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Identifier: 16519959

GI number: 16519959

Start: 178466

End: 179644

Strand: Reverse

Name: Not Available

Synonym: NGR_a01460

Alternate gene names: 16519959

Gene position: 179644-178466 (Counterclockwise)

Preceding gene: 16519958

Following gene: 16519960

Centisome position: 33.51

GC content: 52.84

Gene sequence:

>1179_bases
ATGCGTATTCTTCATTTCTCGGACGCTGAGTATCAGCAGCGGATGCTCCGAGTAAAACAGCGGATGCAAGCTCAAGACAT
CGATGTCTTGATTGTCACTGAGCCGGCCAACATCAACTATCTCACTGGATACGATGCTTGGTCCTTCTATACGGTTCAGG
CACTGTTGGTGTTCCAAGACGTTGATTTACCGATGTGGATTGGCAGATCAATAGATAAGCAATCCGCAATTGTTACAACA
AATCTTCCTGATGAACGGATTATACCATATCCGGACATATATGTTCATTCCCCAGATCGGCACGCCGCGCAATTTATTGC
GCAAATGCTGTTAAGGGAGGCTCCGCGTGCCAAGACTGTCGGCGTGGAAATGGGCGCTTATTATTATACAGCGCGTGATC
ATGCTGAACTGCAGAAGGCATTGCCAAATGTCGCGTTCAAGGACGCAGAGTTGCTCGTAAATTGGGTGCGCTTCATCAAG
AGCGAGCGGGAAATCGCTTACATGCGAGAAGCCGGCGTGATTACTGAACGCATGATGAAACGGGCGGTAGAAGTCGCAGC
GCCAGGTGTACGACAGTGTGACGTGGCCGCTGCAATCTACCATGCACAAATGTCGGGCACAGAGAACTATGGCGGGCTTC
CTGCCACCAGCCCACCACACATGGGGTTCGGTGAGCGCGCTAGGGAGCCGCACCCGATATTTACCGATCGACGTATCGAC
ACAAATTCTGTTGCCAATATCGAATTGTCGGGATGCCGACTTCGATATCACGCACCCATGAGCAGGACAGTCTATTTCGG
CAGGCCGCCCCAGAGTTACCGGGACTTAGCGTCCTATGTGATTGAGGCCGTCGAAACGTCACTCGATGCTGTCCGACCTG
GTGTGACGTGCGAAGGCATCGAATTAGCGTGGCGCAAGAGCATGAGCGCTCATGGCATCGAGAAGGAAAACCGGCTGGGT
TACTCCATTGGCATTGCCTACACACCGACATGGGGCGAGCGAACGGCGAGCATAAGGCGGACGGATCTTACTGTTCTTGA
GCCTGGGGCGGCATTTCACCTCATGGGTGGATTGTGGTTGGCAAACACCGGAATAACGATCACGCAGTCTTTCGTTGTAA
CAGCAACGGGTCACGAACCCCTTACGGCTACGCCTCGGGAACTGGTCGTTAAAGACTGA

Upstream 100 bases:

>100_bases
TTGGCTGGTTCGGCCGCGGAGAAGCGTCAATTCTCAAACCGTCAGCAGGGAGCCTTAGCGTCGTTGGACGCGAGCGCTCT
CGAAGCGTCAAGGAACCAAA

Downstream 100 bases:

>100_bases
CGCCAGATGGGCCCGCCGAGGCTATGAGCACTTCAACGCGACGTGTAGTCCGATTTCGACTTTCGTTTGCGTCGCAGCCG
TCGCCTGTATGCGTTTTTTT

Product: hydrolase/peptidase of the M24 family

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRILHFSDAEYQQRMLRVKQRMQAQDIDVLIVTEPANINYLTGYDAWSFYTVQALLVFQDVDLPMWIGRSIDKQSAIVTT
NLPDERIIPYPDIYVHSPDRHAAQFIAQMLLREAPRAKTVGVEMGAYYYTARDHAELQKALPNVAFKDAELLVNWVRFIK
SEREIAYMREAGVITERMMKRAVEVAAPGVRQCDVAAAIYHAQMSGTENYGGLPATSPPHMGFGERAREPHPIFTDRRID
TNSVANIELSGCRLRYHAPMSRTVYFGRPPQSYRDLASYVIEAVETSLDAVRPGVTCEGIELAWRKSMSAHGIEKENRLG
YSIGIAYTPTWGERTASIRRTDLTVLEPGAAFHLMGGLWLANTGITITQSFVVTATGHEPLTATPRELVVKD

Sequences:

>Translated_392_residues
MRILHFSDAEYQQRMLRVKQRMQAQDIDVLIVTEPANINYLTGYDAWSFYTVQALLVFQDVDLPMWIGRSIDKQSAIVTT
NLPDERIIPYPDIYVHSPDRHAAQFIAQMLLREAPRAKTVGVEMGAYYYTARDHAELQKALPNVAFKDAELLVNWVRFIK
SEREIAYMREAGVITERMMKRAVEVAAPGVRQCDVAAAIYHAQMSGTENYGGLPATSPPHMGFGERAREPHPIFTDRRID
TNSVANIELSGCRLRYHAPMSRTVYFGRPPQSYRDLASYVIEAVETSLDAVRPGVTCEGIELAWRKSMSAHGIEKENRLG
YSIGIAYTPTWGERTASIRRTDLTVLEPGAAFHLMGGLWLANTGITITQSFVVTATGHEPLTATPRELVVKD
>Mature_392_residues
MRILHFSDAEYQQRMLRVKQRMQAQDIDVLIVTEPANINYLTGYDAWSFYTVQALLVFQDVDLPMWIGRSIDKQSAIVTT
NLPDERIIPYPDIYVHSPDRHAAQFIAQMLLREAPRAKTVGVEMGAYYYTARDHAELQKALPNVAFKDAELLVNWVRFIK
SEREIAYMREAGVITERMMKRAVEVAAPGVRQCDVAAAIYHAQMSGTENYGGLPATSPPHMGFGERAREPHPIFTDRRID
TNSVANIELSGCRLRYHAPMSRTVYFGRPPQSYRDLASYVIEAVETSLDAVRPGVTCEGIELAWRKSMSAHGIEKENRLG
YSIGIAYTPTWGERTASIRRTDLTVLEPGAAFHLMGGLWLANTGITITQSFVVTATGHEPLTATPRELVVKD

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4TM_RHISN (P55667)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444079.1
- ProteinModelPortal:   P55667
- GeneID:   962449
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01460
- HOGENOM:   HBG537301
- ProtClustDB:   CLSK864646
- InterPro:   IPR000587
- InterPro:   IPR000994
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 44094; Mature: 44094

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHFSDAEYQQRMLRVKQRMQAQDIDVLIVTEPANINYLTGYDAWSFYTVQALLVFQD
CEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHEEEEEEEC
VDLPMWIGRSIDKQSAIVTTNLPDERIIPYPDIYVHSPDRHAAQFIAQMLLREAPRAKTV
CCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEE
GVEMGAYYYTARDHAELQKALPNVAFKDAELLVNWVRFIKSEREIAYMREAGVITERMMK
EEEECEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RAVEVAAPGVRQCDVAAAIYHAQMSGTENYGGLPATSPPHMGFGERAREPHPIFTDRRID
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
TNSVANIELSGCRLRYHAPMSRTVYFGRPPQSYRDLASYVIEAVETSLDAVRPGVTCEGI
CCCEEEEEECCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELAWRKSMSAHGIEKENRLGYSIGIAYTPTWGERTASIRRTDLTVLEPGAAFHLMGGLWL
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCEEEECCCCCEEHHCCEEE
ANTGITITQSFVVTATGHEPLTATPRELVVKD
ECCCCEEEEEEEEEECCCCCCCCCCHHHEECC
>Mature Secondary Structure
MRILHFSDAEYQQRMLRVKQRMQAQDIDVLIVTEPANINYLTGYDAWSFYTVQALLVFQD
CEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHEEEEEEEC
VDLPMWIGRSIDKQSAIVTTNLPDERIIPYPDIYVHSPDRHAAQFIAQMLLREAPRAKTV
CCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEE
GVEMGAYYYTARDHAELQKALPNVAFKDAELLVNWVRFIKSEREIAYMREAGVITERMMK
EEEECEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RAVEVAAPGVRQCDVAAAIYHAQMSGTENYGGLPATSPPHMGFGERAREPHPIFTDRRID
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
TNSVANIELSGCRLRYHAPMSRTVYFGRPPQSYRDLASYVIEAVETSLDAVRPGVTCEGI
CCCEEEEEECCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELAWRKSMSAHGIEKENRLGYSIGIAYTPTWGERTASIRRTDLTVLEPGAAFHLMGGLWL
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCEEEECCCCCEEHHCCEEE
ANTGITITQSFVVTATGHEPLTATPRELVVKD
ECCCCEEEEEEEEEECCCCCCCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424