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The map label for this gene is patB

Identifier: 16080196

GI number: 16080196

Start: 3228778

End: 3229941

Strand: Direct

Name: patB

Synonym: BSU31440

Alternate gene names: 16080196

Gene position: 3228778-3229941 (Clockwise)

Preceding gene: 16080194

Following gene: 255767715

Centisome position: 76.59

GC content: 48.45

Gene sequence:

>1164_bases
ATGAACTTTGATAAACGAGAAGAACGCCTTGGCACCCAATCGGTCAAATGGGATAAAACAGGCGAATTATTTGGCGTGAC
AGACGCCCTCCCGATGTGGGTGGCAGATATGGATTTTCGCGCCCCGGAAGCGATAACTGAAGCGTTAAAAGAGCGTCTTG
ACCACGGTATTTTTGGTTATACAACTCCAGATCAAAAAACGAAGGATGCTGTGTGCGGATGGATGCAAAACAGGCACGGC
TGGAAGGTAAATCCGGAAAGCATCACATTCAGCCCCGGCGTTGTAACAGCTTTGAGCATGGCGGTACAAGCTTTTACGGA
GCCGGGCGATCAAGTAGTTGTTCAGCCTCCAGTTTATACGCCTTTTTACCATATGGTGGAGAAAAACGGCCGGCACATTT
TACATAATCCGCTGCTAGAAAAAGACGGCGCATATGCAATCGATTTTGAAGATTTGGAGACAAAGCTTAGTGACCCAAGC
GTTACATTGTTTATTTTATGCAATCCTCATAACCCTTCTGGGCGTTCATGGAGCCGGGAAGATTTACTGAAGCTAGGCGA
ATTGTGCCTTGAGCATGGCGTCACAGTTGTATCGGATGAAATCCATTCCGATTTAATGCTGTACGGACATAAACACACGC
CGTTCGCTTCGCTCTCTGACGATTTCGCTGATATTTCCGTGACATGTGCTGCTCCAAGCAAAACATTTAATATCGCCGGA
TTGCAGGCTTCAGCGATCATTATTCCGGATCGCCTGAAGCGCGCCAAGTTTTCCGCAAGCCTTCAGCGAAATGGTTTAGG
CGGACTGAACGCGTTTGCCGTCACTGCAATCGAAGCCGCGTATTCAAAAGGCGGACCGTGGCTTGATGAATTGATCACTT
ACATTGAGAAAAACATGAACGAAGCGGAAGCCTTTTTGAGCACCGAGCTGCCAAAGGTCAAAATGATGAAGCCGGATGCA
TCATACTTGATTTGGCTCGACTTCAGCGCTTACGGCTTGTCCGATGCAGAGCTTCAGCAAAGAATGCTAAAAAAAGGAAA
AGTTATTTTAGAGCCCGGGACGAAATACGGGCCTGGCGGAGAAGGATTTATGCGCCTGAACGCCGGATGCTCTCTTGCAA
CCCTGCAGGACGGCCTGCGCCGCATCAAAGCCGCATTATCGTAA

Upstream 100 bases:

>100_bases
TGACTTTCTTCCATATTCATCACCGCTTATAATAAAATTTGCAGTTCTATCTTATCATAGTGAAGCTTAGAAGAATAAAC
ATACATGAGGGGGTAACAGA

Downstream 100 bases:

>100_bases
ATATAGCTGTAAACGCCTTTACGTCTTCATTTGTAAAGGCGTTCTTAATAAAGGAATTTTATATTTTACTTCTAATATTT
AGTGTTATAATAGATTCATA

Product: C-S lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGYTTPDQKTKDAVCGWMQNRHG
WKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYTPFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPS
VTLFILCNPHNPSGRSWSREDLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAG
LQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMNEAEAFLSTELPKVKMMKPDA
SYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGGEGFMRLNAGCSLATLQDGLRRIKAALS

Sequences:

>Translated_387_residues
MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGYTTPDQKTKDAVCGWMQNRHG
WKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYTPFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPS
VTLFILCNPHNPSGRSWSREDLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAG
LQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMNEAEAFLSTELPKVKMMKPDA
SYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGGEGFMRLNAGCSLATLQDGLRRIKAALS
>Mature_387_residues
MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGYTTPDQKTKDAVCGWMQNRHG
WKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYTPFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPS
VTLFILCNPHNPSGRSWSREDLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAG
LQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMNEAEAFLSTELPKVKMMKPDA
SYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGGEGFMRLNAGCSLATLQDGLRRIKAALS

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily

Homologues:

Organism=Homo sapiens, GI56713254, Length=269, Percent_Identity=25.2788104089219, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI56713256, Length=269, Percent_Identity=25.2788104089219, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI4507369, Length=334, Percent_Identity=24.251497005988, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI95147551, Length=392, Percent_Identity=23.2142857142857, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI169881279, Length=392, Percent_Identity=23.2142857142857, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787909, Length=382, Percent_Identity=33.5078534031414, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1788627, Length=299, Percent_Identity=24.7491638795987, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787710, Length=301, Percent_Identity=23.9202657807309, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786816, Length=219, Percent_Identity=24.2009132420091, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788722, Length=338, Percent_Identity=23.6686390532544, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567663, Length=316, Percent_Identity=24.3670886075949, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17567369, Length=334, Percent_Identity=24.5508982035928, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994476, Length=263, Percent_Identity=24.3346007604563, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994472, Length=263, Percent_Identity=24.3346007604563, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322401, Length=337, Percent_Identity=22.2551928783383, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI18859735, Length=369, Percent_Identity=21.680216802168, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573069, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646114, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573067, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573065, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBL_BACSU (Q08432)

Other databases:

- EMBL:   U63302
- EMBL:   Z93933
- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   S32934
- RefSeq:   NP_391022.1
- ProteinModelPortal:   Q08432
- SMR:   Q08432
- EnsemblBacteria:   EBBACT00000002936
- GeneID:   937170
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31440
- NMPDR:   fig|224308.1.peg.3147
- GenoList:   BSU31440
- GeneTree:   EBGT00070000031804
- HOGENOM:   HBG681988
- OMA:   NEVCDVN
- PhylomeDB:   Q08432
- ProtClustDB:   CLSK887791
- BioCyc:   BSUB:BSU31440-MONOMER
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.4.1.8

Molecular weight: Translated: 42789; Mature: 42789

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGY
CCCCHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
TTPDQKTKDAVCGWMQNRHGWKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYT
CCCCCCHHHHHHHHHHCCCCCEECCCCEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCC
PFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPSVTLFILCNPHNPSGRSWSRE
HHHHHHHCCCCEEECCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHH
DLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAG
HHHHHHHHHHHCCCEEEEHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEC
LQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMN
CEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
EAEAFLSTELPKVKMMKPDASYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGG
HHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCC
EGFMRLNAGCSLATLQDGLRRIKAALS
CCEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGY
CCCCHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
TTPDQKTKDAVCGWMQNRHGWKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYT
CCCCCCHHHHHHHHHHCCCCCEECCCCEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCC
PFYHMVEKNGRHILHNPLLEKDGAYAIDFEDLETKLSDPSVTLFILCNPHNPSGRSWSRE
HHHHHHHCCCCEEECCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHH
DLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAG
HHHHHHHHHHHCCCEEEEHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEC
LQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMN
CEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
EAEAFLSTELPKVKMMKPDASYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGG
HHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCC
EGFMRLNAGCSLATLQDGLRRIKAALS
CCEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377