BacMap

Definition	Sinorhizobium fredii NGR234 plasmid pNGR234a, complete sequence.
Accession	NC_000914
Length	536,165

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The map label for this gene is Not Available

Identifier: 16519832

GI number: 16519832

Start: 361252

End: 362088

Strand: Direct

Name: Not Available

Synonym: NGR_a02730

Alternate gene names: 16519832

Gene position: 361252-362088 (Clockwise)

Preceding gene: 16519833

Following gene: 16519831

Centisome position: 67.38

GC content: 68.94

Gene sequence:

>837_bases
ATGGAACGGTTTGAAGGCAAGGTGGCCGTGGTGACCGGCGCCGGCGCCGGCATCGGCAAGGCATGTGCCCTCGCCATCGC
GCGCGAGGGCGGCAGAGTGGTGGTGGCCGACCTTGATGGCTCGGCGGCCATCGCCTGCACCGCGCAGATCGCGGCCGAAG
CGGGCAATGCGCTGGCCATGGCCATGGACATCGCCGATGCGCAGGCAGTGGCAGCGCTGTTCGAGACGGCGGAGCGGCAC
TTCGGTGGGGTCGACCTGCTGGTGAACAACGCGAGCGCCATGCATCTGACCCCGCGCGACCGCGCGATCCTCGACCTGGA
CCTGGCGGTCTGGGATCAGACCATGGCGACCAATCTGCGCGGCACGCTGCTCTGCTGCCGACAGGCCATCCCACGGATGA
TCGCCCGCGGCGGTGGCGCGATCGTCAACATGTCGTCGTGCCAGGGGCTCAGCGGTGACACCGCGCAGACGTCATACGCC
GTGTCGAAGGCGGCGATGAACATGCTGTCGGCCTCGCTCGCCACCCAGTACGGCCATGCGCAGATCCGCTGCAACGCGGT
TGCGCCGGGTCTCATCATGACCGAGCGTCTCCTGGCCAAGCTGGACAAGTGCATGCAACGGCATCTCAGCCGGCACCAGC
TCCTGCCGCGCGTCGGCCACCCCGAGGACGTGGCCGCGCTGGTGGCGTTCCTGCTCTCCGACGATGCTTCGTTCATCACC
GGCCAGGTCGTGTGCATCGACGGCGGCATGCTGGCGCATATGCCGACGTACGCCGACGGTGGCAACAGCTGCGCCGGGCG
GACTGCGGGCGACACCGCCGAAACGGCGCGCTGCTGA

Upstream 100 bases:

>100_bases
GACGCTGGCGATGACGAGCGCGCCAGCGCCGACCCTGCGCGTTCTCCGGCGGAGGCCGAGCGGCATGCGGCGAAAGACCA
ACGCATTCCAGGAGGACACG

Downstream 100 bases:

>100_bases
TGAACGTGCTGCTCAACCCCCTAAACCGTCGGCACCGGCTGCGGTACGACATCCCCGTCATGCCCGGCGCTTTCCCCCTG
GTCGGGCATCTTCCCGCCAT

Product: short chain dehydrogenase/reductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAAEAGNALAMAMDIADAQAVAALFETAERH
FGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYA
VSKAAMNMLSASLATQYGHAQIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPEDVAALVAFLLSDDASFIT
GQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC

Sequences:

>Translated_278_residues
MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAAEAGNALAMAMDIADAQAVAALFETAERH
FGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYA
VSKAAMNMLSASLATQYGHAQIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPEDVAALVAFLLSDDASFIT
GQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC
>Mature_278_residues
MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAAEAGNALAMAMDIADAQAVAALFETAERH
FGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYA
VSKAAMNMLSASLATQYGHAQIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPEDVAALVAFLLSDDASFIT
GQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=34.8, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=34.6007604562738, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=35.3658536585366, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI19923817, Length=276, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5031737, Length=245, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI66933014, Length=255, Percent_Identity=31.7647058823529, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4504505, Length=240, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI126723750, Length=250, Percent_Identity=29.6, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=30.6201550387597, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7705925, Length=260, Percent_Identity=32.6923076923077, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI40254986, Length=197, Percent_Identity=34.010152284264, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI31542939, Length=233, Percent_Identity=29.6137339055794, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4503301, Length=250, Percent_Identity=29.6, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209862901, Length=216, Percent_Identity=31.0185185185185, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI19923983, Length=216, Percent_Identity=31.0185185185185, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI109715829, Length=254, Percent_Identity=26.3779527559055, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI33667109, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=30.2197802197802, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI20149619, Length=194, Percent_Identity=29.3814432989691, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI224922801, Length=164, Percent_Identity=32.9268292682927, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI59889578, Length=252, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4758504, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI40807363, Length=194, Percent_Identity=28.3505154639175, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI302344755, Length=104, Percent_Identity=34.6153846153846, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI157739938, Length=194, Percent_Identity=27.8350515463918, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4502601, Length=146, Percent_Identity=34.2465753424658, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=34.8178137651822, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=32.5490196078431, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=33.7209302325581, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=33.6032388663968, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=27.1255060728745, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789208, Length=253, Percent_Identity=30.8300395256917, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788459, Length=253, Percent_Identity=28.8537549407115, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786812, Length=259, Percent_Identity=32.4324324324324, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1789378, Length=252, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=27.2373540856031, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=29.8850574712644, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787820, Length=187, Percent_Identity=25.1336898395722, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=272, Percent_Identity=31.6176470588235, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=32.8413284132841, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=30.9160305343511, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=33.2075471698113, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=31.5789473684211, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=31.5175097276265, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=30.8, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=31.9548872180451, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=33.0645161290323, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=31.25, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531453, Length=276, Percent_Identity=32.9710144927536, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17560676, Length=250, Percent_Identity=30.4, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25147288, Length=247, Percent_Identity=31.1740890688259, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=30.5660377358491, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536025, Length=286, Percent_Identity=31.4685314685315, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17568967, Length=287, Percent_Identity=30.6620209059233, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=29.0566037735849, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=31.5589353612167, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17536651, Length=291, Percent_Identity=30.9278350515464, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=28.1954887218045, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=30.0380228136882, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17538182, Length=265, Percent_Identity=29.811320754717, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=28.4090909090909, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=30.1587301587302, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=30.9433962264151, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=29.4339622641509, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=27.4809160305344, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17561272, Length=251, Percent_Identity=32.2709163346614, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538486, Length=264, Percent_Identity=28.4090909090909, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=265, Percent_Identity=26.4150943396226, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982365, Length=197, Percent_Identity=29.4416243654822, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558628, Length=196, Percent_Identity=31.6326530612245, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17555612, Length=212, Percent_Identity=30.6603773584906, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=162, Percent_Identity=28.3950617283951, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=159, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=256, Percent_Identity=26.953125, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71997099, Length=208, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322861, Length=262, Percent_Identity=28.6259541984733, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324126, Length=267, Percent_Identity=26.2172284644195, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323882, Length=205, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=34.1269841269841, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355319, Length=260, Percent_Identity=30.7692307692308, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24639444, Length=247, Percent_Identity=31.1740890688259, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357041, Length=261, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571526, Length=266, Percent_Identity=33.4586466165414, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI18859837, Length=252, Percent_Identity=31.7460317460317, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=30.4182509505703, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24584744, Length=204, Percent_Identity=31.3725490196078, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28574402, Length=204, Percent_Identity=31.3725490196078, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17737361, Length=259, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357673, Length=197, Percent_Identity=30.4568527918782, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644337, Length=262, Percent_Identity=30.1526717557252, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=28.4, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161076933, Length=195, Percent_Identity=29.2307692307692, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24584748, Length=195, Percent_Identity=29.2307692307692, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24641232, Length=243, Percent_Identity=27.5720164609054, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358495, Length=255, Percent_Identity=25.4901960784314, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331218, Length=254, Percent_Identity=27.5590551181102, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665243, Length=254, Percent_Identity=27.5590551181102, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665247, Length=255, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221331216, Length=255, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24663911, Length=222, Percent_Identity=28.8288288288288, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355631, Length=170, Percent_Identity=28.8235294117647, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281364494, Length=204, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007234, Length=247, Percent_Identity=28.7449392712551, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648693, Length=212, Percent_Identity=30.6603773584906, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355889, Length=212, Percent_Identity=30.6603773584906, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007236, Length=244, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581726, Length=199, Percent_Identity=28.1407035175879, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28743; Mature: 28743

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAAEAGNALAM
CCCCCCCEEEEEECCCCHHHHHHHHEECCCCEEEEEECCCCEEEEEHHHHHHHCCCEEEE
AMDIADAQAVAALFETAERHFGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLR
EEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHCHH
GTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYAVSKAAMNMLSASLATQYGHA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
QIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPEDVAALVAFLLSDDASFIT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHE
GQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC
EEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAAEAGNALAM
CCCCCCCEEEEEECCCCHHHHHHHHEECCCCEEEEEECCCCEEEEEHHHHHHHCCCEEEE
AMDIADAQAVAALFETAERHFGGVDLLVNNASAMHLTPRDRAILDLDLAVWDQTMATNLR
EEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHCHH
GTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYAVSKAAMNMLSASLATQYGHA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
QIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPEDVAALVAFLLSDDASFIT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHE
GQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC
EEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]