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The map label for this gene is maeN

Identifier: 16080210

GI number: 16080210

Start: 3244770

End: 3246116

Strand: Direct

Name: maeN

Synonym: BSU31580

Alternate gene names: 16080210

Gene position: 3244770-3246116 (Clockwise)

Preceding gene: 16080209

Following gene: 161511068

Centisome position: 76.97

GC content: 42.84

Gene sequence:

>1347_bases
ATGGGAGCAATTCCAAAAACGGGGACAATTTCACCTGAGCAAAAAGACTCACAGGAGAAAAACCTGTTTCAAAAAATTTG
GTCTTGGGAAATCGGTGTGATTCCTTTGCCATTATACACAGTATTAGCTGTTATTATTATTCTTGCAGCATATTACAATG
AGCTGCCGGCAAATATGCTTGGCGGGTTTGCCATCATTATGATTCTTGGTGTGTTTCTCGGAGACATAGGCCAACGCATA
CCGATATTGAAGGATATTGGCGGCCCTGCTATTTTATCTTTGTTTGTTCCTTCATTTTTAGTCTTTTACAATGTACTGAA
TTCAACATCTTTAGACGCTGTAACCAACTTGATGAAAACGTCTAACTTCCTTTATTTCTATATCGCTTGCCTTGTTGTGG
GAAGTATCCTCGGTATGAACAGAATTGTTTTAATACAGGGGTTTATCAGAATGTTCGTTCCACTGGTTGCAGGAACGATC
GCGGCCGTTGCAGCCGGCATCCTTGTCGGATTTATTTTTGGCTATAGTGCCTATGATTCCTTTTTCTTTGTTGTTGTCCC
GATTATTGCCGGGGGAATAGGTGAAGGGATTTTGCCGCTTTCAATCGCCTATTCACAAATACTGGGCTCATCCGCCGATG
TATTTGTGTCTCAGCTGGTGCCAGCCGCAATTATAGGAAACGTTTTTGCTATCATATGTGCAGCCTTAATGAAAAAGCTT
GGCGACAAGCGTCCAGATCTAAATGGGAACGGACGTCTCGTAAAATCTAAAAAAGCAAATGAGATTTTCAATCAAAAAGA
AGCCGAAGCAAAAATTGATTTTAAACTAATGGGAGCAGGAGTTCTGCTCGCGTGTACATTCTTTATTTTTGGCGGGTTAT
TGGAGAAATTCATCTTCATCCCGGGCGCGATCTTAATGATTATTTCAGCTGCGGCTGTCAAGTATGCAAATATTCTTCCG
AAAAAAATGGAAGAAGGCGCTTACCAGCTTTATAAGTTTATTTCTTCAAGCTTTACATGGCCGCTGATGGTCGGTCTCGG
CATCCTCTTTATTCCGTTGGATGACGTAGCGTCTGTGATTTCTATACCGTTTGTCATCATCTGTATTTCAGTTGTTATCG
CGATGATTGGTTCTGGATATTTCGTAGGCAAGCTGATGAACATGTATCCGGTTGAATCCGCAATCGTTACCTGCTGCCAC
AGCGGTCTCGGCGGAACGGGTGATGTTGCCATTTTATCGGCATCAGGAAGAATGGGCCTTATGCCGTTTGCCCAAATTTC
AACCCGTCTCGGCGGTGCAGGCACAGTTATTTGCGCGACAGTGCTGCTTCGTTTTTTCACTTCATAA

Upstream 100 bases:

>100_bases
CCCCTTAATTTAATTGTTTATTAGTTTTTTAACTTAAAAAAATATGAAGTGTTAACGCTTTCTTGTAGACTGTAACAAAC
AATACATGGGGGTGTATGAC

Downstream 100 bases:

>100_bases
ACAGAAAGAGTTCCGTTTTATGCGGAACTCTTTTTTTATTTATTCACAATCCGAATATACTCTCGGGGACCAAACGGCAG
ATCCTTTGATTCCGTCTCAC

Product: Na+/malate symporter

Products: NA

Alternate protein names: Sodium-dependent malate transporter

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MGAIPKTGTISPEQKDSQEKNLFQKIWSWEIGVIPLPLYTVLAVIIILAAYYNELPANMLGGFAIIMILGVFLGDIGQRI
PILKDIGGPAILSLFVPSFLVFYNVLNSTSLDAVTNLMKTSNFLYFYIACLVVGSILGMNRIVLIQGFIRMFVPLVAGTI
AAVAAGILVGFIFGYSAYDSFFFVVVPIIAGGIGEGILPLSIAYSQILGSSADVFVSQLVPAAIIGNVFAIICAALMKKL
GDKRPDLNGNGRLVKSKKANEIFNQKEAEAKIDFKLMGAGVLLACTFFIFGGLLEKFIFIPGAILMIISAAAVKYANILP
KKMEEGAYQLYKFISSSFTWPLMVGLGILFIPLDDVASVISIPFVIICISVVIAMIGSGYFVGKLMNMYPVESAIVTCCH
SGLGGTGDVAILSASGRMGLMPFAQISTRLGGAGTVICATVLLRFFTS

Sequences:

>Translated_448_residues
MGAIPKTGTISPEQKDSQEKNLFQKIWSWEIGVIPLPLYTVLAVIIILAAYYNELPANMLGGFAIIMILGVFLGDIGQRI
PILKDIGGPAILSLFVPSFLVFYNVLNSTSLDAVTNLMKTSNFLYFYIACLVVGSILGMNRIVLIQGFIRMFVPLVAGTI
AAVAAGILVGFIFGYSAYDSFFFVVVPIIAGGIGEGILPLSIAYSQILGSSADVFVSQLVPAAIIGNVFAIICAALMKKL
GDKRPDLNGNGRLVKSKKANEIFNQKEAEAKIDFKLMGAGVLLACTFFIFGGLLEKFIFIPGAILMIISAAAVKYANILP
KKMEEGAYQLYKFISSSFTWPLMVGLGILFIPLDDVASVISIPFVIICISVVIAMIGSGYFVGKLMNMYPVESAIVTCCH
SGLGGTGDVAILSASGRMGLMPFAQISTRLGGAGTVICATVLLRFFTS
>Mature_447_residues
GAIPKTGTISPEQKDSQEKNLFQKIWSWEIGVIPLPLYTVLAVIIILAAYYNELPANMLGGFAIIMILGVFLGDIGQRIP
ILKDIGGPAILSLFVPSFLVFYNVLNSTSLDAVTNLMKTSNFLYFYIACLVVGSILGMNRIVLIQGFIRMFVPLVAGTIA
AVAAGILVGFIFGYSAYDSFFFVVVPIIAGGIGEGILPLSIAYSQILGSSADVFVSQLVPAAIIGNVFAIICAALMKKLG
DKRPDLNGNGRLVKSKKANEIFNQKEAEAKIDFKLMGAGVLLACTFFIFGGLLEKFIFIPGAILMIISAAAVKYANILPK
KMEEGAYQLYKFISSSFTWPLMVGLGILFIPLDDVASVISIPFVIICISVVIAMIGSGYFVGKLMNMYPVESAIVTCCHS
GLGGTGDVAILSASGRMGLMPFAQISTRLGGAGTVICATVLLRFFTS

Specific function: Acts as a Na(+)-malate symporter, as it catalyzes malate-dependent uptake of Na(+) and Na(+)-dependent uptake of malate

COG id: COG3493

COG function: function code C; Na+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:citrate (SCF) symporter family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAEN_BACSU (O05256)

Other databases:

- EMBL:   Z93937
- EMBL:   AL009126
- PIR:   G70009
- RefSeq:   NP_391036.1
- ProteinModelPortal:   O05256
- EnsemblBacteria:   EBBACT00000001424
- GeneID:   938855
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31580
- NMPDR:   fig|224308.1.peg.3161
- GenoList:   BSU31580
- GeneTree:   EBGT00050000001855
- HOGENOM:   HBG304840
- OMA:   MGLMPFA
- ProtClustDB:   CLSK877154
- BioCyc:   BSUB:BSU31580-MONOMER
- InterPro:   IPR004679
- InterPro:   IPR018025
- TIGRFAMs:   TIGR00783

Pfam domain/function: PF03390 2HCT

EC number: NA

Molecular weight: Translated: 47825; Mature: 47694

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10609b34)-; HASH(0x11d2584c)-; HASH(0x121619fc)-; HASH(0x11cb14b8)-; HASH(0x11c52f20)-; HASH(0x12081884)-; HASH(0x11ed5a88)-; HASH(0x121436ac)-; HASH(0xf56b6c4)-; HASH(0x11c64ea4)-; HASH(0x105262c0)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHCCCCHHHHHHHHHHHHHCH
>Mature Secondary Structure 
GAIPKTGTISPEQKDSQEKNLFQKIWSWEIGVIPLPLYTVLAVIIILAAYYNELPANML
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
GGFAIIMILGVFLGDIGQRIPILKDIGGPAILSLFVPSFLVFYNVLNSTSLDAVTNLMKT
HHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SNFLYFYIACLVVGSILGMNRIVLIQGFIRMFVPLVAGTIAAVAAGILVGFIFGYSAYDS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFFVVVPIIAGGIGEGILPLSIAYSQILGSSADVFVSQLVPAAIIGNVFAIICAALMKKL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDKRPDLNGNGRLVKSKKANEIFNQKEAEAKIDFKLMGAGVLLACTFFIFGGLLEKFIFI
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHH
PGAILMIISAAAVKYANILPKKMEEGAYQLYKFISSSFTWPLMVGLGILFIPLDDVASVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECHHHHHHHH
SIPFVIICISVVIAMIGSGYFVGKLMNMYPVESAIVTCCHSGLGGTGDVAILSASGRMGL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
MPFAQISTRLGGAGTVICATVLLRFFTS
CHHHHHHHHCCCCHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10903309; 12949159