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Identifier: 16519895

GI number: 16519895

Start: 275950

End: 276747

Strand: Reverse

Name: Not Available

Synonym: NGR_a02100

Alternate gene names: 16519895

Gene position: 276747-275950 (Counterclockwise)

Preceding gene: 16519894

Following gene: 16519896

Centisome position: 51.62

GC content: 62.28

Gene sequence:

>798_bases
ATGTCATGCCAGACGCAGCTTCTCAGCGCCTCGGAGGCCGCCTTAGGAGAGGACTTCCTCTCCGCTCTCTCGACGGATCT
GGACAATTGGGCTCTGTTCCTCGATATCGATGGTACTTTGCTTGACCTGGCCGAAACGCCGGACGCCGTAGCCGTTCCGC
CTTCGCTGCCTGCAAGTCTCGATCACTTGTCGAAAAAACTCGGCGGCGCTTTGGCACTCGTGACCGGTCGCGGCCTTGAT
TATGCCGACCAGCTTTTTTCGCCGGCCAATTTTCCCATTGCGGGCCTTCATGGTGCCGAGCGCCGCGACCCGGATGGCCG
TGTGCACAAAGCCGCAGAGACGGCGGATTTTGAGCGACTGAAGGCCGAGCTGGTCGCCGCTACTGCCAGCTGGGCGGGCG
TTCTGATCGAGGACAAGGGGGCGGCGGTCGCTGCCCATTATCGGCTTGCGCCGGATAGACAACTCGAACTTGAACAGCTG
ATGGAGTGGGCGTTGTATCGAGCCGGACCAGACTGGGCAATCCAGCACGGCAAGATGGTCGTCGAAATCCGTCCAGCCAG
AGCCAACAAGGGCGATGCAGTTGCGGCATTTCTCGGCCAGCCGCCCTTTGCCGGCAGACGCGCGATCGCGATCGGCGACG
ATGTGACCGACGAAGCGATGTTTCGCACGGTCAACCGGCTCGGCGGCCTTTCGATCCGCATCGGACCGCCTGTGCCTGCG
AGCGAAGCGCTCGGCTCCATCCCTTCTGCCGAGGCCCTGCGCGGCATCATCGCCGCATTGGCCCTCCTGAATATTTGA

Upstream 100 bases:

>100_bases
TGACTGTCCAAAGGAAGTGCTGCGCCTCTCAACTCGCCATCTGACTTTAAATGGATGGCAGGGGTGGCCGGTAACGGGCA
TCTGGTATAGACATGGCGTC

Downstream 100 bases:

>100_bases
GCACCTGACATATTTTCTTGCATGAAAGGAATTCGGCATGAGCCGTCTCGTCATTGTTTCCAATCGCGTACCTGTTCCGG
ACAAGGGTGGCATTGCGCCG

Product: trehalose 6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSCQTQLLSASEAALGEDFLSALSTDLDNWALFLDIDGTLLDLAETPDAVAVPPSLPASLDHLSKKLGGALALVTGRGLD
YADQLFSPANFPIAGLHGAERRDPDGRVHKAAETADFERLKAELVAATASWAGVLIEDKGAAVAAHYRLAPDRQLELEQL
MEWALYRAGPDWAIQHGKMVVEIRPARANKGDAVAAFLGQPPFAGRRAIAIGDDVTDEAMFRTVNRLGGLSIRIGPPVPA
SEALGSIPSAEALRGIIAALALLNI

Sequences:

>Translated_265_residues
MSCQTQLLSASEAALGEDFLSALSTDLDNWALFLDIDGTLLDLAETPDAVAVPPSLPASLDHLSKKLGGALALVTGRGLD
YADQLFSPANFPIAGLHGAERRDPDGRVHKAAETADFERLKAELVAATASWAGVLIEDKGAAVAAHYRLAPDRQLELEQL
MEWALYRAGPDWAIQHGKMVVEIRPARANKGDAVAAFLGQPPFAGRRAIAIGDDVTDEAMFRTVNRLGGLSIRIGPPVPA
SEALGSIPSAEALRGIIAALALLNI
>Mature_264_residues
SCQTQLLSASEAALGEDFLSALSTDLDNWALFLDIDGTLLDLAETPDAVAVPPSLPASLDHLSKKLGGALALVTGRGLDY
ADQLFSPANFPIAGLHGAERRDPDGRVHKAAETADFERLKAELVAATASWAGVLIEDKGAAVAAHYRLAPDRQLELEQLM
EWALYRAGPDWAIQHGKMVVEIRPARANKGDAVAAFLGQPPFAGRRAIAIGDDVTDEAMFRTVNRLGGLSIRIGPPVPAS
EALGSIPSAEALRGIIAALALLNI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family

Homologues:

Organism=Escherichia coli, GI1788207, Length=208, Percent_Identity=42.7884615384615, Blast_Score=164, Evalue=8e-42,
Organism=Drosophila melanogaster, GI221473368, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129307, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582490, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920676, Length=202, Percent_Identity=28.2178217821782, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSB_RHISN (P55611)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_444015.1
- ProteinModelPortal:   P55611
- SMR:   P55611
- GeneID:   962403
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a02100
- HOGENOM:   HBG686849
- ProtClustDB:   CLSK809012
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484
- TIGRFAMs:   TIGR00685

Pfam domain/function: PF02358 Trehalose_PPase; SSF56784 SSF56784

EC number: =3.1.3.12

Molecular weight: Translated: 27958; Mature: 27827

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCQTQLLSASEAALGEDFLSALSTDLDNWALFLDIDGTLLDLAETPDAVAVPPSLPASL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEECCCCCCHHH
DHLSKKLGGALALVTGRGLDYADQLFSPANFPIAGLHGAERRDPDGRVHKAAETADFERL
HHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
KAELVAATASWAGVLIEDKGAAVAAHYRLAPDRQLELEQLMEWALYRAGPDWAIQHGKMV
HHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCEEE
VEIRPARANKGDAVAAFLGQPPFAGRRAIAIGDDVTDEAMFRTVNRLGGLSIRIGPPVPA
EEEECCCCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCH
SEALGSIPSAEALRGIIAALALLNI
HHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCQTQLLSASEAALGEDFLSALSTDLDNWALFLDIDGTLLDLAETPDAVAVPPSLPASL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEECCCCCCHHH
DHLSKKLGGALALVTGRGLDYADQLFSPANFPIAGLHGAERRDPDGRVHKAAETADFERL
HHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
KAELVAATASWAGVLIEDKGAAVAAHYRLAPDRQLELEQLMEWALYRAGPDWAIQHGKMV
HHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCEEE
VEIRPARANKGDAVAAFLGQPPFAGRRAIAIGDDVTDEAMFRTVNRLGGLSIRIGPPVPA
EEEECCCCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCH
SEALGSIPSAEALRGIIAALALLNI
HHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424