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The map label for this gene is pucM

Identifier: 255767744

GI number: 255767744

Start: 3334646

End: 3334990

Strand: Direct

Name: pucM

Synonym: BSU32460

Alternate gene names: 255767744

Gene position: 3334646-3334990 (Clockwise)

Preceding gene: 16080298

Following gene: 255767745

Centisome position: 79.1

GC content: 46.38

Gene sequence:

>345_bases
ATGGGAAAACTGACAACCCATATTTTAGATTTAACCTGCGGCAAACCAGCGGCGAACGTAAAAATTGGATTGAAGCGGCT
GGGCGAAAGTATTATGAAAGAGGTATACACTAACAATGACGGCAGGGTCGATGTCCCTCTGCTGGCTGGTGAAGAGCTGA
TGTCAGGAGAGTATGTGATGGAATTTCATGCAGGAGACTATTTTGCGAGTAAAAACATGAACGCAGCCGATCAGCCGTTT
TTAACCATCGTCACCGTCCGTTTTCAGCTAGCAGATCCAGATGCACATTATCATATCCCGCTCTTGCTGTCGCCGTTTGG
TTATCAGGTGTATAGGGGGAGTTAA

Upstream 100 bases:

>100_bases
CGCGCCCGCCATACGGTTTCCAACATTTTACCGTGACAAGAGAAGACGCCGAGAAAGAAAAACAGAAAGCCGCTGAAAAA
TGTCGGAGCCTGAAAGCCTG

Downstream 100 bases:

>100_bases
GAAGGAAGCCCGCCTCACCGGCGGGCTTCTTTTTGCACTTCAATTAACTTTTGATCCTAGGACAGTACTTGAAGTGATCC
CCACACTTAGTGAAACAACA

Product: urate oxidase structural factor

Products: NA

Alternate protein names: HIU hydrolase; HIUHase; Transthyretin-related protein; TRP

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MGKLTTHILDLTCGKPAANVKIGLKRLGESIMKEVYTNNDGRVDVPLLAGEELMSGEYVMEFHAGDYFASKNMNAADQPF
LTIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS

Sequences:

>Translated_114_residues
MGKLTTHILDLTCGKPAANVKIGLKRLGESIMKEVYTNNDGRVDVPLLAGEELMSGEYVMEFHAGDYFASKNMNAADQPF
LTIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS
>Mature_113_residues
GKLTTHILDLTCGKPAANVKIGLKRLGESIMKEVYTNNDGRVDVPLLAGEELMSGEYVMEFHAGDYFASKNMNAADQPFL
TIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS

Specific function: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU)

COG id: COG2351

COG function: function code R; Transthyretin-like protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily

Homologues:

Organism=Escherichia coli, GI1788281, Length=114, Percent_Identity=35.9649122807018, Blast_Score=79, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI115534555, Length=116, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532922, Length=121, Percent_Identity=32.2314049586777, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532920, Length=121, Percent_Identity=32.2314049586777, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652571, Length=116, Percent_Identity=38.7931034482759, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIUH_BACSU (O32142)

Other databases:

- EMBL:   AL009126
- PIR:   H70016
- RefSeq:   NP_391126.2
- PDB:   2H0E
- PDB:   2H0F
- PDB:   2H0J
- PDBsum:   2H0E
- PDBsum:   2H0F
- PDBsum:   2H0J
- ProteinModelPortal:   O32142
- SMR:   O32142
- EnsemblBacteria:   EBBACT00000002213
- GeneID:   937977
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32460
- NMPDR:   fig|224308.1.peg.3252
- GenoList:   BSU32460
- GeneTree:   EBGT00050000006214
- HOGENOM:   HBG445217
- ProtClustDB:   CLSK2816785
- BioCyc:   BSUB:BSU32460-MONOMER
- GO:   GO:0006810
- InterPro:   IPR014306
- InterPro:   IPR000895
- Gene3D:   G3DSA:2.60.40.180
- PRINTS:   PR00189
- TIGRFAMs:   TIGR02962

Pfam domain/function: PF00576 Transthyretin; SSF49472 Transthyretin

EC number: =3.5.2.17

Molecular weight: Translated: 12635; Mature: 12503

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00768 TRANSTHYRETIN_1; PS00769 TRANSTHYRETIN_2

Important sites: BINDING 7-7 BINDING 42-42 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLTTHILDLTCGKPAANVKIGLKRLGESIMKEVYTNNDGRVDVPLLAGEELMSGEYVM
CCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEE
EFHAGDYFASKNMNAADQPFLTIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS
EEECCCEEECCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCEEEEECC
>Mature Secondary Structure 
GKLTTHILDLTCGKPAANVKIGLKRLGESIMKEVYTNNDGRVDVPLLAGEELMSGEYVM
CCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEE
EFHAGDYFASKNMNAADQPFLTIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS
EEECCCEEECCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136