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The map label for this gene is rmlB

Identifier: 16519748

GI number: 16519748

Start: 459311

End: 460363

Strand: Reverse

Name: rmlB

Synonym: NGR_a03580

Alternate gene names: 16519748

Gene position: 460363-459311 (Counterclockwise)

Preceding gene: 16519747

Following gene: 16519749

Centisome position: 85.86

GC content: 54.99

Gene sequence:

>1053_bases
ATGCGCATTTTGGTGACTGGTGGCGCCGGCTTTATCGGATCAGCGCTGGTTCGGTATCTCGTGAGCATCAACGCGGAGGT
CCTGAACGTTGACAAGTTGACCTACGCTGGCAACCTCGCTTCGCTGAAGCCGGTCGAAGGTCTCCGCAACTATCGGTTCC
TTCGCGCCGATATCTGCGACCGAGTGGCGATAAACGAAGCTTTCGAGACGTTTCAGCCGGATTACGTCATTCATCTGGCG
GCGGAAAGTCACGTAGATCGCTCGATCACCGGAGCGGACGACTTCGTCCAGACTAACGTGAACGGAACTTTCACAATGCT
GGAGACAGCGCGGCAATACTGGAGCAATCTGTCCCAGAATCGGAAGGCATTCTTTAAGATGCTGCATGTGTCGACCGACG
AGGTTTATGGCTCACTTGGAGACCGCGGTCAGTTCGAGGAGGTTTCACCGTACGACCCATCTTCTCCCTACTCGGCTTCA
AAGGCGGCGAGCGACCATTTTGCAACCGCATGGCAGCGAACATATGGGCTTCCCGTGGTCATTTCGAATTGCTCCAACAA
CTATGGACCGTTCCACTTCCCCGAGAAACTGATCCCGCTGATGATTCTCAATGCATTGGATAGGAAGCCTTTGCCCGTCT
ATGGGACGGGTTCCAACATTCGCGATTGGCTCTATGTCGACGACCATGCCCGAGCCCTTTGGCTGATCGTCAGGGAAGGC
CGTCCTGGTGAGAAATACAATGTCGGAGGTCGCAACGAGTTGCGCAATATCGACGTCGTCAACCGCATATGCTTGCTCCT
CGATGAGCTTAGTCCCAACGCTTCGCACTATGGTGACCTAATTACTTTCGTGAAAGACAGGCCGGGTCACGACGCACGCT
ACGCCATTGACGCCACGAAGCTCGAAACCGAGCTTGGCTGGAAGGCGCAGGAGAATTTCGATACCGGCATACGCAAAACG
GTGGAATGGTATCTGGAAAATGGCTGGTGGTGGCAACCGCTGCGGGACAAGGTTTATTCCGGTGAGCGCCTCGGTCTCCT
GGAGAAAGCGTGA

Upstream 100 bases:

>100_bases
CTGCGGGGTGGCTCCTGAATCTCAATAACAGCGCGCCGCCCCGAACGAACGCGCTATCCGCGGCGAGGAGTCTGCACCTG
CGCACCACAGCGAGAAGTTC

Downstream 100 bases:

>100_bases
CAGATGCGGCTCGCGGTGACCGGCAAAAACGGACAAATCGCCCTCGCTTTGAAGGCGCAGGCGCGACCTGACGTCGAGAT
ACTTACTTTGGGGCGGCCGA

Product: dTDP-glucose 4,6-dehydratase RmlB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRILVTGGAGFIGSALVRYLVSINAEVLNVDKLTYAGNLASLKPVEGLRNYRFLRADICDRVAINEAFETFQPDYVIHLA
AESHVDRSITGADDFVQTNVNGTFTMLETARQYWSNLSQNRKAFFKMLHVSTDEVYGSLGDRGQFEEVSPYDPSSPYSAS
KAASDHFATAWQRTYGLPVVISNCSNNYGPFHFPEKLIPLMILNALDRKPLPVYGTGSNIRDWLYVDDHARALWLIVREG
RPGEKYNVGGRNELRNIDVVNRICLLLDELSPNASHYGDLITFVKDRPGHDARYAIDATKLETELGWKAQENFDTGIRKT
VEWYLENGWWWQPLRDKVYSGERLGLLEKA

Sequences:

>Translated_350_residues
MRILVTGGAGFIGSALVRYLVSINAEVLNVDKLTYAGNLASLKPVEGLRNYRFLRADICDRVAINEAFETFQPDYVIHLA
AESHVDRSITGADDFVQTNVNGTFTMLETARQYWSNLSQNRKAFFKMLHVSTDEVYGSLGDRGQFEEVSPYDPSSPYSAS
KAASDHFATAWQRTYGLPVVISNCSNNYGPFHFPEKLIPLMILNALDRKPLPVYGTGSNIRDWLYVDDHARALWLIVREG
RPGEKYNVGGRNELRNIDVVNRICLLLDELSPNASHYGDLITFVKDRPGHDARYAIDATKLETELGWKAQENFDTGIRKT
VEWYLENGWWWQPLRDKVYSGERLGLLEKA
>Mature_350_residues
MRILVTGGAGFIGSALVRYLVSINAEVLNVDKLTYAGNLASLKPVEGLRNYRFLRADICDRVAINEAFETFQPDYVIHLA
AESHVDRSITGADDFVQTNVNGTFTMLETARQYWSNLSQNRKAFFKMLHVSTDEVYGSLGDRGQFEEVSPYDPSSPYSAS
KAASDHFATAWQRTYGLPVVISNCSNNYGPFHFPEKLIPLMILNALDRKPLPVYGTGSNIRDWLYVDDHARALWLIVREG
RPGEKYNVGGRNELRNIDVVNRICLLLDELSPNASHYGDLITFVKDRPGHDARYAIDATKLETELGWKAQENFDTGIRKT
VEWYLENGWWWQPLRDKVYSGERLGLLEKA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=37.2372372372372, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI42516563, Length=329, Percent_Identity=26.4437689969605, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=61.5384615384615, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=59.025787965616, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1786974, Length=364, Percent_Identity=26.6483516483516, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=339, Percent_Identity=23.598820058997, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788365, Length=343, Percent_Identity=23.3236151603499, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=34.4213649851632, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=33.5365853658537, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=24.3161094224924, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=250, Percent_Identity=23.2, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=250, Percent_Identity=23.2, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=250, Percent_Identity=23.2, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=250, Percent_Identity=23.2, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=250, Percent_Identity=23.2, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=250, Percent_Identity=23.2, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=258, Percent_Identity=28.6821705426357, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=25.531914893617, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=27.247191011236, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBB_RHISN (P55462)

Other databases:

- EMBL:   U00090
- RefSeq:   NP_443868.1
- ProteinModelPortal:   P55462
- SMR:   P55462
- GeneID:   962310
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a03580
- HOGENOM:   HBG755066
- ProtClustDB:   CLSK893853
- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01181

Pfam domain/function: PF01370 Epimerase

EC number: =4.2.1.46

Molecular weight: Translated: 39665; Mature: 39665

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: ACT_SITE 133-133 ACT_SITE 134-134 ACT_SITE 157-157 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGAGFIGSALVRYLVSINAEVLNVDKLTYAGNLASLKPVEGLRNYRFLRADICD
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHEEECCCCCCCCHHHHHHHHHHHHHHHHH
RVAINEAFETFQPDYVIHLAAESHVDRSITGADDFVQTNVNGTFTMLETARQYWSNLSQN
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHH
RKAFFKMLHVSTDEVYGSLGDRGQFEEVSPYDPSSPYSASKAASDHFATAWQRTYGLPVV
HHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEE
ISNCSNNYGPFHFPEKLIPLMILNALDRKPLPVYGTGSNIRDWLYVDDHARALWLIVREG
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEEEEEECC
RPGEKYNVGGRNELRNIDVVNRICLLLDELSPNASHYGDLITFVKDRPGHDARYAIDATK
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCEEEEEHHH
LETELGWKAQENFDTGIRKTVEWYLENGWWWQPLRDKVYSGERLGLLEKA
HHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRILVTGGAGFIGSALVRYLVSINAEVLNVDKLTYAGNLASLKPVEGLRNYRFLRADICD
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHEEECCCCCCCCHHHHHHHHHHHHHHHHH
RVAINEAFETFQPDYVIHLAAESHVDRSITGADDFVQTNVNGTFTMLETARQYWSNLSQN
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHH
RKAFFKMLHVSTDEVYGSLGDRGQFEEVSPYDPSSPYSASKAASDHFATAWQRTYGLPVV
HHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCEE
ISNCSNNYGPFHFPEKLIPLMILNALDRKPLPVYGTGSNIRDWLYVDDHARALWLIVREG
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEEEEEECC
RPGEKYNVGGRNELRNIDVVNRICLLLDELSPNASHYGDLITFVKDRPGHDARYAIDATK
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCEEEEEHHH
LETELGWKAQENFDTGIRKTVEWYLENGWWWQPLRDKVYSGERLGLLEKA
HHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424