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Identifier: 16519958

GI number: 16519958

Start: 179807

End: 180979

Strand: Reverse

Name: Not Available

Synonym: NGR_a01470

Alternate gene names: 16519958

Gene position: 180979-179807 (Counterclockwise)

Preceding gene: 16519957

Following gene: 16519959

Centisome position: 33.75

GC content: 54.39

Gene sequence:

>1173_bases
ATGCCGGTTATCAAGGGACCCCAAGTATTTCCTCGCGCCGAATTCCTTAGGCGACTCAATGCTGTGAAGCTGGAGATGGG
CCGGCTTGAAATCGATGCGCTCATTGTCGGCAGTTCTGCTGATATCACGTATCTGACCGGCTATACGGCTAAGTCCGGAT
ATGTGCCACAGGCTCTCGTAATCTCATCGAATGACGAAGAGCCCACATTCATCCTGCGGCGCCAAGACGCGCCTGCGGCG
ATCCACCAGACATTCATGGATCGGGACAAAGTTATCGGGTACTCTGAAGCCCTCATCGGGAACCCCGATAAGGACGGCTA
TGACGCCGTAGTTGACTTCTTGAACGAACGCGATGGTGCCAACAAGCGCGGTGTTGGAGTGCAGCTTGGCTATCTGTCCG
TAAGATCAGCTGAAAAGTTTAAGACGCGCCTGCCAAGCGCGCGAATTGTTGACTGCACCCATTCGGTCGCGTGGATCCGC
ATGATCAAATCCGATCTCGAAATTTCGATGATGCGTGATGCGGCCGCAATCGCAGACGCAGGAGTCCAAAAGGCATTCGA
AGTCATTCGACCGGGAGTGCGCGAAGCCGACGTTATGGCTGACGTCGCAGCTCAACTTGCGCGGGGGACAAACGGTAAAG
CGGGAACAGATCTCGCGTCGATGTATTTCTGTTCTTCACCCCGAACCGGCACATGCCACATTCGATGGAGTGAAGATGTC
ATCCGCGATGGTTCTCAGATCAATCTTGAAATCGCGGGCGTTCGTCACGGCTATGTCTCAGCAATAATGCGGACTTTCTC
CGTAGGTGCCCCATCCGATCGACTTCGACGCATTCATGACGCGGAGGTACTGGGTTTGGAGGCCGCGCTCAGTACGGTAA
AGCCTGGCGCTACTTGTAGCGACGTCGCAAACGCATTCTACCGCACGATCGAAAAGTCCGGCTTTCAAAAGGACTCCCGC
TGCGGATATGCGATCGGGATCGACTGGAGCGAACCAACTGCAAGCCTGAAGGATGGTGATATGACCAAACTCAAGCCAAA
TATGACCTTCCACCTGATGCTCGGCAATTGGATTGAAGAAGATTTTGGCTACGTACTCAGTGAGACCTTCCGTGTCACCG
AAGCCGGCTGCGAAGTGCTGACGAAGTCGCCGCGGCAACTGTGTGTGATCTAA

Upstream 100 bases:

>100_bases
GGCGCTGACTTCTCATCTGTTCACACGTTACCTTAGTATCCGCCGTCCTGCGGACAATCATGCAACTAAATGCTCAGACC
ATATCCTACAGGAGGCAGCC

Downstream 100 bases:

>100_bases
AGAAGGCAGCCAACGAAAGTTGGTTTCTAACAACGTGACCCACTTCGATAATCAGACGCAAATTGGCTGGTTCGGCCGCG
GAGAAGCGTCAATTCTCAAA

Product: hydrolase/peptidase of the M24 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPVIKGPQVFPRAEFLRRLNAVKLEMGRLEIDALIVGSSADITYLTGYTAKSGYVPQALVISSNDEEPTFILRRQDAPAA
IHQTFMDRDKVIGYSEALIGNPDKDGYDAVVDFLNERDGANKRGVGVQLGYLSVRSAEKFKTRLPSARIVDCTHSVAWIR
MIKSDLEISMMRDAAAIADAGVQKAFEVIRPGVREADVMADVAAQLARGTNGKAGTDLASMYFCSSPRTGTCHIRWSEDV
IRDGSQINLEIAGVRHGYVSAIMRTFSVGAPSDRLRRIHDAEVLGLEAALSTVKPGATCSDVANAFYRTIEKSGFQKDSR
CGYAIGIDWSEPTASLKDGDMTKLKPNMTFHLMLGNWIEEDFGYVLSETFRVTEAGCEVLTKSPRQLCVI

Sequences:

>Translated_390_residues
MPVIKGPQVFPRAEFLRRLNAVKLEMGRLEIDALIVGSSADITYLTGYTAKSGYVPQALVISSNDEEPTFILRRQDAPAA
IHQTFMDRDKVIGYSEALIGNPDKDGYDAVVDFLNERDGANKRGVGVQLGYLSVRSAEKFKTRLPSARIVDCTHSVAWIR
MIKSDLEISMMRDAAAIADAGVQKAFEVIRPGVREADVMADVAAQLARGTNGKAGTDLASMYFCSSPRTGTCHIRWSEDV
IRDGSQINLEIAGVRHGYVSAIMRTFSVGAPSDRLRRIHDAEVLGLEAALSTVKPGATCSDVANAFYRTIEKSGFQKDSR
CGYAIGIDWSEPTASLKDGDMTKLKPNMTFHLMLGNWIEEDFGYVLSETFRVTEAGCEVLTKSPRQLCVI
>Mature_389_residues
PVIKGPQVFPRAEFLRRLNAVKLEMGRLEIDALIVGSSADITYLTGYTAKSGYVPQALVISSNDEEPTFILRRQDAPAAI
HQTFMDRDKVIGYSEALIGNPDKDGYDAVVDFLNERDGANKRGVGVQLGYLSVRSAEKFKTRLPSARIVDCTHSVAWIRM
IKSDLEISMMRDAAAIADAGVQKAFEVIRPGVREADVMADVAAQLARGTNGKAGTDLASMYFCSSPRTGTCHIRWSEDVI
RDGSQINLEIAGVRHGYVSAIMRTFSVGAPSDRLRRIHDAEVLGLEAALSTVKPGATCSDVANAFYRTIEKSGFQKDSRC
GYAIGIDWSEPTASLKDGDMTKLKPNMTFHLMLGNWIEEDFGYVLSETFRVTEAGCEVLTKSPRQLCVI

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family

Homologues:

Organism=Escherichia coli, GI1788728, Length=273, Percent_Identity=26.7399267399267, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786364, Length=248, Percent_Identity=26.6129032258064, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4TL_RHISN (P55666)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444078.1
- ProteinModelPortal:   P55666
- SMR:   P55666
- GeneID:   962224
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01470
- HOGENOM:   HBG537301
- ProtClustDB:   CLSK506520
- InterPro:   IPR000587
- InterPro:   IPR000994
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: 3.4.-.- [C]

Molecular weight: Translated: 42774; Mature: 42643

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIKGPQVFPRAEFLRRLNAVKLEMGRLEIDALIVGSSADITYLTGYTAKSGYVPQALV
CCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEEE
ISSNDEEPTFILRRQDAPAAIHQTFMDRDKVIGYSEALIGNPDKDGYDAVVDFLNERDGA
EECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCCC
NKRGVGVQLGYLSVRSAEKFKTRLPSARIVDCTHSVAWIRMIKSDLEISMMRDAAAIADA
CCCCCEEEEEEEECCCHHHHHHHCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GVQKAFEVIRPGVREADVMADVAAQLARGTNGKAGTDLASMYFCSSPRTGTCHIRWSEDV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCEEEEEECHHH
IRDGSQINLEIAGVRHGYVSAIMRTFSVGAPSDRLRRIHDAEVLGLEAALSTVKPGATCS
HCCCCEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVANAFYRTIEKSGFQKDSRCGYAIGIDWSEPTASLKDGDMTKLKPNMTFHLMLGNWIEE
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHH
DFGYVLSETFRVTEAGCEVLTKSPRQLCVI
HHHHHHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
PVIKGPQVFPRAEFLRRLNAVKLEMGRLEIDALIVGSSADITYLTGYTAKSGYVPQALV
CCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEEE
ISSNDEEPTFILRRQDAPAAIHQTFMDRDKVIGYSEALIGNPDKDGYDAVVDFLNERDGA
EECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCCC
NKRGVGVQLGYLSVRSAEKFKTRLPSARIVDCTHSVAWIRMIKSDLEISMMRDAAAIADA
CCCCCEEEEEEEECCCHHHHHHHCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GVQKAFEVIRPGVREADVMADVAAQLARGTNGKAGTDLASMYFCSSPRTGTCHIRWSEDV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCEEEEEECHHH
IRDGSQINLEIAGVRHGYVSAIMRTFSVGAPSDRLRRIHDAEVLGLEAALSTVKPGATCS
HCCCCEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DVANAFYRTIEKSGFQKDSRCGYAIGIDWSEPTASLKDGDMTKLKPNMTFHLMLGNWIEE
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHH
DFGYVLSETFRVTEAGCEVLTKSPRQLCVI
HHHHHHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424