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The map label for this gene is ypdQ

Identifier: 16079258

GI number: 16079258

Start: 2310419

End: 2310817

Strand: Direct

Name: ypdQ

Synonym: BSU21990

Alternate gene names: 16079258

Gene position: 2310419-2310817 (Clockwise)

Preceding gene: 16079257

Following gene: 50812255

Centisome position: 54.81

GC content: 42.61

Gene sequence:

>399_bases
ATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCAGGGCCTTCCGGTATCGGCATTTTTATCAAACATGA
GGGAAAAGCAGAATCTTTCTCTATTCCAATCGGAGTGCATACAAACCAGGAAGCGGAATTTCTTGCATTAATTGAAGGGA
TGAAGCTATGTGCAACGCGCGGGTATCAATCAGTTTCCTTTCGAACCGATTCAGATATTGTGGAACGCGCCACTGAGCTT
GAAATGGTTAAAAATATAACGTTTCAACCGTTTGTAGAAGAGATCATCCGATTAAAAGCGGCATTCCCTCTTTTTTTCAT
CAAATGGATACCGGGCAAGCAAAACCAAAAAGCCGATCTTCTGGCAAAAGAAGCGATTCGGCTGAATGAAAAGAATTAA

Upstream 100 bases:

>100_bases
TTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTAATTTCATTGCCTTATGCCTATGCAGCCAAAAAAATGATACCT
AATGATGAAAGGAGTTCATA

Downstream 100 bases:

>100_bases
ATCTTGATCATTGGTTCATCCTAATGGCGAGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTT
ACTCCGCAAGGCGACCTGGA

Product: hypothetical protein

Products: 5'-phosphomonoester [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MPTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATEL
EMVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADLLAKEAIRLNEKN

Sequences:

>Translated_132_residues
MPTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATEL
EMVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADLLAKEAIRLNEKN
>Mature_131_residues
PTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATELE
MVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADLLAKEAIRLNEKN

Specific function: Not known; does not have RNase H activity

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNHL_BACSU (P54162)

Other databases:

- EMBL:   L77246
- EMBL:   AL009126
- PIR:   D69934
- RefSeq:   NP_390082.1
- ProteinModelPortal:   P54162
- SMR:   P54162
- EnsemblBacteria:   EBBACT00000001649
- GeneID:   939074
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU21990
- NMPDR:   fig|224308.1.peg.2205
- GenoList:   BSU21990
- GeneTree:   EBGT00050000002754
- HOGENOM:   HBG742437
- OMA:   TNHEAEF
- ProtClustDB:   CLSK887476
- BioCyc:   BSUB:BSU21990-MONOMER
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: 3.1.26.4 [C]

Molecular weight: Translated: 14670; Mature: 14539

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATR
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
GYQSVSFRTDSDIVERATELEMVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADL
CCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHH
LAKEAIRLNEKN
HHHHHHHCCCCC
>Mature Secondary Structure 
PTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATR
CCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
GYQSVSFRTDSDIVERATELEMVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADL
CCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHH
LAKEAIRLNEKN
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Sulfhydryl reagents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RNA-DNA hybrid; H2O [C]

Specific reaction: RNA-DNA hybrid + H2O = 5'-phosphomonoester [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: (NH4)2SO4; Dextran [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9888800