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The map label for this gene is opuD

Identifier: 16080059

GI number: 16080059

Start: 3076818

End: 3078356

Strand: Direct

Name: opuD

Synonym: BSU30070

Alternate gene names: 16080059

Gene position: 3076818-3078356 (Clockwise)

Preceding gene: 255767688

Following gene: 16080067

Centisome position: 72.99

GC content: 45.16

Gene sequence:

>1539_bases
ATGTTGAAACATATATCTAGTGTATTTTGGATTGTGATTGCAATCACAGCTGCAGCTGTATTATGGGGTGTTATTTCTCC
TGACAGCCTGCAAAATGTGTCTCAATCGGCACAAGCATTTATTACCGATTCATTTGGTTGGTATTATCTTTTAGTTGTCT
CTTTGTTTGTCGGGTTTTGTCTCTTTTTGATTTTCAGCCCGATCGGAAAAATCAAACTCGGAAAGCCTGATGAAAAACCG
GAATTCGGGTTATTATCATGGTTTGCCATGCTGTTCAGCGCAGGCATGGGAATCGGCTTAGTATTCTACGGTGCGGCTGA
ACCGATCAGCCATTACGCAATTAGCTCTCCATCAGGTGAAACAGAAACCCCCCAAGCCTTCAGAGATGCACTCCGCTATA
CGTTTTTCCACTGGGGTTTGCACGCTTGGGCCATTTATGCCATTGTGGCATTATGCATTGCCTATTTCCAATTCCGCAAA
GGCGCACCCGGGTTAATCAGCAGCACACTGTCACCGATTCTCGGGGATAAAGTAAACGGACCGATCGGAAAGGCCATTGA
CTGTATTGCAGTTTTCGCCACTGTTGTCGGGGTGTCAACAAGTCTTGGTCTTGGCGCAACCCAAATTAACGGCGGTTTGA
ATTACCTGTTCGGCATTCCGAATGCGTTTATCGTCCAATTGGTTTTAATCATTATTGTGACAGTGCTCTTTTTACTTTCT
GCGTGGAGCGGACTCGGAAAAGGAATTAAGTATTTAAGCAACACAAACATGGTATTGGCCGGATTGTTAATGTTGTTTAT
GCTCGTTGTCGGGCCTACAGTGCTGATAATGAACTCATTTACAGATTCTATCGGACAATACATTCAAAATATCGTTCAAA
TGAGCTTCAGACTGACACCGAACGATCCTGAAAAACGCGAATGGATTAACAGCTGGACGATTTTCTATTGGGCATGGTGG
ATTTCCTGGTCTCCATTTGTCGGCATTTTCATCGCCCGTGTATCCAGAGGCCGGACGATCCGCGAATTTTTAATCGGCGT
CTTGGTGACACCTTGTATTTTGACATTTCTATGGTTCTCCATTTTCGGCGTATCAGCCATGGATCTCCAGCAAAAAGGCG
CGTTCAATGTAGCAAAGCTGTCCACTGAAACGATGCTGTTCGGAACACTTGATCACTATCCGCTGACAATGGTGACATCG
ATCCTTGCATTGATTCTGATCGCAGTATTCTTTATCACGTCAGCTGATTCAGCTACATTCGTGCTGGGCATGCAGACGTC
GTACGGTTCTTTAAATCCGGCGAATTCTGTGAAACTCAGCTGGGGTATCATTCAGTCCGCCATGGCGGCTGTGCTCCTAT
ATTCCGGAGGGCTTGCCGCTTTGCAGAACACAGCGATTCTGGCAGCGCTGCCGTTTTCTATCGTTATCCTGCTGATGATT
GCCTCTCTCTATCAATCGCTCTCAAAAGAAAGAAGAGAGATCAAAAAAGCGGAGAAACTCGATAAACCAAGAAGTCCAAG
AGTGAAAAAAGCATATTAA

Upstream 100 bases:

>100_bases
ATTGCGGGGTAATTTTAAGAGAGGAATGAGCTGCCATCGTACGACCATACTAAGCCTGCGAGCAAGATATAGGCGTACAC
AAAAAATTAGAGGGTGAGAA

Downstream 100 bases:

>100_bases
CAAAAAAGATCTTTCCGCGCCTGCGGAAAGATCTTTTTATTTGCGATATAAAATAAGGGCGGAAAAACAGTAAAGCTGCT
CGCCGTCTGGGTCGCACGCC

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLKHISSVFWIVIAITAAAVLWGVISPDSLQNVSQSAQAFITDSFGWYYLLVVSLFVGFCLFLIFSPIGKIKLGKPDEKP
EFGLLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETPQAFRDALRYTFFHWGLHAWAIYAIVALCIAYFQFRK
GAPGLISSTLSPILGDKVNGPIGKAIDCIAVFATVVGVSTSLGLGATQINGGLNYLFGIPNAFIVQLVLIIIVTVLFLLS
AWSGLGKGIKYLSNTNMVLAGLLMLFMLVVGPTVLIMNSFTDSIGQYIQNIVQMSFRLTPNDPEKREWINSWTIFYWAWW
ISWSPFVGIFIARVSRGRTIREFLIGVLVTPCILTFLWFSIFGVSAMDLQQKGAFNVAKLSTETMLFGTLDHYPLTMVTS
ILALILIAVFFITSADSATFVLGMQTSYGSLNPANSVKLSWGIIQSAMAAVLLYSGGLAALQNTAILAALPFSIVILLMI
ASLYQSLSKERREIKKAEKLDKPRSPRVKKAY

Sequences:

>Translated_512_residues
MLKHISSVFWIVIAITAAAVLWGVISPDSLQNVSQSAQAFITDSFGWYYLLVVSLFVGFCLFLIFSPIGKIKLGKPDEKP
EFGLLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETPQAFRDALRYTFFHWGLHAWAIYAIVALCIAYFQFRK
GAPGLISSTLSPILGDKVNGPIGKAIDCIAVFATVVGVSTSLGLGATQINGGLNYLFGIPNAFIVQLVLIIIVTVLFLLS
AWSGLGKGIKYLSNTNMVLAGLLMLFMLVVGPTVLIMNSFTDSIGQYIQNIVQMSFRLTPNDPEKREWINSWTIFYWAWW
ISWSPFVGIFIARVSRGRTIREFLIGVLVTPCILTFLWFSIFGVSAMDLQQKGAFNVAKLSTETMLFGTLDHYPLTMVTS
ILALILIAVFFITSADSATFVLGMQTSYGSLNPANSVKLSWGIIQSAMAAVLLYSGGLAALQNTAILAALPFSIVILLMI
ASLYQSLSKERREIKKAEKLDKPRSPRVKKAY
>Mature_512_residues
MLKHISSVFWIVIAITAAAVLWGVISPDSLQNVSQSAQAFITDSFGWYYLLVVSLFVGFCLFLIFSPIGKIKLGKPDEKP
EFGLLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETPQAFRDALRYTFFHWGLHAWAIYAIVALCIAYFQFRK
GAPGLISSTLSPILGDKVNGPIGKAIDCIAVFATVVGVSTSLGLGATQINGGLNYLFGIPNAFIVQLVLIIIVTVLFLLS
AWSGLGKGIKYLSNTNMVLAGLLMLFMLVVGPTVLIMNSFTDSIGQYIQNIVQMSFRLTPNDPEKREWINSWTIFYWAWW
ISWSPFVGIFIARVSRGRTIREFLIGVLVTPCILTFLWFSIFGVSAMDLQQKGAFNVAKLSTETMLFGTLDHYPLTMVTS
ILALILIAVFFITSADSATFVLGMQTSYGSLNPANSVKLSWGIIQSAMAAVLLYSGGLAALQNTAILAALPFSIVILLMI
ASLYQSLSKERREIKKAEKLDKPRSPRVKKAY

Specific function: High-affinity uptake of glycine betaine

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family

Homologues:

Organism=Escherichia coli, GI1786506, Length=472, Percent_Identity=40.2542372881356, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788102, Length=466, Percent_Identity=30.9012875536481, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786224, Length=479, Percent_Identity=29.8538622129436, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OPUD_BACSU (P54417)

Other databases:

- EMBL:   U50082
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69670
- RefSeq:   NP_390885.1
- ProteinModelPortal:   P54417
- SMR:   P54417
- EnsemblBacteria:   EBBACT00000002676
- GeneID:   937986
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30070
- NMPDR:   fig|224308.1.peg.3010
- GenoList:   BSU30070
- GeneTree:   EBGT00050000001154
- HOGENOM:   HBG682943
- OMA:   TIGSYIQ
- PhylomeDB:   P54417
- ProtClustDB:   CLSK887742
- BioCyc:   BSUB:BSU30070-MONOMER
- InterPro:   IPR000060
- InterPro:   IPR018093
- TIGRFAMs:   TIGR00842

Pfam domain/function: PF02028 BCCT

EC number: NA

Molecular weight: Translated: 56123; Mature: 56123

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11803b24)-; HASH(0x11b7a598)-; HASH(0x1140b538)-; HASH(0x11afeb60)-; HASH(0x11a96e28)-; HASH(0xf9246c8)-; HASH(0xfd9acb8)-; HASH(0x11cb7378)-; HASH(0x11c9122c)-; HASH(0x11b27ce8)-; HASH(0x11bdcf3c)-; HASH(0x11a54078)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377