAGCAATCTTAGA
AAATCAGGAGCAATAAAATGGTTAAGGCCTGACTCAAAATCTCAAGTTACCATAGAATACGATAAAAACAGAAACCCTGT
AAAAATAAAAAATATTATAGTCTCTCATCAACACCATCCAAACATCTCCCAAAAACTAATACAACAAACAATAATTGAAG
AAATCATCAAGCCCACTGTTCAAGACAAGTCAATGCTTGATGAAAATACTGCTTATTGCATTAATCCTTCTGGAAATTTT
GTAATTGGAGGCCCTACCGGAGACACTGGGCTTACAGGAAGAAAAATTATTGCAGATAGCTATGGAGGATTTGCAAGACA
CGGAGGGGGAGCATACAGCGGAAAAGATGCCACAAAAGTAGATAGATCAGCTGCCTACATGGCAAGATATATTGCAAAAA
ATATGGTGGCAGCCGGAATTTCTAAAGAATTTGAACTACAGCTCGCATATGCAATTGGAATTGAAAACCCAATATCTATT
CAAATAACTGGGGGAATAAATGATCCTAAATATGCAAACAAAATATTAAATTTTATTGTTAATAACTTCGATTTAACTCC
CAACGGTATAATTGAAAAATTAAAACTAAAACAACCCATATATCTTAAAACTTGCACTTATGGTCATTTTGGAAAAAATG
AATTTGAATGGGAAAAATTAGATTTTGTAAAAAAAATACAAACAGCGCTAAAAAAATGA

Upstream 100 bases:

>100_bases
ATCTACCAAACAATAAAGACAACCATATAGACTTTAATAAATTTTTAAAAACATCATCAATAAATTCAAGCAAAATGACA
AAGGAACTTATTAGGCTAAT

Downstream 100 bases:

>100_bases
AAAAAATAACAAGCTTTACAATAGATCATACAAAACTCAACCCTGGCATATATGTCTCAAGAAAAGATACCTTTGAAAAT
GTAATATTTACTACAATAGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNKIIAANQTLTSEAVSEGHPDKIADQISDAILDEILKEDKNAKVACEVIIAQNLVVIAGEINSPVKKNIDIKEVAKNII
KDIGYTNIDYGLDYKTITVIDAIGNQSRDIINAIEKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIKWLRPDSKSQVTIEYDKNRNPVKIKNIIVSHQHHPNISQKLIQQTIIEEIIKPTVQDKSMLDENTAYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITGGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGHFGKNEFEWEKLDFVKKIQTALKK

Sequences:

>Translated_392_residues
MNKIIAANQTLTSEAVSEGHPDKIADQISDAILDEILKEDKNAKVACEVIIAQNLVVIAGEINSPVKKNIDIKEVAKNII
KDIGYTNIDYGLDYKTITVIDAIGNQSRDIINAIEKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIKWLRPDSKSQVTIEYDKNRNPVKIKNIIVSHQHHPNISQKLIQQTIIEEIIKPTVQDKSMLDENTAYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITGGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGHFGKNEFEWEKLDFVKKIQTALKK
>Mature_392_residues
MNKIIAANQTLTSEAVSEGHPDKIADQISDAILDEILKEDKNAKVACEVIIAQNLVVIAGEINSPVKKNIDIKEVAKNII
KDIGYTNIDYGLDYKTITVIDAIGNQSRDIINAIEKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIKWLRPDSKSQVTIEYDKNRNPVKIKNIIVSHQHHPNISQKLIQQTIIEEIIKPTVQDKSMLDENTAYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITGGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGHFGKNEFEWEKLDFVKKIQTALKK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=48.3870967741936, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=47.0430107526882, Blast_Score=342, Evalue=4e-94,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=48.9583333333333, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=46.8911917098446, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=46.9496021220159, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=46.9168900804289, Blast_Score=340, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI25145633, Length=377, Percent_Identity=46.684350132626, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=43.236074270557, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=44.9848024316109, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI25145635, Length=333, Percent_Identity=44.7447447447448, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=45.7236842105263, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=48.780487804878, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=47.4934036939314, Blast_Score=342, Evalue=6e-95,
Organism=Saccharomyces cerevisiae, GI6320710, Length=377, Percent_Identity=47.7453580901857, Blast_Score=339, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=47.6190476190476, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=47.6190476190476, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=47.3544973544974, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=46.4, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24580539, Length=151, Percent_Identity=41.7218543046358, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43071; Mature: 43071

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIAANQTLTSEAVSEGHPDKIADQISDAILDEILKEDKNAKVACEVIIAQNLVVIAG
CCCEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEE
EINSPVKKNIDIKEVAKNIIKDIGYTNIDYGLDYKTITVIDAIGNQSRDIINAIEKKGSN
CCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHCCCC
ALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLRKSGAIKWLRPDSKSQVTIEY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEE
DKNRNPVKIKNIIVSHQHHPNISQKLIQQTIIEEIIKPTVQDKSMLDENTAYCINPSGNF
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCE
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGI
EEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SKEFELQLAYAIGIENPISIQITGGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPI
CCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCE
YLKTCTYGHFGKNEFEWEKLDFVKKIQTALKK
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIIAANQTLTSEAVSEGHPDKIADQISDAILDEILKEDKNAKVACEVIIAQNLVVIAG
CCCEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEE
EINSPVKKNIDIKEVAKNIIKDIGYTNIDYGLDYKTITVIDAIGNQSRDIINAIEKKGSN
CCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHCCCC
ALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLRKSGAIKWLRPDSKSQVTIEY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEE
DKNRNPVKIKNIIVSHQHHPNISQKLIQQTIIEEIIKPTVQDKSMLDENTAYCINPSGNF
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCE
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGI
EEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SKEFELQLAYAIGIENPISIQITGGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPI
CCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCE
YLKTCTYGHFGKNEFEWEKLDFVKKIQTALKK
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA