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The map label for this gene is oppA [H]

Identifier: 15594674

GI number: 15594674

Start: 336629

End: 338215

Strand: Direct

Name: oppA [H]

Synonym: BB0329

Alternate gene names: 15594674

Gene position: 336629-338215 (Clockwise)

Preceding gene: 15594673

Following gene: 15594675

Centisome position: 36.96

GC content: 32.14

Gene sequence:

>1587_bases
ATGAAATTACAAAGGTCATTATTTTTAATAATATTTTTTCTAACTTTTCTTTGTTGTAATAACAAGGAAAGAAAAGAAGG
AGTATCATTTAAAATAAGCTTGGGAGCAGAGCCAAGCAGTCTTGACCCTCAATTAGCAGAGGATAATGTCGCATCAAAAA
TGATTGACACAATGTTTAGAGGGATTGTTACAGGAGATCCTAATACAGGGGGAAATAAACCGGGACTTGCAAAAGGGTGG
GATATTTCTTCTGATGGAACAGTTTACACATTTAACCTAAGAGAAAAAATCACTTGGAGTGACGGAGTTGCAATCACTGC
AGAAGGAATTAGAAAATCTTATCTTAGAATTTTAAATAAAGAAACTGGCTCAAAGTACGTTGAAATGGTTAAATCGGTAA
TTAAAAATGGTCAAAAATATTTTGATGGACAAGTGACTGACTCTGAACTTGGAATTAGAGCGATTGATGAAAAAACATTA
GAAATAACACTGGAATCACCAAAACCTTATTTTATTGATATGTTAGTACACCAATCATTTATTCCAGTACCAGTTCATGT
TACCGAAAAGTATGGACAAAACTGGACAAGCCCCGAAAACATGGTGACAAGTGGTCCTTTTAAATTAAAAGAAAGAATTC
CTAACGAAAAATATGTCTTTGAAAAAAATAACAAATACTACGACTCAAATGAAGTAGAATTAGAAGAGATTACATTTTAC
ACAACAAATGACAGCTCAACAGCGTATAAAATGTATGAAAATGAAGAGCTAGATGCAATTTTTGGTTCCATACCCCCAGA
TCTAATCAAAAATCTAAAATTAAGAAGCGACTATTACTCATCAGCTGTTAATGCCATATACTTTTACGCGTTCAATACAC
ACATCAAACCACTTGACAACGTTAAAATTAGAAAAGCCTTAACTCTTGCTATTGACAGAGAAACGCTTACATATAAAGTT
CTTGACAACGGGACTACCCCTACAAGAAGAGCAACTCCCAACTTTAGTTCATATTCTTATGCAAAAAGTTTAGAATTATT
TAATCCTGAAATTGCAAAAACCCTTCTAGCTGAAGCTGGATATCCTAATGGCAATGGATTTCCAATTTTAAAATTAAAAT
ACAATACAAACGAAGCAAATAAAAAAATTTGTGAATTTATTCAAAACCAATGGAAAAAAAATTTAAATATTGATGTGGAA
CTTGAAAACGAAGAATGGACAACATACTTAAACACTAAGGCAAATGGAAATTATGAAATAGCAAGAGCAGGATGGATAGG
CGATTATGCTGATCCTTTGACATTTTTAAGCATATTCACACAAGGATACACACAATTCTCATCTCATAATTACTCAAACC
CAGAATACAACGAACTTATAAAGAAATCCGACCTTGAGCTTGATCCAATAAAAAGACAAGACATTTTAAGACAAGCAGAA
GAGATAATTATTGAAAAAGATTTTCCAATAGCACCAATATACATATATGGGAACAGTTACCTTTTCAGAAATGACAAATG
GACAGGGTGGAACACCAATATTTTAGAAAGATTTGATTTATCTCAGCTAAAATTAAAAAATAAATAA

Upstream 100 bases:

>100_bases
AATTTTAAAATTAAGATTTAAAATTAATATCTACGGAAAGAAAATTATGAAATTAAAGTTAAATTTTAAAACAAACTTTG
AAATGGAGGTATAAAAAATT

Downstream 100 bases:

>100_bases
TATAATAAACTTTATAATATTAAATTTAATTAAATAAAAATCGTCTATTATTTATTCCTTATGGACGGTTTTTATTGACT
TTATTATAAAAAAAAGATAG

Product: peptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKLQRSLFLIIFFLTFLCCNNKERKEGVSFKISLGAEPSSLDPQLAEDNVASKMIDTMFRGIVTGDPNTGGNKPGLAKGW
DISSDGTVYTFNLREKITWSDGVAITAEGIRKSYLRILNKETGSKYVEMVKSVIKNGQKYFDGQVTDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPVPVHVTEKYGQNWTSPENMVTSGPFKLKERIPNEKYVFEKNNKYYDSNEVELEEITFY
TTNDSSTAYKMYENEELDAIFGSIPPDLIKNLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKIRKALTLAIDRETLTYKV
LDNGTTPTRRATPNFSSYSYAKSLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTNEANKKICEFIQNQWKKNLNIDVE
LENEEWTTYLNTKANGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSNPEYNELIKKSDLELDPIKRQDILRQAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGWNTNILERFDLSQLKLKNK

Sequences:

>Translated_528_residues
MKLQRSLFLIIFFLTFLCCNNKERKEGVSFKISLGAEPSSLDPQLAEDNVASKMIDTMFRGIVTGDPNTGGNKPGLAKGW
DISSDGTVYTFNLREKITWSDGVAITAEGIRKSYLRILNKETGSKYVEMVKSVIKNGQKYFDGQVTDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPVPVHVTEKYGQNWTSPENMVTSGPFKLKERIPNEKYVFEKNNKYYDSNEVELEEITFY
TTNDSSTAYKMYENEELDAIFGSIPPDLIKNLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKIRKALTLAIDRETLTYKV
LDNGTTPTRRATPNFSSYSYAKSLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTNEANKKICEFIQNQWKKNLNIDVE
LENEEWTTYLNTKANGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSNPEYNELIKKSDLELDPIKRQDILRQAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGWNTNILERFDLSQLKLKNK
>Mature_528_residues
MKLQRSLFLIIFFLTFLCCNNKERKEGVSFKISLGAEPSSLDPQLAEDNVASKMIDTMFRGIVTGDPNTGGNKPGLAKGW
DISSDGTVYTFNLREKITWSDGVAITAEGIRKSYLRILNKETGSKYVEMVKSVIKNGQKYFDGQVTDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPVPVHVTEKYGQNWTSPENMVTSGPFKLKERIPNEKYVFEKNNKYYDSNEVELEEITFY
TTNDSSTAYKMYENEELDAIFGSIPPDLIKNLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKIRKALTLAIDRETLTYKV
LDNGTTPTRRATPNFSSYSYAKSLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTNEANKKICEFIQNQWKKNLNIDVE
LENEEWTTYLNTKANGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSNPEYNELIKKSDLELDPIKRQDILRQAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGWNTNILERFDLSQLKLKNK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI87081878, Length=478, Percent_Identity=35.5648535564854, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1787495, Length=484, Percent_Identity=33.0578512396694, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1789397, Length=481, Percent_Identity=32.4324324324324, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1789966, Length=454, Percent_Identity=23.7885462555066, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787052, Length=504, Percent_Identity=23.8095238095238, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787762, Length=491, Percent_Identity=23.0142566191446, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789887, Length=465, Percent_Identity=23.4408602150538, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60663; Mature: 60663

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQRSLFLIIFFLTFLCCNNKERKEGVSFKISLGAEPSSLDPQLAEDNVASKMIDTMFR
CCCHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GIVTGDPNTGGNKPGLAKGWDISSDGTVYTFNLREKITWSDGVAITAEGIRKSYLRILNK
CEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCEEEEHHHHHHHHHHHHCC
ETGSKYVEMVKSVIKNGQKYFDGQVTDSELGIRAIDEKTLEITLESPKPYFIDMLVHQSF
CCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEHHHHHCCC
IPVPVHVTEKYGQNWTSPENMVTSGPFKLKERIPNEKYVFEKNNKYYDSNEVELEEITFY
CCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEEEE
TTNDSSTAYKMYENEELDAIFGSIPPDLIKNLKLRSDYYSSAVNAIYFYAFNTHIKPLDN
EECCCCEEEEEECCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCEEEEEEEECCCCCCCC
VKIRKALTLAIDRETLTYKVLDNGTTPTRRATPNFSSYSYAKSLELFNPEIAKTLLAEAG
CEEEEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCC
YPNGNGFPILKLKYNTNEANKKICEFIQNQWKKNLNIDVELENEEWTTYLNTKANGNYEI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEE
ARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSNPEYNELIKKSDLELDPIKRQDILRQAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGWNTNILERFDLSQLKLKNK
HHEEECCCCEEEEEEECCEEEEECCCCCCCCCHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MKLQRSLFLIIFFLTFLCCNNKERKEGVSFKISLGAEPSSLDPQLAEDNVASKMIDTMFR
CCCHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GIVTGDPNTGGNKPGLAKGWDISSDGTVYTFNLREKITWSDGVAITAEGIRKSYLRILNK
CEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCCEEEEHHHHHHHHHHHHCC
ETGSKYVEMVKSVIKNGQKYFDGQVTDSELGIRAIDEKTLEITLESPKPYFIDMLVHQSF
CCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEHHHHHCCC
IPVPVHVTEKYGQNWTSPENMVTSGPFKLKERIPNEKYVFEKNNKYYDSNEVELEEITFY
CCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEEEE
TTNDSSTAYKMYENEELDAIFGSIPPDLIKNLKLRSDYYSSAVNAIYFYAFNTHIKPLDN
EECCCCEEEEEECCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCEEEEEEEECCCCCCCC
VKIRKALTLAIDRETLTYKVLDNGTTPTRRATPNFSSYSYAKSLELFNPEIAKTLLAEAG
CEEEEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCC
YPNGNGFPILKLKYNTNEANKKICEFIQNQWKKNLNIDVELENEEWTTYLNTKANGNYEI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEE
ARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSNPEYNELIKKSDLELDPIKRQDILRQAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGWNTNILERFDLSQLKLKNK
HHEEECCCCEEEEEEECCEEEEECCCCCCCCCHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]