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The map label for this gene is rmlD

Identifier: 16519749

GI number: 16519749

Start: 458417

End: 459307

Strand: Reverse

Name: rmlD

Synonym: NGR_a03570

Alternate gene names: 16519749

Gene position: 459307-458417 (Counterclockwise)

Preceding gene: 16519748

Following gene: 16519750

Centisome position: 85.67

GC content: 57.24

Gene sequence:

>891_bases
ATGCGGCTCGCGGTGACCGGCAAAAACGGACAAATCGCCCTCGCTTTGAAGGCGCAGGCGCGACCTGACGTCGAGATACT
TACTTTGGGGCGGCCGAATTTCGATCTGGCTTGCCGCTCGACTGTCGCAAGCTCCATCAGGGATGCCGCACCGGACATAA
TTGTCTCTTTGGCCGCCTATACCGCCGTCGATAAGGCCGAAAGCGAGCCATACGAAGCCTTCGCTGTGAACCGCGATGGA
GTGCAGGCACTGGCCGAGGCAGCCGCCGGGCTCGGTGTCCCAGTTATTCATCTTTCGACAGATTACGTGTTTGACGGTGC
GAAGCCGGTGCCTTACTGCGAGGAAGACCGCACAGGCCCGATTTCAGTTTATGGCAGGTCCAAACTTGAGGGTGAATTTG
CGGTTGCCTCCGCCAACCCAAATCATACGATCCTGCGCACTTCATGGGTCTATTCTCGATACGGCCAAAACTTCGTGAAG
AAGATGCTCCGGCTCGCCGATACGAATGACGAATTGAATGTCGTCGCCGATCAGCTCGGCTGTCCGACATCGGCTGACGA
CATCAGCGTCGCCGTCATGACGATAGCCAGACGGATGCTGTCGAGCTCATCGGCTGATTTGCGCGGGATATTCCACCTGT
CAGGATCGGGTGAGGCCAGCTGGGCGGCCTTCGCGAAATACGTCTTTTCCGTTTACGACGAGATAACCGGGCGCCAAATC
AAAGTCCATGACATATCCGCTGCAGAATATCCCACACCGGCTCGCCGCCCAGCAAATTCGAGGCTCCACTGCGACAAGCT
TGAGCGAACCTTTGGTATCCGTCTGCCAAATTGGGAGGAGTCCACGCGCCGGCTGGTTTGGGCGCTGCTTTTAGAAGGGA
AAGATGCGTGA

Upstream 100 bases:

>100_bases
AACGGTGGAATGGTATCTGGAAAATGGCTGGTGGTGGCAACCGCTGCGGGACAAGGTTTATTCCGGTGAGCGCCTCGGTC
TCCTGGAGAAAGCGTGACAG

Downstream 100 bases:

>100_bases
AGGGAATTATTCTAGCAGGTGGCAGCGGTACGCGCCTTCACCCGATGACGCTTGTCATGTCAAAGCAAATCTTACCTGTC
TACGACAAGCCGATGATCTT

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRLAVTGKNGQIALALKAQARPDVEILTLGRPNFDLACRSTVASSIRDAAPDIIVSLAAYTAVDKAESEPYEAFAVNRDG
VQALAEAAAGLGVPVIHLSTDYVFDGAKPVPYCEEDRTGPISVYGRSKLEGEFAVASANPNHTILRTSWVYSRYGQNFVK
KMLRLADTNDELNVVADQLGCPTSADDISVAVMTIARRMLSSSSADLRGIFHLSGSGEASWAAFAKYVFSVYDEITGRQI
KVHDISAAEYPTPARRPANSRLHCDKLERTFGIRLPNWEESTRRLVWALLLEGKDA

Sequences:

>Translated_296_residues
MRLAVTGKNGQIALALKAQARPDVEILTLGRPNFDLACRSTVASSIRDAAPDIIVSLAAYTAVDKAESEPYEAFAVNRDG
VQALAEAAAGLGVPVIHLSTDYVFDGAKPVPYCEEDRTGPISVYGRSKLEGEFAVASANPNHTILRTSWVYSRYGQNFVK
KMLRLADTNDELNVVADQLGCPTSADDISVAVMTIARRMLSSSSADLRGIFHLSGSGEASWAAFAKYVFSVYDEITGRQI
KVHDISAAEYPTPARRPANSRLHCDKLERTFGIRLPNWEESTRRLVWALLLEGKDA
>Mature_296_residues
MRLAVTGKNGQIALALKAQARPDVEILTLGRPNFDLACRSTVASSIRDAAPDIIVSLAAYTAVDKAESEPYEAFAVNRDG
VQALAEAAAGLGVPVIHLSTDYVFDGAKPVPYCEEDRTGPISVYGRSKLEGEFAVASANPNHTILRTSWVYSRYGQNFVK
KMLRLADTNDELNVVADQLGCPTSADDISVAVMTIARRMLSSSSADLRGIFHLSGSGEASWAAFAKYVFSVYDEITGRQI
KVHDISAAEYPTPARRPANSRLHCDKLERTFGIRLPNWEESTRRLVWALLLEGKDA

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family

Homologues:

Organism=Homo sapiens, GI11034825, Length=308, Percent_Identity=30.1948051948052, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI33519455, Length=308, Percent_Identity=30.1948051948052, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=40.0684931506849, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLD_RHISN (P55463)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443869.1
- ProteinModelPortal:   P55463
- SMR:   P55463
- GeneID:   962163
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a03570
- HOGENOM:   HBG541114
- ProtClustDB:   CLSK513391
- GO:   GO:0005488
- InterPro:   IPR005913
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01214

Pfam domain/function: PF04321 RmlD_sub_bind

EC number: =1.1.1.133

Molecular weight: Translated: 32236; Mature: 32236

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: BINDING 149-149 BINDING 150-150 BINDING 221-221 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVTGKNGQIALALKAQARPDVEILTLGRPNFDLACRSTVASSIRDAAPDIIVSLAAY
CEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
TAVDKAESEPYEAFAVNRDGVQALAEAAAGLGVPVIHLSTDYVFDGAKPVPYCEEDRTGP
HHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCCC
ISVYGRSKLEGEFAVASANPNHTILRTSWVYSRYGQNFVKKMLRLADTNDELNVVADQLG
EEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
CPTSADDISVAVMTIARRMLSSSSADLRGIFHLSGSGEASWAAFAKYVFSVYDEITGRQI
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
KVHDISAAEYPTPARRPANSRLHCDKLERTFGIRLPNWEESTRRLVWALLLEGKDA
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCC
>Mature Secondary Structure
MRLAVTGKNGQIALALKAQARPDVEILTLGRPNFDLACRSTVASSIRDAAPDIIVSLAAY
CEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
TAVDKAESEPYEAFAVNRDGVQALAEAAAGLGVPVIHLSTDYVFDGAKPVPYCEEDRTGP
HHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCCC
ISVYGRSKLEGEFAVASANPNHTILRTSWVYSRYGQNFVKKMLRLADTNDELNVVADQLG
EEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
CPTSADDISVAVMTIARRMLSSSSADLRGIFHLSGSGEASWAAFAKYVFSVYDEITGRQI
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
KVHDISAAEYPTPARRPANSRLHCDKLERTFGIRLPNWEESTRRLVWALLLEGKDA
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424