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The map label for this gene is yugF

Identifier: 16080194

GI number: 16080194

Start: 3227581

End: 3228402

Strand: Direct

Name: yugF

Synonym: BSU31420

Alternate gene names: 16080194

Gene position: 3227581-3228402 (Clockwise)

Preceding gene: 16080190

Following gene: 16080196

Centisome position: 76.56

GC content: 45.01

Gene sequence:

>822_bases
ATGGAGGCGGTTTCACCAATAAGGCGGTTTACGGTCGATGGAGTCAATGTATACTACGAACATTATCAAAATCCCGGCAG
GCAAACGCTGGTCTGCGTACACGGTTTTTTATCATCTGCATTCAGCTTCAGAAAAGTAATTCCTCTCCTTCGGGACAAGT
ACGACATCATCGCGCTTGATTTGCCTCCTTTCGGCCAATCTGAAAAATCAAGAACGTTTATCTATACCTATCAAAACCTT
GCTAAGCTTGTCATTGGGATTTTGGAGCACTTGCAAGTGAAACAGGCTGTGCTTGTCGGGCATTCTATGGGCGGGCAGAT
ATCGCTGTCTGCTGCTCTCCAAAAACCTGAGCTTTTTTCAAAGGTTGTGCTGCTTTGCAGTTCAGGGTATTTAAAACGTT
CACACCCGACGATTATTTTCGGGACCCATATCCCGTATTTTCATCTTTACATCAAACGCTGGCTCTCGAAAGAAGGCGTG
ATGAAAAATTTATTAAATGTGGTGCATGACAAATCGCTGATTGATGAGGAGATGATTGACGGCTATGGCAGACCGTTTCA
GGACGAGCAGATTTTCAAAGCCATGACAAGGTTTATCCGCCACAGAGAAGGAGATTTAGAACCCGAGCAATTGAAGAAAA
TGAACAAGCCTGCTCTATTGATTTGGGGCGAGGAGGATCGAATTGTCCCAATGGAGATCGGTAAACGGCTGCACGCAGAT
TTGCCTAATTCCGTGCTGTACTCACTTGGCCAGACCGGACATCTGGTGCCTGAAGAACGGCCTGAACTTATTTCTGAACA
CATTGCTGATTTTATCAAATAA

Upstream 100 bases:

>100_bases
CGTTTTTTGACGAATGCTAAGAAAAAACGAGGAGAAACTTCCGCTAGAATTGACAATTTCAGGGCATAATACAACCATCA
AAAAAAGGGAGTGACACAGA

Downstream 100 bases:

>100_bases
CAAAAAAACCGGTGCTGAACCGGTTTTTTTAAGGCGTGCAGGAGCTGCTGTTTTTAATGAATAAAAGCTGGCCAGCCACT
TCCCGGCAGCTGGCGATCGG

Product: hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEAVSPIRRFTVDGVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNL
AKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGV
MKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHAD
LPNSVLYSLGQTGHLVPEERPELISEHIADFIK

Sequences:

>Translated_273_residues
MEAVSPIRRFTVDGVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNL
AKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGV
MKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHAD
LPNSVLYSLGQTGHLVPEERPELISEHIADFIK
>Mature_273_residues
MEAVSPIRRFTVDGVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNL
AKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGV
MKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHAD
LPNSVLYSLGQTGHLVPEERPELISEHIADFIK

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family

Homologues:

Organism=Caenorhabditis elegans, GI17554012, Length=284, Percent_Identity=21.830985915493, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324392, Length=284, Percent_Identity=24.6478873239437, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUGF_BACSU (O05235)

Other databases:

- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   E70010
- RefSeq:   NP_391020.1
- ProteinModelPortal:   O05235
- SMR:   O05235
- EnsemblBacteria:   EBBACT00000002105
- GeneID:   937169
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31420
- NMPDR:   fig|224308.1.peg.3145
- GenoList:   BSU31420
- GeneTree:   EBGT00070000031954
- HOGENOM:   HBG338260
- OMA:   FIRHREG
- ProtClustDB:   CLSK873275
- BioCyc:   BSUB:BSU31420-MONOMER
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31326; Mature: 31326

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: ACT_SITE 102-102 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVSPIRRFTVDGVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALD
CCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LPPFGQSEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFS
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHH
KVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDEEMID
HHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHAD
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCC
LPNSVLYSLGQTGHLVPEERPELISEHIADFIK
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MEAVSPIRRFTVDGVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALD
CCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LPPFGQSEKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFS
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHH
KVVLLCSSGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDEEMID
HHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHAD
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCC
LPNSVLYSLGQTGHLVPEERPELISEHIADFIK
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377