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The map label for this gene is rmlA

Identifier: 16519750

GI number: 16519750

Start: 457560

End: 458420

Strand: Reverse

Name: rmlA

Synonym: NGR_a03560

Alternate gene names: 16519750

Gene position: 458420-457560 (Counterclockwise)

Preceding gene: 16519749

Following gene: 16519751

Centisome position: 85.5

GC content: 54.94

Gene sequence:

>861_bases
GTGAAGGGAATTATTCTAGCAGGTGGCAGCGGTACGCGCCTTCACCCGATGACGCTTGTCATGTCAAAGCAAATCTTACC
TGTCTACGACAAGCCGATGATCTTTTATCCGCTGACCACGTTAATGTTGGCCGGAATCCGCGAAATCCTCATCATCTCCA
CGCCGCACCACATGCCTCTCTTCCAGGCATTGCTGGGGGACGGTTCGCAATGGGGGATCTCCTTAAGATATGCTGTTCAG
CCCAGCCCGAACGGGCTGGCGCAGGCCTATGTAATCGGTGCCGATTTCGTAGCTGGTTCACCCTCTTGCCTGATCCTCGG
CGACAACATTTATTTCGGCCACGGCTTGCAGGGACTTTTGCAGCAAGCGGCCGCCCTCCAGCAAGGAGCCACAATTTTTG
CCTATCATGTCAACGATCCGGAGCGCTATGGCGTCGTTGAGTTCGATGAGGGCATGAACGCGCTCTCGATCGAGGAGAAG
CCTGCGGCACCGAAATCGACCTGGGCCGTCACGGGACTTTATTTCTACGATTCCGAAGTTGTTGACATCGCCGCCAATCT
CAAACCTTCAGCGCGTGGCGAATATGAAATCACCGACGTCAACAGAATTTATCTAGAGCGTGGGAAATTGAAGGTTGCTG
TTTTGGGGCGCGGGTACGCTTGGCTCGATACTGGTACTCCCGACAGTTTGCTCGAGGCTGCAGAGTTTGTGCGCACACTC
GAGAAGCGGCAAGCATTCAAAGTTGCCTGCCCGGAGGAAGTCGCTCTGGCAATGGGCTTCATCTCAGTAGAAGAATTTGC
GCGAATTGCGGAGAGGGCCGGAAAGGGAGATTACGGCGCCTATCTTCGGCGGCTTGCGTGA

Upstream 100 bases:

>100_bases
ACTGCGACAAGCTTGAGCGAACCTTTGGTATCCGTCTGCCAAATTGGGAGGAGTCCACGCGCCGGCTGGTTTGGGCGCTG
CTTTTAGAAGGGAAAGATGC

Downstream 100 bases:

>100_bases
AGCGCCGCGTGATCAAGATGGCAACAAGGGCTAGCGACATGCATCTTCTGAACGTTCCGCTCAGTCAGCCGCACTTCTTT
GACCCCCACAGTGCGTGGCT

Product: glucose-1-phopshate thymidinyl transferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKGIILAGGSGTRLHPMTLVMSKQILPVYDKPMIFYPLTTLMLAGIREILIISTPHHMPLFQALLGDGSQWGISLRYAVQ
PSPNGLAQAYVIGADFVAGSPSCLILGDNIYFGHGLQGLLQQAAALQQGATIFAYHVNDPERYGVVEFDEGMNALSIEEK
PAAPKSTWAVTGLYFYDSEVVDIAANLKPSARGEYEITDVNRIYLERGKLKVAVLGRGYAWLDTGTPDSLLEAAEFVRTL
EKRQAFKVACPEEVALAMGFISVEEFARIAERAGKGDYGAYLRRLA

Sequences:

>Translated_286_residues
MKGIILAGGSGTRLHPMTLVMSKQILPVYDKPMIFYPLTTLMLAGIREILIISTPHHMPLFQALLGDGSQWGISLRYAVQ
PSPNGLAQAYVIGADFVAGSPSCLILGDNIYFGHGLQGLLQQAAALQQGATIFAYHVNDPERYGVVEFDEGMNALSIEEK
PAAPKSTWAVTGLYFYDSEVVDIAANLKPSARGEYEITDVNRIYLERGKLKVAVLGRGYAWLDTGTPDSLLEAAEFVRTL
EKRQAFKVACPEEVALAMGFISVEEFARIAERAGKGDYGAYLRRLA
>Mature_286_residues
MKGIILAGGSGTRLHPMTLVMSKQILPVYDKPMIFYPLTTLMLAGIREILIISTPHHMPLFQALLGDGSQWGISLRYAVQ
PSPNGLAQAYVIGADFVAGSPSCLILGDNIYFGHGLQGLLQQAAALQQGATIFAYHVNDPERYGVVEFDEGMNALSIEEK
PAAPKSTWAVTGLYFYDSEVVDIAANLKPSARGEYEITDVNRIYLERGKLKVAVLGRGYAWLDTGTPDSLLEAAEFVRTL
EKRQAFKVACPEEVALAMGFISVEEFARIAERAGKGDYGAYLRRLA

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family

Homologues:

Organism=Homo sapiens, GI11761621, Length=283, Percent_Identity=25.4416961130742, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11761619, Length=283, Percent_Identity=25.4416961130742, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788351, Length=285, Percent_Identity=64.9122807017544, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=63.5087719298246, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI133931050, Length=239, Percent_Identity=25.5230125523013, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=280, Percent_Identity=26.7857142857143, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355443, Length=275, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644084, Length=275, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLA_RHISN (P55464)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443870.1
- ProteinModelPortal:   P55464
- SMR:   P55464
- GeneID:   962578
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a03560
- HOGENOM:   HBG688195
- ProtClustDB:   CLSK2518392
- InterPro:   IPR005907
- InterPro:   IPR005835
- TIGRFAMs:   TIGR01207

Pfam domain/function: PF00483 NTP_transferase

EC number: =2.7.7.24

Molecular weight: Translated: 31205; Mature: 31205

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLHPMTLVMSKQILPVYDKPMIFYPLTTLMLAGIREILIISTPHHMPL
CCCEEEECCCCCEECCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHH
FQALLGDGSQWGISLRYAVQPSPNGLAQAYVIGADFVAGSPSCLILGDNIYFGHGLQGLL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECHHCCCCCCEEEEECCEEECCCHHHHH
QQAAALQQGATIFAYHVNDPERYGVVEFDEGMNALSIEEKPAAPKSTWAVTGLYFYDSEV
HHHHHHHCCCEEEEEECCCCHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCE
VDIAANLKPSARGEYEITDVNRIYLERGKLKVAVLGRGYAWLDTGTPDSLLEAAEFVRTL
EEEEECCCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHH
EKRQAFKVACPEEVALAMGFISVEEFARIAERAGKGDYGAYLRRLA
HHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLHPMTLVMSKQILPVYDKPMIFYPLTTLMLAGIREILIISTPHHMPL
CCCEEEECCCCCEECCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHH
FQALLGDGSQWGISLRYAVQPSPNGLAQAYVIGADFVAGSPSCLILGDNIYFGHGLQGLL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECHHCCCCCCEEEEECCEEECCCHHHHH
QQAAALQQGATIFAYHVNDPERYGVVEFDEGMNALSIEEKPAAPKSTWAVTGLYFYDSEV
HHHHHHHCCCEEEEEECCCCHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCE
VDIAANLKPSARGEYEITDVNRIYLERGKLKVAVLGRGYAWLDTGTPDSLLEAAEFVRTL
EEEEECCCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHH
EKRQAFKVACPEEVALAMGFISVEEFARIAERAGKGDYGAYLRRLA
HHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424