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The map label for this gene is pucL

Identifier: 16080298

GI number: 16080298

Start: 3333162

End: 3334646

Strand: Direct

Name: pucL

Synonym: BSU32450

Alternate gene names: 16080298

Gene position: 3333162-3334646 (Clockwise)

Preceding gene: 16080297

Following gene: 255767744

Centisome position: 79.07

GC content: 47.95

Gene sequence:

>1485_bases
ATGTTCACAATGGATGACCTGAACCAAATGGACACACAAACACTGACAGACACACTTGGCTCTATTTTTGAACACTCTTC
ATGGATTGCGGAGAGATCCGCAGCACTACGGCCGTTTTCGTCCCTTTCTGATCTTCACCGCAAAATGACTGGCATTGTAA
AAGCTGCGGATCGCGAGACACAGCTTGATTTAATCAAAAAGCATCCCCGGCTCGGAACAAAGAAAACAATGAGCGATGAC
TCGGTACGAGAGCAGCAGAACGCAGGGCTCGGCAAATTAGAACAGCAGGAATACGAGGAGTTTCTCATGCTGAATGAACA
CTATTATGATCGCTTCGGCTTTCCTTTTATTTTAGCGGTGAAGGGAAAGACGAAACAGGACATTCACCAAGCCCTGCTGG
CAAGGCTTGAGAGCGAACGAGAAACGGAGTTCCAGCAGGCCCTTATAGAAATTTACCGAATCGCCCGCTTTCGTCTGGCT
GACATCATCACTGAAAAAGGAGAGACGCAAATGAAAAGAACCATGTCTTATGGCAAAGGAAATGTATTTGCATACCGAAC
GTATTTAAAACCGCTCACTGGAGTGAAGCAAATCCCGGAATCATCTTTTGCCGGAAGAGATAATACCGTTGTCGGCGTTG
ATGTGACATGCGAAATTGGCGGAGAAGCCTTCCTGCCATCATTTACAGACGGAGACAACACTCTCGTCGTGGCAACGGAT
TCAATGAAAAACTTTATCCAGCGCCATCTCGCATCCTATGAAGGAACGACAACCGAGGGGTTCCTACACTATGTAGCTCA
CCGATTTTTAGATACCTATTCTCATATGGACACGATCACTCTGACTGGCGAAGACATTCCGTTTGAAGCAATGCCTGCAT
ACGAGGAGAAAGAGCTCAGCACAAGCCGCCTCGTCTTCAGAAGATCGCGTAATGAACGAAGCCGCTCTGTGCTGAAAGCA
GAACGAAGCGGGAATACCATAACGATTACAGAGCAATACAGCGAAATCATGGATCTTCAGCTCGTCAAGGTGAGCGGCAA
CTCCTTCGTCGGCTTCATCCGGGACGAATATACGACTCTCCCGGAAGACGGCAACCGCCCGCTGTTTGTCTATTTAAACA
TCAGCTGGCAATATGAAAATACAAATGACTCATACGCTTCTGATCCAGCACGCTACGTTGCGGCTGAACAAGTCCGCGAC
TTAGCGAGCACCGTTTTTCACGAGCTCGAAACCCCTTCAATCCAAAACCTAATCTATCATATCGGCTGCAGAATATTAGC
GAGGTTCCCGCAGCTCACTGATGTCAGCTTCCAATCTCAAAATCACACGTGGGATACGGTTGTCGAAGAAATCCCGGGCT
CAAAAGGAAAAGTCTACACCGAACCGCGCCCGCCATACGGTTTCCAACATTTTACCGTGACAAGAGAAGACGCCGAGAAA
GAAAAACAGAAAGCCGCTGAAAAATGTCGGAGCCTGAAAGCCTGA

Upstream 100 bases:

>100_bases
CGGAATTGTGATCGGCAGTTTGACGGCCATTGCACTGCACGCTTTTTTTCAAACCAAACAGCCGAATAGCGCAGACATAA
AAACGTAAAGGAAGATGCCA

Downstream 100 bases:

>100_bases
TGGGAAAACTGACAACCCATATTTTAGATTTAACCTGCGGCAAACCAGCGGCGAACGTAAAAATTGGATTGAAGCGGCTG
GGCGAAAGTATTATGAAAGA

Product: urate oxidase with peroxide reductase N-terminal domain

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFTMDDLNQMDTQTLTDTLGSIFEHSSWIAERSAALRPFSSLSDLHRKMTGIVKAADRETQLDLIKKHPRLGTKKTMSDD
SVREQQNAGLGKLEQQEYEEFLMLNEHYYDRFGFPFILAVKGKTKQDIHQALLARLESERETEFQQALIEIYRIARFRLA
DIITEKGETQMKRTMSYGKGNVFAYRTYLKPLTGVKQIPESSFAGRDNTVVGVDVTCEIGGEAFLPSFTDGDNTLVVATD
SMKNFIQRHLASYEGTTTEGFLHYVAHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKELSTSRLVFRRSRNERSRSVLKA
ERSGNTITITEQYSEIMDLQLVKVSGNSFVGFIRDEYTTLPEDGNRPLFVYLNISWQYENTNDSYASDPARYVAAEQVRD
LASTVFHELETPSIQNLIYHIGCRILARFPQLTDVSFQSQNHTWDTVVEEIPGSKGKVYTEPRPPYGFQHFTVTREDAEK
EKQKAAEKCRSLKA

Sequences:

>Translated_494_residues
MFTMDDLNQMDTQTLTDTLGSIFEHSSWIAERSAALRPFSSLSDLHRKMTGIVKAADRETQLDLIKKHPRLGTKKTMSDD
SVREQQNAGLGKLEQQEYEEFLMLNEHYYDRFGFPFILAVKGKTKQDIHQALLARLESERETEFQQALIEIYRIARFRLA
DIITEKGETQMKRTMSYGKGNVFAYRTYLKPLTGVKQIPESSFAGRDNTVVGVDVTCEIGGEAFLPSFTDGDNTLVVATD
SMKNFIQRHLASYEGTTTEGFLHYVAHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKELSTSRLVFRRSRNERSRSVLKA
ERSGNTITITEQYSEIMDLQLVKVSGNSFVGFIRDEYTTLPEDGNRPLFVYLNISWQYENTNDSYASDPARYVAAEQVRD
LASTVFHELETPSIQNLIYHIGCRILARFPQLTDVSFQSQNHTWDTVVEEIPGSKGKVYTEPRPPYGFQHFTVTREDAEK
EKQKAAEKCRSLKA
>Mature_494_residues
MFTMDDLNQMDTQTLTDTLGSIFEHSSWIAERSAALRPFSSLSDLHRKMTGIVKAADRETQLDLIKKHPRLGTKKTMSDD
SVREQQNAGLGKLEQQEYEEFLMLNEHYYDRFGFPFILAVKGKTKQDIHQALLARLESERETEFQQALIEIYRIARFRLA
DIITEKGETQMKRTMSYGKGNVFAYRTYLKPLTGVKQIPESSFAGRDNTVVGVDVTCEIGGEAFLPSFTDGDNTLVVATD
SMKNFIQRHLASYEGTTTEGFLHYVAHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKELSTSRLVFRRSRNERSRSVLKA
ERSGNTITITEQYSEIMDLQLVKVSGNSFVGFIRDEYTTLPEDGNRPLFVYLNISWQYENTNDSYASDPARYVAAEQVRD
LASTVFHELETPSIQNLIYHIGCRILARFPQLTDVSFQSQNHTWDTVVEEIPGSKGKVYTEPRPPYGFQHFTVTREDAEK
EKQKAAEKCRSLKA

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin

COG id: COG3648

COG function: function code Q; Uricase (urate oxidase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family

Homologues:

Organism=Homo sapiens, GI157743280, Length=164, Percent_Identity=32.3170731707317, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCL_BACSU (O32141)

Other databases:

- EMBL:   AL009126
- PIR:   G70016
- RefSeq:   NP_391125.1
- ProteinModelPortal:   O32141
- EnsemblBacteria:   EBBACT00000000329
- GeneID:   936669
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32450
- NMPDR:   fig|224308.1.peg.3251
- GenoList:   BSU32450
- GeneTree:   EBGT00050000006115
- OMA:   YTEPRPP
- ProtClustDB:   CLSK552104
- BioCyc:   BSUB:BSU32450-MONOMER
- BRENDA:   1.7.3.3
- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842
- Gene3D:   G3DSA:3.10.270.10
- PRINTS:   PR00093
- TIGRFAMs:   TIGR03164
- TIGRFAMs:   TIGR03383

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase

EC number: =1.7.3.3

Molecular weight: Translated: 56560; Mature: 56560

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00366 URICASE

Important sites: ACT_SITE 68-68 ACT_SITE 366-366 ACT_SITE 415-415 BINDING 69-69 BINDING 239-239 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTMDDLNQMDTQTLTDTLGSIFEHSSWIAERSAALRPFSSLSDLHRKMTGIVKAADRET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
QLDLIKKHPRLGTKKTMSDDSVREQQNAGLGKLEQQEYEEFLMLNEHYYDRFGFPFILAV
HHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
KGKTKQDIHQALLARLESERETEFQQALIEIYRIARFRLADIITEKGETQMKRTMSYGKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
NVFAYRTYLKPLTGVKQIPESSFAGRDNTVVGVDVTCEIGGEAFLPSFTDGDNTLVVATD
CEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEH
SMKNFIQRHLASYEGTTTEGFLHYVAHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKELS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHH
TSRLVFRRSRNERSRSVLKAERSGNTITITEQYSEIMDLQLVKVSGNSFVGFIRDEYTTL
HHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHHHHCEEEEEECCCCEEEEECCCCCCC
PEDGNRPLFVYLNISWQYENTNDSYASDPARYVAAEQVRDLASTVFHELETPSIQNLIYH
CCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGCRILARFPQLTDVSFQSQNHTWDTVVEEIPGSKGKVYTEPRPPYGFQHFTVTREDAEK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEHHHHHH
EKQKAAEKCRSLKA
HHHHHHHHHHCCCC
>Mature Secondary Structure
MFTMDDLNQMDTQTLTDTLGSIFEHSSWIAERSAALRPFSSLSDLHRKMTGIVKAADRET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
QLDLIKKHPRLGTKKTMSDDSVREQQNAGLGKLEQQEYEEFLMLNEHYYDRFGFPFILAV
HHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
KGKTKQDIHQALLARLESERETEFQQALIEIYRIARFRLADIITEKGETQMKRTMSYGKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
NVFAYRTYLKPLTGVKQIPESSFAGRDNTVVGVDVTCEIGGEAFLPSFTDGDNTLVVATD
CEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEH
SMKNFIQRHLASYEGTTTEGFLHYVAHRFLDTYSHMDTITLTGEDIPFEAMPAYEEKELS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHH
TSRLVFRRSRNERSRSVLKAERSGNTITITEQYSEIMDLQLVKVSGNSFVGFIRDEYTTL
HHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHHHHHHHCEEEEEECCCCEEEEECCCCCCC
PEDGNRPLFVYLNISWQYENTNDSYASDPARYVAAEQVRDLASTVFHELETPSIQNLIYH
CCCCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGCRILARFPQLTDVSFQSQNHTWDTVVEEIPGSKGKVYTEPRPPYGFQHFTVTREDAEK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEHHHHHH
EKQKAAEKCRSLKA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136