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The map label for this gene is oppA [H]

Identifier: 15594675

GI number: 15594675

Start: 338358

End: 339983

Strand: Direct

Name: oppA [H]

Synonym: BB0330

Alternate gene names: 15594675

Gene position: 338358-339983 (Clockwise)

Preceding gene: 15594674

Following gene: 15594677

Centisome position: 37.15

GC content: 31.43

Gene sequence:

>1626_bases
ATGAGCTTTAATAAAACTAAAAAAATCGGTAAAAAAATTAAAATAGTAACACTACTTATGCTTGCTGTGTCTTTAATTGC
ATGCAATAATAATTCAGAAAAAGAAAAATTAGCATTTAAAGTATACATAGGGGGAGCGCCCTCATCGCTTGACCCTCATT
TGGTAGATGAGACAATAGGAGCAAGAATTTTAGAACAAATATTCTCAGGGCTTTTGACATTAAATACCAAAACAGGAAAG
CTAAAGCCCGGACTTGCTAAAAATTGGGAAGCCTCAAAAGATAAAAAAACATATCAATTTTATCTAAGGGACAACCTTTT
TTGGAGCGATGGAGTTGAAATTACCGCTGAAGGGATAAGAAAATCTTTTTTAAGAATTTTAAATAAAGAAACAGGATCTA
CAAATGTTGACATGCTCAAATCAATAATAAAAAATGGACAAGAGTATTTTGACGGGAAAGTATCCGATTCTGAACTTGGA
ATCAAGGCAATTGATAGTAAAACGCTGGAAATAACACTTACGGCCCCAAAGCCATATTTTCTTGAACTGCTTCTACATTA
CGCATTCATGCCAGTACCTATTCATGTGATTGAAAAATATAAGGGAAATTGGACAAGCCCTGAAAACATGGTTACTAGCG
GTCCTTTTAAATTAAAAAAAAGATTACCTAATGAAAAAATTATCTTTGAAAAAAACGAACGTTATTATAATGCAAAAGAA
GTAGAACTTGATGAGCTTGTCTACATTACGTCTGACAATGATCTTACTGTGTACAATATGTACAAAAACAACGAAATTGA
TGCTATTTTTAACAGCATCCCGCCGGACATTGTAAATGAAATAAAACTACAAAAAGACTATTACCAACACAAAAGTAATG
CAATTTATTTATATTCATTTAATACAAAAATAAAACCCCTTGATGATGCTAGAGTTAGAGAAGCTTTAACCTTAGCTATT
GACAGAGAAACTTTAACTTACAAAGTGCTAAATGATGGCACAGTTCCTACAAGAGAAATAACTCCTGATCTTAAAAATTA
CAATTACGGTAAAAAATTGGCTTTATTTGATCCTGAAAAATCTAAAAAGCTTTTGGCAGATGCAGGGTATCCTAATGGGA
AAGGATTCCCAATGCTAACACTAAAATATAATACAAACGAAACTCATAAAAAAATTGCTGCATTTATTCAAAACCAATGG
AAAAAAATTCTAAATATCAATCTTATGCTTACCAACGAAAATTGGCCTGTTCTTACCAACAGCAGAAATACTGGCAATTT
TGAAATAATAAGAGTTGGACGCATTGGGGAATATTTAGATCCACACACATACTTTACTATATTCACAAGAGAAAATTCAC
AACTTGCATCATACGGATATTCAAACCTAGAATTTGACAAACTCATCAGAGAATCAGATCTTGAAAAAGATCCTATAAAA
AGAAAACAATTACTCAGAAAAGCAGAATCAATAATAATTGAAAAAGATTTTCCTGCTGCACCAATATACATATATTCTGG
GCATTATCTTTTTAGAAACGATAAATGGACTGGATGGAATCCTAATGTATCAGAGGTTTATTATCTTTCTGAATTAAAAC
CAATTAAAAATGCAAAACATAATTAA

Upstream 100 bases:

>100_bases
GTCTATTATTTATTCCTTATGGACGGTTTTTATTGACTTTATTATAAAAAAAAGATAGTTTAAAACTTATAAATAATTTT
AATTTGGGGGAAAGGTTAAT

Downstream 100 bases:

>100_bases
TTTTAAAAGGATTTTAATATAAATTAAAAACAATATTTAAGTCTAAAAGCAAAGCTGACTAAGCTTAAGACAGTAGCTTT
ATTTAAAACAAAAATACATA

Product: peptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSFNKTKKIGKKIKIVTLLMLAVSLIACNNNSEKEKLAFKVYIGGAPSSLDPHLVDETIGARILEQIFSGLLTLNTKTGK
LKPGLAKNWEASKDKKTYQFYLRDNLFWSDGVEITAEGIRKSFLRILNKETGSTNVDMLKSIIKNGQEYFDGKVSDSELG
IKAIDSKTLEITLTAPKPYFLELLLHYAFMPVPIHVIEKYKGNWTSPENMVTSGPFKLKKRLPNEKIIFEKNERYYNAKE
VELDELVYITSDNDLTVYNMYKNNEIDAIFNSIPPDIVNEIKLQKDYYQHKSNAIYLYSFNTKIKPLDDARVREALTLAI
DRETLTYKVLNDGTVPTREITPDLKNYNYGKKLALFDPEKSKKLLADAGYPNGKGFPMLTLKYNTNETHKKIAAFIQNQW
KKILNINLMLTNENWPVLTNSRNTGNFEIIRVGRIGEYLDPHTYFTIFTRENSQLASYGYSNLEFDKLIRESDLEKDPIK
RKQLLRKAESIIIEKDFPAAPIYIYSGHYLFRNDKWTGWNPNVSEVYYLSELKPIKNAKHN

Sequences:

>Translated_541_residues
MSFNKTKKIGKKIKIVTLLMLAVSLIACNNNSEKEKLAFKVYIGGAPSSLDPHLVDETIGARILEQIFSGLLTLNTKTGK
LKPGLAKNWEASKDKKTYQFYLRDNLFWSDGVEITAEGIRKSFLRILNKETGSTNVDMLKSIIKNGQEYFDGKVSDSELG
IKAIDSKTLEITLTAPKPYFLELLLHYAFMPVPIHVIEKYKGNWTSPENMVTSGPFKLKKRLPNEKIIFEKNERYYNAKE
VELDELVYITSDNDLTVYNMYKNNEIDAIFNSIPPDIVNEIKLQKDYYQHKSNAIYLYSFNTKIKPLDDARVREALTLAI
DRETLTYKVLNDGTVPTREITPDLKNYNYGKKLALFDPEKSKKLLADAGYPNGKGFPMLTLKYNTNETHKKIAAFIQNQW
KKILNINLMLTNENWPVLTNSRNTGNFEIIRVGRIGEYLDPHTYFTIFTRENSQLASYGYSNLEFDKLIRESDLEKDPIK
RKQLLRKAESIIIEKDFPAAPIYIYSGHYLFRNDKWTGWNPNVSEVYYLSELKPIKNAKHN
>Mature_540_residues
SFNKTKKIGKKIKIVTLLMLAVSLIACNNNSEKEKLAFKVYIGGAPSSLDPHLVDETIGARILEQIFSGLLTLNTKTGKL
KPGLAKNWEASKDKKTYQFYLRDNLFWSDGVEITAEGIRKSFLRILNKETGSTNVDMLKSIIKNGQEYFDGKVSDSELGI
KAIDSKTLEITLTAPKPYFLELLLHYAFMPVPIHVIEKYKGNWTSPENMVTSGPFKLKKRLPNEKIIFEKNERYYNAKEV
ELDELVYITSDNDLTVYNMYKNNEIDAIFNSIPPDIVNEIKLQKDYYQHKSNAIYLYSFNTKIKPLDDARVREALTLAID
RETLTYKVLNDGTVPTREITPDLKNYNYGKKLALFDPEKSKKLLADAGYPNGKGFPMLTLKYNTNETHKKIAAFIQNQWK
KILNINLMLTNENWPVLTNSRNTGNFEIIRVGRIGEYLDPHTYFTIFTRENSQLASYGYSNLEFDKLIRESDLEKDPIKR
KQLLRKAESIIIEKDFPAAPIYIYSGHYLFRNDKWTGWNPNVSEVYYLSELKPIKNAKHN

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=512, Percent_Identity=32.8125, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87081878, Length=524, Percent_Identity=33.969465648855, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1789397, Length=474, Percent_Identity=32.2784810126582, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789966, Length=528, Percent_Identity=25.9469696969697, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787052, Length=504, Percent_Identity=24.2063492063492, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789887, Length=493, Percent_Identity=24.7464503042596, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787762, Length=534, Percent_Identity=23.9700374531835, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62368; Mature: 62237

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNKTKKIGKKIKIVTLLMLAVSLIACNNNSEKEKLAFKVYIGGAPSSLDPHLVDETIG
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
ARILEQIFSGLLTLNTKTGKLKPGLAKNWEASKDKKTYQFYLRDNLFWSDGVEITAEGIR
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCEEEHHHHH
KSFLRILNKETGSTNVDMLKSIIKNGQEYFDGKVSDSELGIKAIDSKTLEITLTAPKPYF
HHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCCEEEEEEECCCHHH
LELLLHYAFMPVPIHVIEKYKGNWTSPENMVTSGPFKLKKRLPNEKIIFEKNERYYNAKE
HHHHHHHHHCCCCCEEHHHHCCCCCCCCCCEECCCHHHHHHCCCCEEEEECCCCCCCCCE
VELDELVYITSDNDLTVYNMYKNNEIDAIFNSIPPDIVNEIKLQKDYYQHKSNAIYLYSF
EEEEEEEEEECCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
NTKIKPLDDARVREALTLAIDRETLTYKVLNDGTVPTREITPDLKNYNYGKKLALFDPEK
CCCCCCCCHHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCC
SKKLLADAGYPNGKGFPMLTLKYNTNETHKKIAAFIQNQWKKILNINLMLTNENWPVLTN
CCHHEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEC
SRNTGNFEIIRVGRIGEYLDPHTYFTIFTRENSQLASYGYSNLEFDKLIRESDLEKDPIK
CCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCHHHHHHHCCCCCCHHH
RKQLLRKAESIIIEKDFPAAPIYIYSGHYLFRNDKWTGWNPNVSEVYYLSELKPIKNAKH
HHHHHHHHHHEEEECCCCCCCEEEEECEEEEECCCCCCCCCCHHHEEEEHHCCCCCCCCC
N
C
>Mature Secondary Structure 
SFNKTKKIGKKIKIVTLLMLAVSLIACNNNSEKEKLAFKVYIGGAPSSLDPHLVDETIG
CCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
ARILEQIFSGLLTLNTKTGKLKPGLAKNWEASKDKKTYQFYLRDNLFWSDGVEITAEGIR
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCEEEHHHHH
KSFLRILNKETGSTNVDMLKSIIKNGQEYFDGKVSDSELGIKAIDSKTLEITLTAPKPYF
HHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCCEEEEEEECCCHHH
LELLLHYAFMPVPIHVIEKYKGNWTSPENMVTSGPFKLKKRLPNEKIIFEKNERYYNAKE
HHHHHHHHHCCCCCEEHHHHCCCCCCCCCCEECCCHHHHHHCCCCEEEEECCCCCCCCCE
VELDELVYITSDNDLTVYNMYKNNEIDAIFNSIPPDIVNEIKLQKDYYQHKSNAIYLYSF
EEEEEEEEEECCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
NTKIKPLDDARVREALTLAIDRETLTYKVLNDGTVPTREITPDLKNYNYGKKLALFDPEK
CCCCCCCCHHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCC
SKKLLADAGYPNGKGFPMLTLKYNTNETHKKIAAFIQNQWKKILNINLMLTNENWPVLTN
CCHHEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEC
SRNTGNFEIIRVGRIGEYLDPHTYFTIFTRENSQLASYGYSNLEFDKLIRESDLEKDPIK
CCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCHHHCCCCCCCHHHHHHHCCCCCCHHH
RKQLLRKAESIIIEKDFPAAPIYIYSGHYLFRNDKWTGWNPNVSEVYYLSELKPIKNAKH
HHHHHHHHHHEEEECCCCCCCEEEEECEEEEECCCCCCCCCCHHHEEEEHHCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]