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The map label for this gene is mrpA

Identifier: 161511068

GI number: 161511068

Start: 3246598

End: 3249003

Strand: Direct

Name: mrpA

Synonym: BSU31600

Alternate gene names: 161511068

Gene position: 3246598-3249003 (Clockwise)

Preceding gene: 16080210

Following gene: 16080213

Centisome position: 77.01

GC content: 44.68

Gene sequence:

>2406_bases
TTGCAGCTCTTACATCTTGCTATTTTATCGCCTTTTTTATTTGCTTTTATCATTCCCTTCTTGGCAAAATACGCAAAAAG
AGTGCATACGGGCTGGTTTGTGTTGATCCTGCCCGTCTTGCTGTTTATATACTTTCTCCCAATGATCAGGATGACGCAAT
CTGGAGAAACACTTCGATCGGTATTAGAGTGGATTCCGTCACTCGGCATTAACTTTACCGTTTATATAGACGGCCTCGGG
TTATTATTCGCTTTACTGATCACAGGCATTGGCTCTTTGGTCACTCTTTACAGCATTTTTTATTTATCGAAAGAAAAAGA
ACAGCTTGGGCCCTTTTATGTCTACTTATTGATGTTCATGGGCGCCATGCTCGGCGTCGTTCTAGTAGACAATGTCATGG
TTCTCTACATGTTCTGGGAGCTTACAAGCCTTTCATCCTTTTTGCTGATCGGCTATTGGTATAAGCGGGAAAAGTCGCGC
TATGGCGCAGCCAAATCACTTTTGATTACGGTCAGCGGCGGGCTGTGCATGCTCGGCGGATTTATTCTCCTTTATTTGAT
AACGGATTCCTTCAGTATTCGAGAGATGGTTCACCAGGTTCAATTAATTGCCGGCCACGAATTGTTTATCCCGGCTATGA
TTCTCATTTTATTAGGGGCTTTTACGAAATCGGCGCAATTTCCTTTTTACATCTGGCTCCCTGACGCGATGGAAGCTCCG
ACGCCTGTCAGCGCTTATCTCCATTCAGCTACTATGGTAAAAGCCGGCATTTATGTGATTGCGAGATTCAGTCCTATTTT
CGCCTTTTCAGCTCAGTGGTTTTGGATCGTGTCCCTCGTCGGCCTCTTTACGATGGTTTGGGGTTCATTCCATGCCGTGA
AACAAACTGATTTAAAATCGATTTTGGCGTTTTCAACCGTCAGCCAGCTCGGCATGATCATCTCCATGCTCGGAGTCAGT
GCTGCGGCCCTTCATTACGGACATACAGAATATTATACAGTTGCCGCTATGGCTGCCATTTTCCATTTGATCAACCACGC
AACCTTTAAAGGAAGCTTGTTTATGGCAGTCGGGATTATCGATCACGAAACAGGCACACGGGATATCCGGAAGCTAGGCG
GCCTGATGGCGATTATGCCGATTACTTTTACCATTTCCTTAATCGGGACATTTTCAATGGCCGGCCTACCGCCGTTTAAC
GGATTTTTGAGTAAAGAAATGTTTTTCACAAGCATGCTTCGCGTGACACATTTTGACCTGTTCAACGTGCAGACATGGGG
CGTTCTGTTCCCTCTTTTCGCCTGGATCGGAAGTGTATTTACGTTTATCTACAGCATGAAATTGCTGTTTAAAACATTCA
GAGGAAATTACCAGCCTGAACAGCTTGAAAAACAGGCTCACGAAGCGCCTGTCGGTATGCTCGTTCCACCGGTCATTCTT
GTCGCTTTGGCTGTCAGTCTCTTCTTTTTCCCGAATATATTGTCGTACAGCCTGATAGAGCCTGCGATGAACTCAATCTA
TCCGACACTGCTTGACGGCCATGAGAAATTCCACGTTCACATTTCACAGTGGCATGGGGTTACAACGGAATTGCTCATGA
CTGCGGGTATTGTTGTGATCGGAACAATTGGTTATTTGTCGCTGAATAAGTGGAAAGGAATCTATAAACTGTTTCCTTCG
AAACTCACACTCAACCGGTTATACGATAAGCTTTTGACTATGATGGAGAAAGGCTCTTACCGGGTCACAAAACAATATAT
GACCGGGTTTTTAAGAGATTATTTGCTGTATATCTTCGCTGGTTTCATCATTCTGATAGGCGGAGCTTTTGCCATTAAAG
GCGGATTTTCATTTAAAACGGAAGGCATGGCTAAAATCGGTGTATACGAAATCATTTTGACGCTTGTCATGATCAGCGCC
ACAGTGGCGACTGTTTTTGCTAGGTCAAGACTGACGGCGATCATTGCACTCGGTGTTGTCGGTTATACGCTTGCATTGTT
TTTTGTCATTTTCAGAGCGCCGGACTTGGCATTGACTCAGCTCGTCATTGAAACGATTTCTGTGGCACTGTTTCTGCTTT
GCTTCTACCATTTGCCGAAACTGAGGCTGAAAACAAAAACGAGAACGTTCCGGATGACGAATTTTATCATTTCTTTAGGC
GTCGGTGTTATCGTTACACTGCTCGGCATCGCATCCTCAAGCCAGCGGACAAAAGACAGCATTGCGTCCTTCTTTGTCAA
ACACAGCCACGATCTCGGCGGAGGGGATAATGTCGTCAATGTCATTCTCGTTGATTTCAGGGGATTTGATACAATGTTTG
AAATTACGGTATTAACGATAGCCGCTCTCGGCATTTACAGCATGATTAAAACAAAAGTAAAAGAGGAGGGGAAAAGCGGT
GAATGA

Upstream 100 bases:

>100_bases
CTTGCAGAAAAGTGCGATATAATTTGGACTAGGTCGTTTGACCGTATATTTTATACATAGCGCATTTTTCAATTGAGAAC
GAGAAAAGGAGGTCTTATCT

Downstream 100 bases:

>100_bases
ACAAAAAACAAATGACTTGATTCTTCAAACCGCGACAAAGCTTGTATCCTTTATCATTTTGCTTTTCTCTTTCTATTTAT
TTTTATCAGGGCATAACGCG

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRSVLEWIPSLGINFTVYIDGLG
LLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFMGAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSR
YGAAKSLLITVSGGLCMLGGFILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAP
TPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKSILAFSTVSQLGMIISMLGVS
AAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFN
GFLSKEMFFTSMLRVTHFDLFNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVIL
VALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVIGTIGYLSLNKWKGIYKLFPS
KLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFAGFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISA
TVATVFARSRLTAIIALGVVGYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLG
VGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTIAALGIYSMIKTKVKEEGKSG
E

Sequences:

>Translated_801_residues
MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRSVLEWIPSLGINFTVYIDGLG
LLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFMGAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSR
YGAAKSLLITVSGGLCMLGGFILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAP
TPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKSILAFSTVSQLGMIISMLGVS
AAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFN
GFLSKEMFFTSMLRVTHFDLFNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVIL
VALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVIGTIGYLSLNKWKGIYKLFPS
KLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFAGFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISA
TVATVFARSRLTAIIALGVVGYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLG
VGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTIAALGIYSMIKTKVKEEGKSG
E
>Mature_801_residues
MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRSVLEWIPSLGINFTVYIDGLG
LLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFMGAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSR
YGAAKSLLITVSGGLCMLGGFILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAP
TPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKSILAFSTVSQLGMIISMLGVS
AAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFN
GFLSKEMFFTSMLRVTHFDLFNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVIL
VALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVIGTIGYLSLNKWKGIYKLFPS
KLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFAGFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISA
TVATVFARSRLTAIIALGVVGYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLG
VGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTIAALGIYSMIKTKVKEEGKSG
E

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family

Homologues:

Organism=Homo sapiens, GI251831117, Length=365, Percent_Identity=33.1506849315069, Blast_Score=176, Evalue=6e-44,
Organism=Escherichia coli, GI1788614, Length=449, Percent_Identity=32.293986636971, Blast_Score=214, Evalue=3e-56,
Organism=Escherichia coli, GI1788829, Length=426, Percent_Identity=31.924882629108, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=32.2314049586777, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788827, Length=423, Percent_Identity=27.8959810874704, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788613, Length=489, Percent_Identity=26.9938650306748, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI2367154, Length=423, Percent_Identity=24.822695035461, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693160, Length=352, Percent_Identity=25.5681818181818, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRPA_BACSU (Q9K2S2)

Other databases:

- EMBL:   Z93937
- EMBL:   AL009126
- PIR:   A70010
- EnsemblBacteria:   EBBACT00000000025
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31600
- NMPDR:   fig|224308.1.peg.3163
- GenoList:   BSU31600
- GeneTree:   EBGT00050000000597
- HOGENOM:   HBG672487
- PhylomeDB:   Q9K2S2
- ProtClustDB:   PRK12645
- BioCyc:   BSUB:BSU31600-MONOMER
- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393
- PRINTS:   PR01435
- TIGRFAMs:   TIGR00940

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89532; Mature: 89532

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1169d9cc)-; HASH(0x11e9277c)-; HASH(0x11b08e54)-; HASH(0x11ec2664)-; HASH(0xfadef7c)-; HASH(0x11cf0504)-; HASH(0x11162558)-; HASH(0x11824f6c)-; HASH(0x11da1f88)-; HASH(0x11bfffd4)-; HASH(0x113d67f4)-; HASH(0x11e3ad40)-; HASH(0x11dac79c)-; HASH(0x11806990)-; HASH(0x117c3660)-; HASH(0x11ac9660)-; HASH(0x11769f88)-; HASH(0xe486800)-; HASH(0x11b1090c)-; HASH(0x117660ec)-; HASH(0x11b382f8)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VLEWIPSLGINFTVYIDGLGLLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFM
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSRYGAAKSLLITVSGGLCMLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCHHHHHH
FILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAP
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKS
CCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ILAFSTVSQLGMIISMLGVSAAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFNGFLSKEMFFTSMLRVTHFDL
ECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
FNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVIL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
VALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEHHHCCHHHHHHHHHCHHHH
GTIGYLSLNKWKGIYKLFPSKLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFA
HHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISATVATVFARSRLTAIIALGVV
HHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHH
VGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
AALGIYSMIKTKVKEEGKSGE
HHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure
MQLLHLAILSPFLFAFIIPFLAKYAKRVHTGWFVLILPVLLFIYFLPMIRMTQSGETLRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VLEWIPSLGINFTVYIDGLGLLFALLITGIGSLVTLYSIFYLSKEKEQLGPFYVYLLMFM
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GAMLGVVLVDNVMVLYMFWELTSLSSFLLIGYWYKREKSRYGAAKSLLITVSGGLCMLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCHHHHHH
FILLYLITDSFSIREMVHQVQLIAGHELFIPAMILILLGAFTKSAQFPFYIWLPDAMEAP
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGIYVIARFSPIFAFSAQWFWIVSLVGLFTMVWGSFHAVKQTDLKS
CCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ILAFSTVSQLGMIISMLGVSAAALHYGHTEYYTVAAMAAIFHLINHATFKGSLFMAVGII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DHETGTRDIRKLGGLMAIMPITFTISLIGTFSMAGLPPFNGFLSKEMFFTSMLRVTHFDL
ECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
FNVQTWGVLFPLFAWIGSVFTFIYSMKLLFKTFRGNYQPEQLEKQAHEAPVGMLVPPVIL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
VALAVSLFFFPNILSYSLIEPAMNSIYPTLLDGHEKFHVHISQWHGVTTELLMTAGIVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEHHHCCHHHHHHHHHCHHHH
GTIGYLSLNKWKGIYKLFPSKLTLNRLYDKLLTMMEKGSYRVTKQYMTGFLRDYLLYIFA
HHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GFIILIGGAFAIKGGFSFKTEGMAKIGVYEIILTLVMISATVATVFARSRLTAIIALGVV
HHHHHHCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYTLALFFVIFRAPDLALTQLVIETISVALFLLCFYHLPKLRLKTKTRTFRMTNFIISLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHH
VGVIVTLLGIASSSQRTKDSIASFFVKHSHDLGGGDNVVNVILVDFRGFDTMFEITVLTI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
AALGIYSMIKTKVKEEGKSGE
HHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194