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The map label for this gene is rhcU

Identifier: 16520047

GI number: 16520047

Start: 75818

End: 76855

Strand: Reverse

Name: rhcU

Synonym: NGR_a00580

Alternate gene names: 16520047

Gene position: 76855-75818 (Counterclockwise)

Preceding gene: 16520046

Following gene: 16520048

Centisome position: 14.33

GC content: 55.49

Gene sequence:

>1038_bases
ATGAGCGATACGAGCGAAGAGAAGTCACACGGCGCTACCCCTAAGAAGCTGAGCGACGCGCGCAAAAGAGGTCAAATACC
GCGTAGCTCGGATTTCGTCCGCGCGGCCGCCACTTGCGCTGGGCTTGGCTACTTATGGCTAAGAGGTAGCGTTATCGAAG
ATAAATGCAGGGAAGCGCTACTATTAACCGACAAGCTGCAAAACCTGCCTTTCAATCTCGCGGTCCGGCAGGCGCTGGTT
CTGCTAGTCGAACTCACCCTGGCAACCGTTGGCCCGCTGCTCTCAGCTCTTTTTGGCGCGGTAATCTTGGCTGCATTACT
GGCCAATAGAGGATTTGTATTCTCTCTCGAGCCAATGAAGCCCAACTTCGATAAAATCAATCCTTTTCAATGGCTGAAGC
GCTTGGGGTCGGCGCGTTCGGCAGTCGAGGTTGGCAAAACGCTGTTCAAGGTGTTGGTTCTCGGCGGGACCTTTTCCCTC
TTCTTTCTCGGTTTATGGAAGACGATGGTCTATCTGCCGGTCTGCGGAATGGGGTGTTTCGGCGTCGTCTTTACCGGAGC
GAAACAGCTGATCGGAATTGGCGCCGGCGCACTTCTGATCGGCGGACTGATCGATCTGCTGTTGCAGCGTGCGCTATTTC
TGCGCGAAATGCGCATGACGAAGACGGAAATAAAGCGCGAGTTGAAGGAACAGCAAGGAACGCCAGAATTGAAGGGTGAA
CGGCGTCGCATTCGCAACGAGATGGCTAGCGAGCCTCCGCTTGGTGTGCATCGCGCCACGTTAGTCTACAGAGGAACTGC
GGTGCTGATCGGTCTGCGTTACGTTCGTGGTGAGACCGGAGTTCCGATCTTAGTTTGCCGCGCCGAGGGCGAGGCCGCTT
CAGATATGTTTCGCGAGGCGCAGAATCTGCGTCTCAAGATTGTCGATGATCATGTCCTAGCGCACCAACTCATGAGCACA
ACGAAACTGGGCACCGCCATCCCCATGCAATATTTCGAGCCCATCGCGAGAGCACTCTTGGCCGCAGGTTTGGCCTGA

Upstream 100 bases:

>100_bases
CGATCATCATGGTTACCTACGCTACCTATTTGCTCGAGGGCATTAAACTTGAGATCACGCAAGCGGACGGCACGCTTGGG
TGGCTCGACAAGTTGCTGAA

Downstream 100 bases:

>100_bases
GCATCCCTGAATTCGGAAAAGAACTTTGAAGACATTGACTTCCGGGCGTGATTCCTTTGGCTTTAAGTCGGGACGCAATG
GATGGCCCAGGAAGAAACAG

Product: inner membrane protein RhcU; component of type III secretion apparatus

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSDTSEEKSHGATPKKLSDARKRGQIPRSSDFVRAAATCAGLGYLWLRGSVIEDKCREALLLTDKLQNLPFNLAVRQALV
LLVELTLATVGPLLSALFGAVILAALLANRGFVFSLEPMKPNFDKINPFQWLKRLGSARSAVEVGKTLFKVLVLGGTFSL
FFLGLWKTMVYLPVCGMGCFGVVFTGAKQLIGIGAGALLIGGLIDLLLQRALFLREMRMTKTEIKRELKEQQGTPELKGE
RRRIRNEMASEPPLGVHRATLVYRGTAVLIGLRYVRGETGVPILVCRAEGEAASDMFREAQNLRLKIVDDHVLAHQLMST
TKLGTAIPMQYFEPIARALLAAGLA

Sequences:

>Translated_345_residues
MSDTSEEKSHGATPKKLSDARKRGQIPRSSDFVRAAATCAGLGYLWLRGSVIEDKCREALLLTDKLQNLPFNLAVRQALV
LLVELTLATVGPLLSALFGAVILAALLANRGFVFSLEPMKPNFDKINPFQWLKRLGSARSAVEVGKTLFKVLVLGGTFSL
FFLGLWKTMVYLPVCGMGCFGVVFTGAKQLIGIGAGALLIGGLIDLLLQRALFLREMRMTKTEIKRELKEQQGTPELKGE
RRRIRNEMASEPPLGVHRATLVYRGTAVLIGLRYVRGETGVPILVCRAEGEAASDMFREAQNLRLKIVDDHVLAHQLMST
TKLGTAIPMQYFEPIARALLAAGLA
>Mature_344_residues
SDTSEEKSHGATPKKLSDARKRGQIPRSSDFVRAAATCAGLGYLWLRGSVIEDKCREALLLTDKLQNLPFNLAVRQALVL
LVELTLATVGPLLSALFGAVILAALLANRGFVFSLEPMKPNFDKINPFQWLKRLGSARSAVEVGKTLFKVLVLGGTFSLF
FLGLWKTMVYLPVCGMGCFGVVFTGAKQLIGIGAGALLIGGLIDLLLQRALFLREMRMTKTEIKRELKEQQGTPELKGER
RRIRNEMASEPPLGVHRATLVYRGTAVLIGLRYVRGETGVPILVCRAEGEAASDMFREAQNLRLKIVDDHVLAHQLMSTT
KLGTAIPMQYFEPIARALLAAGLA

Specific function: Could be involved in the secretion of an unknown factor

COG id: COG4792

COG function: function code U; Type III secretory pathway, component EscU

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family

Homologues:

Organism=Escherichia coli, GI1788188, Length=359, Percent_Identity=26.4623955431755, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): Y4YO_RHISN (P55723)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444167.1
- ProteinModelPortal:   P55723
- GeneID:   962524
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a00580
- HOGENOM:   HBG655236
- ProtClustDB:   CLSK506583
- InterPro:   IPR006135
- PRINTS:   PR00950

Pfam domain/function: PF01312 Bac_export_2

EC number: NA

Molecular weight: Translated: 37762; Mature: 37631

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb008f68)-; HASH(0xbb18dd0)-; HASH(0xbb19058)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSEEKSHGATPKKLSDARKRGQIPRSSDFVRAAATCAGLGYLWLRGSVIEDKCREAL
CCCCCCCHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLTDKLQNLPFNLAVRQALVLLVELTLATVGPLLSALFGAVILAALLANRGFVFSLEPMK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PNFDKINPFQWLKRLGSARSAVEVGKTLFKVLVLGGTFSLFFLGLWKTMVYLPVCGMGCF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
GVVFTGAKQLIGIGAGALLIGGLIDLLLQRALFLREMRMTKTEIKRELKEQQGTPELKGE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RRRIRNEMASEPPLGVHRATLVYRGTAVLIGLRYVRGETGVPILVCRAEGEAASDMFREA
HHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
QNLRLKIVDDHVLAHQLMSTTKLGTAIPMQYFEPIARALLAAGLA
HCCEEEEEHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SDTSEEKSHGATPKKLSDARKRGQIPRSSDFVRAAATCAGLGYLWLRGSVIEDKCREAL
CCCCCCHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLTDKLQNLPFNLAVRQALVLLVELTLATVGPLLSALFGAVILAALLANRGFVFSLEPMK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PNFDKINPFQWLKRLGSARSAVEVGKTLFKVLVLGGTFSLFFLGLWKTMVYLPVCGMGCF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
GVVFTGAKQLIGIGAGALLIGGLIDLLLQRALFLREMRMTKTEIKRELKEQQGTPELKGE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RRRIRNEMASEPPLGVHRATLVYRGTAVLIGLRYVRGETGVPILVCRAEGEAASDMFREA
HHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
QNLRLKIVDDHVLAHQLMSTTKLGTAIPMQYFEPIARALLAAGLA
HCCEEEEEHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424