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The map label for this gene is mglA [C]

Identifier: 15594663

GI number: 15594663

Start: 323776

End: 325236

Strand: Reverse

Name: mglA [C]

Synonym: BB0318

Alternate gene names: 15594663

Gene position: 325236-323776 (Counterclockwise)

Preceding gene: 15594664

Following gene: 15594662

Centisome position: 35.71

GC content: 27.1

Gene sequence:

>1461_bases
ATGGTAGAGTTTAAAAACATAGTTAAGTATTTTCCAGATATTGACAAGCCTATTTTGGATAGTATTAATTTAAAAATTGG
GGAAGTTAAAATTTTTACAGTAGTTGGTAAAAATGGAGAAGGAAAGAGCACTCTAGCCAAGATTATTGCCGGACTTATTG
AATTTGATGAGGGTGAAATATTAGTAAATGGCATTAAGCAAAAAAATTGGAATGTAGATAAAGCTAAAAATAATGGTATT
TATCTTGTTTCTCAAGTTCCTAATTTGAAAATGAATTTAAGAGTATGGGAATATTTGAGTATCTATTGGTTTGGTTATGA
ATTTTTCATGCCGATGAATAAATCTAAGACCTACAAATATTATAGATGGCTTATGCAATTTTATAAAATTTCTTTTGATT
TAGATAAGAAAATTAAAGATTTAAATATTAAAGAGATTTATTTTTTACTTATTATTGCTGCTCTTAAAGAGAATGCAAAA
ATAATTATTTTTGATGAGAGTGCTGCTTATTTTTCTCAAAAAGAAGCACAAGCTTTTATAAAATTGCTTGTATTGCTTAA
GAAATCGGGAGTTGCGTCTCTTTTTATTACCCACAGCGAGATTACAGATGCTATAAAATTTAGCGATGAGTTTATTATTT
TAAAAGATGGAAAGTGTTTTAGAACAGTAAACAAAGAATCAATTTTGAGCAAGCTTGAATCCTCTAGTGACAAAGTATTT
GTTGCAAATATTAATTGCAACAAATTTGAAAAAGATCCTATTAAATTTAATTTGTTTTTTGAAGATTTTTGGAAGTATGA
TGTTAGTTTTTCTTTAAATAAAAGGGGTGTTTTAGGGATAATTGGCGAAGAAGCTGTAATTAAAACTTGGGAAAAATTAT
TCTTAGGAGAGCTTCTTTTTGTTGGGTGCATAAAAATTGATGGCATTAGATATGAGCGAATAAATATTTTTGAGTGTAAA
GCGGGATTTTTACCCTTAGGTATTGGTAATTTATTCCCCGATAATAGCAGCATATTAGATAATTTTTTGGCCAAATTTAT
GAATTTTGAAAATAAAATTTTTATTAGGCAATCTTACATTAATCAGATTAAAGATTTTTTTAAAAAAAAAATGGAATTTT
ATAGCGAAGAGAAAATATATAGAATTCTTTATTCAAAATCTTTGGCATTTTCTGGAGGAACTTTGAAGAAATTTGCTCTT
TACAGAGAGATGTATATTGCAAAAAGTTTTTTAATTTGTTTTTCTCCTTTGAGCAATTTAGATCACAAAGCTTATAATGA
AATGTCTGTTGCTATTCGTAATTATTCAAAAGAAAAGCCAGTTCTTTTGATTACTTCCAATTTAGATGAATTGCTTTTGC
TCTCTGATAACATTTTGGCAATGAAAATGGGAGAAGTTTTGTTAAACGTATCTAGAGAAAAGATTAGTAAAGAAAAATTA
AAGGAATTGCTATTTTTATGA

Upstream 100 bases:

>100_bases
TTTATTTAGAGCGAACCAGTCAAAAGTTAAAAGAAGATCTTTTAAAAAAAATAGAAGAGGTTAGTGCAAATGGTATAAAA
ATTAATTTGGAACAAAATTA

Downstream 100 bases:

>100_bases
TCTTTTTTAGAAATAGCTTTATGGCATTAATTTTTTCTTTTTCAATATTAAGTATTAGCTATTTTTTCGGTGATTTTTTT
CAATTTTCTTATATTAAAAT

Product: methylgalactoside ABC transporter ATP-binding protein

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MVEFKNIVKYFPDIDKPILDSINLKIGEVKIFTVVGKNGEGKSTLAKIIAGLIEFDEGEILVNGIKQKNWNVDKAKNNGI
YLVSQVPNLKMNLRVWEYLSIYWFGYEFFMPMNKSKTYKYYRWLMQFYKISFDLDKKIKDLNIKEIYFLLIIAALKENAK
IIIFDESAAYFSQKEAQAFIKLLVLLKKSGVASLFITHSEITDAIKFSDEFIILKDGKCFRTVNKESILSKLESSSDKVF
VANINCNKFEKDPIKFNLFFEDFWKYDVSFSLNKRGVLGIIGEEAVIKTWEKLFLGELLFVGCIKIDGIRYERINIFECK
AGFLPLGIGNLFPDNSSILDNFLAKFMNFENKIFIRQSYINQIKDFFKKKMEFYSEEKIYRILYSKSLAFSGGTLKKFAL
YREMYIAKSFLICFSPLSNLDHKAYNEMSVAIRNYSKEKPVLLITSNLDELLLLSDNILAMKMGEVLLNVSREKISKEKL
KELLFL

Sequences:

>Translated_486_residues
MVEFKNIVKYFPDIDKPILDSINLKIGEVKIFTVVGKNGEGKSTLAKIIAGLIEFDEGEILVNGIKQKNWNVDKAKNNGI
YLVSQVPNLKMNLRVWEYLSIYWFGYEFFMPMNKSKTYKYYRWLMQFYKISFDLDKKIKDLNIKEIYFLLIIAALKENAK
IIIFDESAAYFSQKEAQAFIKLLVLLKKSGVASLFITHSEITDAIKFSDEFIILKDGKCFRTVNKESILSKLESSSDKVF
VANINCNKFEKDPIKFNLFFEDFWKYDVSFSLNKRGVLGIIGEEAVIKTWEKLFLGELLFVGCIKIDGIRYERINIFECK
AGFLPLGIGNLFPDNSSILDNFLAKFMNFENKIFIRQSYINQIKDFFKKKMEFYSEEKIYRILYSKSLAFSGGTLKKFAL
YREMYIAKSFLICFSPLSNLDHKAYNEMSVAIRNYSKEKPVLLITSNLDELLLLSDNILAMKMGEVLLNVSREKISKEKL
KELLFL
>Mature_486_residues
MVEFKNIVKYFPDIDKPILDSINLKIGEVKIFTVVGKNGEGKSTLAKIIAGLIEFDEGEILVNGIKQKNWNVDKAKNNGI
YLVSQVPNLKMNLRVWEYLSIYWFGYEFFMPMNKSKTYKYYRWLMQFYKISFDLDKKIKDLNIKEIYFLLIIAALKENAK
IIIFDESAAYFSQKEAQAFIKLLVLLKKSGVASLFITHSEITDAIKFSDEFIILKDGKCFRTVNKESILSKLESSSDKVF
VANINCNKFEKDPIKFNLFFEDFWKYDVSFSLNKRGVLGIIGEEAVIKTWEKLFLGELLFVGCIKIDGIRYERINIFECK
AGFLPLGIGNLFPDNSSILDNFLAKFMNFENKIFIRQSYINQIKDFFKKKMEFYSEEKIYRILYSKSLAFSGGTLKKFAL
YREMYIAKSFLICFSPLSNLDHKAYNEMSVAIRNYSKEKPVLLITSNLDELLLLSDNILAMKMGEVLLNVSREKISKEKL
KELLFL

Specific function: Part Of The Binding-Protein-Dependent Transport System For Galactosides. Probably Responsible For Energy Coupling To The Transport System. [C]

COG id: COG1129

COG function: function code G; ABC-type sugar transport system, ATPase component

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transporter domains

Homologues:

Organism=Escherichia coli, GI1788472, Length=489, Percent_Identity=25.7668711656442, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1789991, Length=519, Percent_Identity=24.2774566473988, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790190, Length=499, Percent_Identity=23.4468937875752, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787792, Length=501, Percent_Identity=24.3512974051896, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48995001, Length=503, Percent_Identity=24.2544731610338, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788210, Length=500, Percent_Identity=21, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790525, Length=507, Percent_Identity=23.0769230769231, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787143, Length=231, Percent_Identity=29.004329004329, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787029, Length=249, Percent_Identity=23.6947791164659, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y318_BORBU (Q44848)

Other databases:

- EMBL:   AE000783
- EMBL:   L31422
- PIR:   E70139
- RefSeq:   NP_212452.1
- ProteinModelPortal:   Q44848
- SMR:   Q44848
- EnsemblBacteria:   EBBORT00000008773
- GeneID:   1195155
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0318
- NMPDR:   fig|224326.1.peg.702
- TIGR:   BB_0318
- GeneTree:   EBGT00050000006897
- HOGENOM:   HBG354415
- OMA:   WEYLSIY
- ProtClustDB:   CLSK507514
- BioCyc:   BBUR224326:BB_0318-MONOMER
- GO:   GO:0006810
- InterPro:   IPR003439
- InterPro:   IPR003593
- SMART:   SM00382

Pfam domain/function: PF00005 ABC_tran

EC number: NA

Molecular weight: Translated: 56532; Mature: 56532

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00211 ABC_TRANSPORTER_1; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFKNIVKYFPDIDKPILDSINLKIGEVKIFTVVGKNGEGKSTLAKIIAGLIEFDEGEI
CCCHHHHHHHCCCCCCHHHHCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
LVNGIKQKNWNVDKAKNNGIYLVSQVPNLKMNLRVWEYLSIYWFGYEFFMPMNKSKTYKY
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHEEEEEEEEEEEEEECCCCCHHHH
YRWLMQFYKISFDLDKKIKDLNIKEIYFLLIIAALKENAKIIIFDESAAYFSQKEAQAFI
HHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
KLLVLLKKSGVASLFITHSEITDAIKFSDEFIILKDGKCFRTVNKESILSKLESSSDKVF
HHHHHHHCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCEEECCCHHHHHHHHHCCCCEEE
VANINCNKFEKDPIKFNLFFEDFWKYDVSFSLNKRGVLGIIGEEAVIKTWEKLFLGELLF
EEECCCCCCCCCCEEEEEEEEHHCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHH
VGCIKIDGIRYERINIFECKAGFLPLGIGNLFPDNSSILDNFLAKFMNFENKIFIRQSYI
HHEEEECCEEEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHH
NQIKDFFKKKMEFYSEEKIYRILYSKSLAFSGGTLKKFALYREMYIAKSFLICFSPLSNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHCC
DHKAYNEMSVAIRNYSKEKPVLLITSNLDELLLLSDNILAMKMGEVLLNVSREKISKEKL
CHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
KELLFL
HHHHCC
>Mature Secondary Structure
MVEFKNIVKYFPDIDKPILDSINLKIGEVKIFTVVGKNGEGKSTLAKIIAGLIEFDEGEI
CCCHHHHHHHCCCCCCHHHHCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
LVNGIKQKNWNVDKAKNNGIYLVSQVPNLKMNLRVWEYLSIYWFGYEFFMPMNKSKTYKY
EEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEHHHEEEEEEEEEEEEEECCCCCHHHH
YRWLMQFYKISFDLDKKIKDLNIKEIYFLLIIAALKENAKIIIFDESAAYFSQKEAQAFI
HHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
KLLVLLKKSGVASLFITHSEITDAIKFSDEFIILKDGKCFRTVNKESILSKLESSSDKVF
HHHHHHHCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCEEECCCHHHHHHHHHCCCCEEE
VANINCNKFEKDPIKFNLFFEDFWKYDVSFSLNKRGVLGIIGEEAVIKTWEKLFLGELLF
EEECCCCCCCCCCEEEEEEEEHHCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHH
VGCIKIDGIRYERINIFECKAGFLPLGIGNLFPDNSSILDNFLAKFMNFENKIFIRQSYI
HHEEEECCEEEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHH
NQIKDFFKKKMEFYSEEKIYRILYSKSLAFSGGTLKKFALYREMYIAKSFLICFSPLSNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHCC
DHKAYNEMSVAIRNYSKEKPVLLITSNLDELLLLSDNILAMKMGEVLLNVSREKISKEKL
CHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
KELLFL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685