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Identifier: 16519861

GI number: 16519861

Start: 322128

End: 322967

Strand: Direct

Name: Not Available

Synonym: NGR_a02440

Alternate gene names: 16519861

Gene position: 322128-322967 (Clockwise)

Preceding gene: 16519862

Following gene: 16519860

Centisome position: 60.08

GC content: 62.62

Gene sequence:

>840_bases
ATGGCTCAGATCGGTCACAACATCAGTCTGCCCGAGCGTGCGCGCCGCATTCGCCGGCATGCGCTCCGCATGGGCGAAGT
TCAGGGCCAGGGCTATATCGCACAGGCGCTCGGCATCGCAGACGTTCTCGCGGTCGCCTATTTCCATGCGACGACCTACC
GGCCGGACGATCCGGAGTGGGAAGGTCGCGACCGTTTCCTATTGTCGATCGGCCATTATGCCATCGCACTCTATGCGGCC
TTGATCGAGGCGAAGATCATTCCCGAAGATGAACTGGAAACCTATGGCGCCGATGACAGCCGCCTGCCGATGTCCGGCAT
GGCGGCCTACACGCCGGGCATGGAGATTACCGGAGGATCGCTGGGTCACGGCCTCGGCATAGCCGTCGGCATGTCGCTCG
CGCTGAAGCGCAAAGGTTCGCGGTCCTTCGTCTACAACCTCTTCTCCGACGGTGAGCTCGACGAGGGTTCTACCTGGGAA
GCGGCCATGTCGGCCGGTTCCTACAAGCTCGACAACCTGATCGGCATCGTCGACGTCAATCAGATGCAGGCGGACGGCCC
GTCGCTCGGGGTGCTCAACTTCGAGCCTCTCGGTCCAAAGTTCGAGGCCTTCGGCTGGTACGTCCAACGCATCGACGGCA
ACGACATCGATGCGCTCGTCGACGCTTTCGACAATGCGCGCCAGCACCGGCATCCGCAGCCCAGAATCATCATCTGCGAT
ACGAAGATGGCCAAGGGCGTGCCCTTCCTGGAGGCGCGTGAGCGCAACCACTTCCTGCGCGTCGAACCGCAGGAATGGGC
CGAGGCGATCCGGATCATCGACGCGGGAGTGACGGCATGA

Upstream 100 bases:

>100_bases
CGCATCCGATCTCTCCGCCTACGTCACGGGCGCCGTCATCGACGTCAATGGCGGCATGCTGATCCATTGATCCACGAGGA
AGCCTGAAAGGAAGGACGGC

Downstream 100 bases:

>100_bases
GACGCTCGAAATACGAACGCCCCGCCCATCTGATCGGCAATGCGGAGAGACCCCGATTAACCACGTCGGCGATGATCGCT
TCCATTGCCGGCGCCGACCA

Product: transketolase alpha-subunit protein

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAQIGHNISLPERARRIRRHALRMGEVQGQGYIAQALGIADVLAVAYFHATTYRPDDPEWEGRDRFLLSIGHYAIALYAA
LIEAKIIPEDELETYGADDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMSLALKRKGSRSFVYNLFSDGELDEGSTWE
AAMSAGSYKLDNLIGIVDVNQMQADGPSLGVLNFEPLGPKFEAFGWYVQRIDGNDIDALVDAFDNARQHRHPQPRIIICD
TKMAKGVPFLEARERNHFLRVEPQEWAEAIRIIDAGVTA

Sequences:

>Translated_279_residues
MAQIGHNISLPERARRIRRHALRMGEVQGQGYIAQALGIADVLAVAYFHATTYRPDDPEWEGRDRFLLSIGHYAIALYAA
LIEAKIIPEDELETYGADDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMSLALKRKGSRSFVYNLFSDGELDEGSTWE
AAMSAGSYKLDNLIGIVDVNQMQADGPSLGVLNFEPLGPKFEAFGWYVQRIDGNDIDALVDAFDNARQHRHPQPRIIICD
TKMAKGVPFLEARERNHFLRVEPQEWAEAIRIIDAGVTA
>Mature_278_residues
AQIGHNISLPERARRIRRHALRMGEVQGQGYIAQALGIADVLAVAYFHATTYRPDDPEWEGRDRFLLSIGHYAIALYAAL
IEAKIIPEDELETYGADDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMSLALKRKGSRSFVYNLFSDGELDEGSTWEA
AMSAGSYKLDNLIGIVDVNQMQADGPSLGVLNFEPLGPKFEAFGWYVQRIDGNDIDALVDAFDNARQHRHPQPRIIICDT
KMAKGVPFLEARERNHFLRVEPQEWAEAIRIIDAGVTA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family

Homologues:

Organism=Homo sapiens, GI133778974, Length=273, Percent_Identity=36.6300366300366, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI205277463, Length=266, Percent_Identity=35.7142857142857, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4507521, Length=266, Percent_Identity=35.7142857142857, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI225637459, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI225637461, Length=264, Percent_Identity=30.3030303030303, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI225637463, Length=231, Percent_Identity=32.034632034632, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1788808, Length=264, Percent_Identity=34.0909090909091, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI48994911, Length=254, Percent_Identity=33.0708661417323, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17539652, Length=267, Percent_Identity=33.7078651685393, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325331, Length=245, Percent_Identity=31.8367346938775, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319593, Length=243, Percent_Identity=29.6296296296296, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45551847, Length=253, Percent_Identity=32.0158102766798, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550715, Length=253, Percent_Identity=32.0158102766798, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24666278, Length=253, Percent_Identity=29.6442687747036, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=33.6363636363636, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y4MO_RHISN (P55574)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443981.1
- ProteinModelPortal:   P55574
- SMR:   P55574
- GeneID:   962368
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a02440
- HOGENOM:   HBG670382
- ProtClustDB:   CLSK864385
- InterPro:   IPR005474

Pfam domain/function: PF00456 Transketolase_N

EC number: 2.2.1.1

Molecular weight: Translated: 30743; Mature: 30612

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00801 TRANSKETOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x4039b7d8)-; HASH(0xbd4a8b4)-; HASH(0x9618db0)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIGHNISLPERARRIRRHALRMGEVQGQGYIAQALGIADVLAVAYFHATTYRPDDPEW
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAKIIPEDELETYGADDSRLPMSGMAAYTPGMEITGGS
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMSLALKRKGSRSFVYNLFSDGELDEGSTWEAAMSAGSYKLDNLIGIVDVN
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCEECCCEEEEEECC
QMQADGPSLGVLNFEPLGPKFEAFGWYVQRIDGNDIDALVDAFDNARQHRHPQPRIIICD
HHCCCCCCEEEEECCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TKMAKGVPFLEARERNHFLRVEPQEWAEAIRIIDAGVTA
CHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
AQIGHNISLPERARRIRRHALRMGEVQGQGYIAQALGIADVLAVAYFHATTYRPDDPEW
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAKIIPEDELETYGADDSRLPMSGMAAYTPGMEITGGS
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMSLALKRKGSRSFVYNLFSDGELDEGSTWEAAMSAGSYKLDNLIGIVDVN
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCEECCCEEEEEECC
QMQADGPSLGVLNFEPLGPKFEAFGWYVQRIDGNDIDALVDAFDNARQHRHPQPRIIICD
HHCCCCCCEEEEECCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TKMAKGVPFLEARERNHFLRVEPQEWAEAIRIIDAGVTA
CHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424