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The map label for this gene is mrpB

Identifier: 16080213

GI number: 16080213

Start: 3248996

End: 3249427

Strand: Direct

Name: mrpB

Synonym: BSU31610

Alternate gene names: 16080213

Gene position: 3248996-3249427 (Clockwise)

Preceding gene: 161511068

Following gene: 255767719

Centisome position: 77.07

GC content: 44.68

Gene sequence:

>432_bases
GTGAATGAACAAAAAACAAATGACTTGATTCTTCAAACCGCGACAAAGCTTGTATCCTTTATCATTTTGCTTTTCTCTTT
CTATTTATTTTTATCAGGGCATAACGCGCCCGGAGGAGGCTTTGTCGGCGGACTGATCACGTCCTCTTCCATCGTTCTCT
TGCTCTTGGCATATGATTTAAAAACCGTGCGTTCCCTTTTGCCTGTCAATTTCATTTATGTCGCGGGAGCCGGCCTTCTG
CTCGCTGTATTAACAGGCGTTGGCTCCTTTGTCTTCGGGGCTCCTTTTTTAACCCACACATTCGGATACTTTCAGCTGCC
GATCCTTGGGAAAACGGAGCTCGCCACAGCGACGATATTTGATTTAGGCGTTTATCTTGTTGTCGTAGGCATAACGATGA
CCATTATTCAAACGATTGGAGAGGAAGAATAA

Upstream 100 bases:

>100_bases
AGGGGATTTGATACAATGTTTGAAATTACGGTATTAACGATAGCCGCTCTCGGCATTTACAGCATGATTAAAACAAAAGT
AAAAGAGGAGGGGAAAAGCG

Downstream 100 bases:

>100_bases
TGGAAATCTTAATGGCTGTATTAGCCGGTATTATTTTTATGGCTGCTACGTATTTGCTGCTGTCTAAAAGCCTGCTTCGC
GTCATTATCGGAACAGCACT

Product: monovalent cation/H+ antiporter subunit B

Products: NA

Alternate protein names: Mrp complex subunit B; Multiple resistance and pH homeostasis protein B

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDLKTVRSLLPVNFIYVAGAGLL
LAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIFDLGVYLVVVGITMTIIQTIGEEE

Sequences:

>Translated_143_residues
MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDLKTVRSLLPVNFIYVAGAGLL
LAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIFDLGVYLVVVGITMTIIQTIGEEE
>Mature_143_residues
MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDLKTVRSLLPVNFIYVAGAGLL
LAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIFDLGVYLVVVGITMTIIQTIGEEE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG2111

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhB subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRPB_BACSU (O05259)

Other databases:

- EMBL:   Z93937
- EMBL:   AL009126
- PIR:   B70010
- RefSeq:   NP_391039.1
- ProteinModelPortal:   O05259
- EnsemblBacteria:   EBBACT00000002425
- GeneID:   937177
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31610
- NMPDR:   fig|224308.1.peg.3164
- GenoList:   BSU31610
- GeneTree:   EBGT00050000001379
- HOGENOM:   HBG313662
- OMA:   VLLRGHN
- ProtClustDB:   PRK12573
- BioCyc:   BSUB:BSU31610-MONOMER
- InterPro:   IPR005281
- InterPro:   IPR007182
- TIGRFAMs:   TIGR00943

Pfam domain/function: PF04039 MnhB

EC number: NA

Molecular weight: Translated: 15294; Mature: 15294

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x108a801c)-; HASH(0x11effc68)-; HASH(0x12143d94)-; HASH(0x1213db68)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KTVRSLLPVNFIYVAGAGLLLAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHCCCCCCCHHHHHHHHH
DLGVYLVVVGITMTIIQTIGEEE
HHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MNEQKTNDLILQTATKLVSFIILLFSFYLFLSGHNAPGGGFVGGLITSSSIVLLLLAYDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KTVRSLLPVNFIYVAGAGLLLAVLTGVGSFVFGAPFLTHTFGYFQLPILGKTELATATIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHCCCCCCCHHHHHHHHH
DLGVYLVVVGITMTIIQTIGEEE
HHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194