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The map label for this gene is oppA [H]

Identifier: 15594673

GI number: 15594673

Start: 334919

End: 336508

Strand: Direct

Name: oppA [H]

Synonym: BB0328

Alternate gene names: 15594673

Gene position: 334919-336508 (Clockwise)

Preceding gene: 15594669

Following gene: 15594674

Centisome position: 36.78

GC content: 31.01

Gene sequence:

>1590_bases
TTGAAAAAGGAAAATCCCATGAAATATATAAAAATAGCCTTAATGCTAATAATTTTTTCTTTAATAGCATGTATTAGTAA
TGCTAAAAAAGAAAAAATAGTTTTCAGAGTATCAAACTTAAGCGAGCCATCATCACTTGATCCTCAACTCTCAACAGACC
TTTACGGTAGCAACATTATTACAAACCTATTCTTAGGCCTAGCGGTAAAAGATTCTCAAACTGGAAAATATAAACCAGGA
CTTGCAAAAAGTTGGAATATTTCTGAAGATGGAATTATTTACACATTTAACCTAAGAGAAGATATAGTTTGGAGCGATGG
AGTTGCCATTACTGCCGAGGAGATAAAAAAATCATACCTAAGAATTTTAAATAAAAAAACAGCTGCAATGTATGCTAATT
TAATAAAATCTACAATAAAAAATGCACAAGAATATTTCGATGAGACAGTGCCTGAATCTGAGCTTGGCATAAAGGCTATT
GACAGCAAAACCTTAGAGATAACATTAACATCTCCAAAGCCTTATTTTCCTGATATGCTAACACACTCAGCATACATACC
AGTTCCAATGCATATTGTTGAAAAATATGGAGAAAATTGGACAAATCCTGAAAATATAGTTGTTAGTGGCGCATACAAAC
TTAAAGAAAGATCAATTAACGATAAAATCGTAATAGAAAAAAATGAAAAATACTATAATGCAAAAAATGTAGAAATTGAT
GAAGTAATATTTTACCCAACAGAAGGTAGCGTGGCTTACAATATGTACATAAACGGTGAACTCGATTTTCTACAAGGAGC
AGAAAAGAATAATTTAGAAGAAATTAAAATAAGAGATGATTATTATTCTGGGTTAAAAAACGGAATGGCATACATAGCAT
TCAATACAACAATAAAACCACTAGACAATTTAAAAGTTAGACAAGCCATCTCCCTTGCCATTGACAGAGAAACTTTAACT
AAAGTAGTTTTAAAGGGAAGTTCAGATCCAACAAGAAATCTAACTCCAAAATTTGATGATTATTCTTATGGAAAAAATTT
AATACTATTTGATCCTGAGAATGCAAAAAAACTTTTAGCTGAAGCTGGATATCCGGATGGGAAAGGATTCCCCACATTAA
AATATAAAATATCGGAGGGAAGACCAACAACAGCAGAATTTTTGCAAGAACAATTTAAAAAAATACTAAACATTAACTTA
GAAATCGAGAATGAAGAATGGACAACATTCCTAGGAAGCAGAAGAACTGGAAATTACCAAATGTCAAGCGTGGGGTGGAT
AGGAGATTATTTTGATCCCTTAACATTCTTAGACAGCTTATTTACAACAGAAAATCATTTTTTAGGAGCGTACAAATATT
CAAACAAAGAGTATGATGCTTTAATAAAAAAATCTAATTTTGAACTTGATCCAATAAAAAGACAAGACATTTTAAGACAA
GCTGAAGAGATAATAGCAGAAAAAGACTTTCCTATGGCACCTTTATATATACCCAAATCTCATTATCTTTTCAGAAATGA
TAAATGGACAGGGTGGGTACCAAATATCGCAGAAAGCTATTTATATGAAGATATTAAAACTAAAAAATAA

Upstream 100 bases:

>100_bases
TGGAGGCATACATATTTAAAATTAAAACAAAATAAATATTCTTTATTAAAATTTATTAAAAAAGATTAATAAAGAATGAG
AACAAAAAATATATAAAATA

Downstream 100 bases:

>100_bases
TAAAATTAAGTAAAATAAGTAATTTTAAAATTAAGATTTAAAATTAATATCTACGGAAAGAAAATTATGAAATTAAAGTT
AAATTTTAAAACAAACTTTG

Product: peptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKKENPMKYIKIALMLIIFSLIACISNAKKEKIVFRVSNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGKYKPG
LAKSWNISEDGIIYTFNLREDIVWSDGVAITAEEIKKSYLRILNKKTAAMYANLIKSTIKNAQEYFDETVPESELGIKAI
DSKTLEITLTSPKPYFPDMLTHSAYIPVPMHIVEKYGENWTNPENIVVSGAYKLKERSINDKIVIEKNEKYYNAKNVEID
EVIFYPTEGSVAYNMYINGELDFLQGAEKNNLEEIKIRDDYYSGLKNGMAYIAFNTTIKPLDNLKVRQAISLAIDRETLT
KVVLKGSSDPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKISEGRPTTAEFLQEQFKKILNINL
EIENEEWTTFLGSRRTGNYQMSSVGWIGDYFDPLTFLDSLFTTENHFLGAYKYSNKEYDALIKKSNFELDPIKRQDILRQ
AEEIIAEKDFPMAPLYIPKSHYLFRNDKWTGWVPNIAESYLYEDIKTKK

Sequences:

>Translated_529_residues
MKKENPMKYIKIALMLIIFSLIACISNAKKEKIVFRVSNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGKYKPG
LAKSWNISEDGIIYTFNLREDIVWSDGVAITAEEIKKSYLRILNKKTAAMYANLIKSTIKNAQEYFDETVPESELGIKAI
DSKTLEITLTSPKPYFPDMLTHSAYIPVPMHIVEKYGENWTNPENIVVSGAYKLKERSINDKIVIEKNEKYYNAKNVEID
EVIFYPTEGSVAYNMYINGELDFLQGAEKNNLEEIKIRDDYYSGLKNGMAYIAFNTTIKPLDNLKVRQAISLAIDRETLT
KVVLKGSSDPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKISEGRPTTAEFLQEQFKKILNINL
EIENEEWTTFLGSRRTGNYQMSSVGWIGDYFDPLTFLDSLFTTENHFLGAYKYSNKEYDALIKKSNFELDPIKRQDILRQ
AEEIIAEKDFPMAPLYIPKSHYLFRNDKWTGWVPNIAESYLYEDIKTKK
>Mature_529_residues
MKKENPMKYIKIALMLIIFSLIACISNAKKEKIVFRVSNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGKYKPG
LAKSWNISEDGIIYTFNLREDIVWSDGVAITAEEIKKSYLRILNKKTAAMYANLIKSTIKNAQEYFDETVPESELGIKAI
DSKTLEITLTSPKPYFPDMLTHSAYIPVPMHIVEKYGENWTNPENIVVSGAYKLKERSINDKIVIEKNEKYYNAKNVEID
EVIFYPTEGSVAYNMYINGELDFLQGAEKNNLEEIKIRDDYYSGLKNGMAYIAFNTTIKPLDNLKVRQAISLAIDRETLT
KVVLKGSSDPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKISEGRPTTAEFLQEQFKKILNINL
EIENEEWTTFLGSRRTGNYQMSSVGWIGDYFDPLTFLDSLFTTENHFLGAYKYSNKEYDALIKKSNFELDPIKRQDILRQ
AEEIIAEKDFPMAPLYIPKSHYLFRNDKWTGWVPNIAESYLYEDIKTKK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=486, Percent_Identity=33.1275720164609, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87081878, Length=488, Percent_Identity=33.4016393442623, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1789397, Length=479, Percent_Identity=33.4029227557411, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=26.5503875968992, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787052, Length=505, Percent_Identity=27.5247524752475, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789887, Length=457, Percent_Identity=25.382932166302, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=444, Percent_Identity=21.8468468468468, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787762, Length=493, Percent_Identity=22.3123732251521, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60630; Mature: 60630

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKENPMKYIKIALMLIIFSLIACISNAKKEKIVFRVSNLSEPSSLDPQLSTDLYGSNII
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCHHHH
TNLFLGLAVKDSQTGKYKPGLAKSWNISEDGIIYTFNLREDIVWSDGVAITAEEIKKSYL
HHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHH
RILNKKTAAMYANLIKSTIKNAQEYFDETVPESELGIKAIDSKTLEITLTSPKPYFPDML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHH
THSAYIPVPMHIVEKYGENWTNPENIVVSGAYKLKERSINDKIVIEKNEKYYNAKNVEID
CCCCCCCCHHHHHHHHCCCCCCCCCEEEECEEEHHHCCCCCEEEEECCCCEECCCCCEEE
EVIFYPTEGSVAYNMYINGELDFLQGAEKNNLEEIKIRDDYYSGLKNGMAYIAFNTTIKP
EEEEEECCCCEEEEEEECCCCHHHCCCCCCCCEEEEECHHHHHHHHCCEEEEEECCEECC
LDNLKVRQAISLAIDRETLTKVVLKGSSDPTRNLTPKFDDYSYGKNLILFDPENAKKLLA
CCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
EAGYPDGKGFPTLKYKISEGRPTTAEFLQEQFKKILNINLEIENEEWTTFLGSRRTGNYQ
HCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHCCCCCCCEE
MSSVGWIGDYFDPLTFLDSLFTTENHFLGAYKYSNKEYDALIKKSNFELDPIKRQDILRQ
ECCCCCCHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHH
AEEIIAEKDFPMAPLYIPKSHYLFRNDKWTGWVPNIAESYLYEDIKTKK
HHHHHHCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKENPMKYIKIALMLIIFSLIACISNAKKEKIVFRVSNLSEPSSLDPQLSTDLYGSNII
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCHHHH
TNLFLGLAVKDSQTGKYKPGLAKSWNISEDGIIYTFNLREDIVWSDGVAITAEEIKKSYL
HHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCEEEEHHHHHHHHH
RILNKKTAAMYANLIKSTIKNAQEYFDETVPESELGIKAIDSKTLEITLTSPKPYFPDML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHH
THSAYIPVPMHIVEKYGENWTNPENIVVSGAYKLKERSINDKIVIEKNEKYYNAKNVEID
CCCCCCCCHHHHHHHHCCCCCCCCCEEEECEEEHHHCCCCCEEEEECCCCEECCCCCEEE
EVIFYPTEGSVAYNMYINGELDFLQGAEKNNLEEIKIRDDYYSGLKNGMAYIAFNTTIKP
EEEEEECCCCEEEEEEECCCCHHHCCCCCCCCEEEEECHHHHHHHHCCEEEEEECCEECC
LDNLKVRQAISLAIDRETLTKVVLKGSSDPTRNLTPKFDDYSYGKNLILFDPENAKKLLA
CCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
EAGYPDGKGFPTLKYKISEGRPTTAEFLQEQFKKILNINLEIENEEWTTFLGSRRTGNYQ
HCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHCCCCCCCEE
MSSVGWIGDYFDPLTFLDSLFTTENHFLGAYKYSNKEYDALIKKSNFELDPIKRQDILRQ
ECCCCCCHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHH
AEEIIAEKDFPMAPLYIPKSHYLFRNDKWTGWVPNIAESYLYEDIKTKK
HHHHHHCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]