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The map label for this gene is sppA [C]

Identifier: 15668832

GI number: 15668832

Start: 579515

End: 580450

Strand: Reverse

Name: sppA [C]

Synonym: MJ0651

Alternate gene names: 15668832

Gene position: 580450-579515 (Counterclockwise)

Preceding gene: 15668833

Following gene: 15668829

Centisome position: 34.86

GC content: 31.73

Gene sequence:

>936_bases
ATGAAAAAAATTTATATCATTCTTCTAATTCTTTTTGTGATTTTAATTAGTTTAATAGGGGCAAGTATATTATTAGTTAT
GAGCTTATCAGGAGAAAATGTCGATTTGTTTGGTGGGGAAAAAATAGCCAAGGTTTATTTATGCAATGAAATCTATTTTG
ATTATAATCAAGGAGATGGAATCTTTCCACAACAAAAAAAAGATGCAAGATATTATATAAATTTATTAGATGATTTAGAG
AAGGACGATTCAGTTAAAGGGGTTTTATTGGTTGTTAATTCTCCCGGAGGAGAGGTTATAGCAAGTGAGAAATTAGCAAG
AAAGGTTGAAGAACTTGCAAAGAAAAAGCCAGTAGTTGTTTATGTTGAGGGCTTAGATGCTTCTGGTGCTTATATGGTTT
CAGCCCCTGCAGATTATATAGTGGCTGAAAAGCACTCAATAGTAGGAAGTATTGGGGTTAGGATGGATTTAATGCACTAT
TATGGATTGATGAAAAAGCTTGGTATAAATGTAACTACAATAAAAGCTGGAAAGTATAAGGATATTGGTTCTCCATTTAG
ACCAATGACTAAAGAAGAAAAGGAATACCTACAAAAAATGATAAATGAAACATACATGGATTTTGTTAAATGGGTAGCAG
AGCATAGGCATTTGTCAATAAACTACACTTTAAAAATAGCAGATGGAAAGATATATAGTGGGGAGGATGCTAAAAAAGTT
GGATTAGTTGATGAAGTGGGAACTGAAGAAGATGCTTTGAAAAAATTAGAACAGTTAGCTAATGTCTCAAATCCTGAGAT
TGTTGAATATGGCTTAGAAGAGAATAAAGGATTGTTTGGATTAACATACTATTTAGGTTATGGAATTGGAAAAGGAATTG
GAGAAGTTTTATATGGAATGGAAAAGATTAATGGAAGAGTTGAGTTATTAAGTTAA

Upstream 100 bases:

>100_bases
AAAAAACCAAAGAAAAAGGTTAAAAAGGATGAAAAGATTATTGAATTATTTGAGAAGTTTAAGAAGAAGGCAGTTAAAGA
ATAAATAATTGAGGGAGATA

Downstream 100 bases:

>100_bases
TTTCTTATTAATTTTTACCACTATATAAATGGCTATAATTGTTAGTATAATAAATATGAGAATATATAAGTAATTATTAT
ACGAGGATTTCTCTTTATAG

Product: protease IV SppA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDGIFPQQKKDARYYINLLDDLE
KDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVVYVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHY
YGLMKKLGINVTTIKAGKYKDIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKV
GLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGMEKINGRVELLS

Sequences:

>Translated_311_residues
MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDGIFPQQKKDARYYINLLDDLE
KDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVVYVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHY
YGLMKKLGINVTTIKAGKYKDIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKV
GLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGMEKINGRVELLS
>Mature_311_residues
MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDGIFPQQKKDARYYINLLDDLE
KDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVVYVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHY
YGLMKKLGINVTTIKAGKYKDIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKV
GLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGMEKINGRVELLS

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family

Homologues:

Organism=Escherichia coli, GI1788064, Length=198, Percent_Identity=35.8585858585859, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787527, Length=179, Percent_Identity=32.9608938547486, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y651_METJA (Q58067)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247635.1
- ProteinModelPortal:   Q58067
- SMR:   Q58067
- MEROPS:   S49.001
- GeneID:   1451517
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0651
- NMPDR:   fig|243232.1.peg.672
- TIGR:   MJ0651
- HOGENOM:   HBG294245
- OMA:   MINETYM
- ProtClustDB:   CLSK876279
- BioCyc:   MJAN243232:MJ_0651-MONOMER
- GO:   GO:0006508
- InterPro:   IPR004635
- InterPro:   IPR002142
- TIGRFAMs:   TIGR00706

Pfam domain/function: PF01343 Peptidase_S49

EC number: 3.4.21.-

Molecular weight: Translated: 34666; Mature: 34666

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDG
CCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEEEEEEEEEEEEECCCCC
IFPQQKKDARYYINLLDDLEKDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVV
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCEEE
YVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHYYGLMKKLGINVTTIKAGKYK
EEECCCCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
DIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCEEECCCCCHHC
GLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGM
CCHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
EKINGRVELLS
HHCCCEEEEEC
>Mature Secondary Structure
MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDG
CCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEEEEEEEEEEEEECCCCC
IFPQQKKDARYYINLLDDLEKDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVV
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCEEE
YVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHYYGLMKKLGINVTTIKAGKYK
EEECCCCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
DIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCEEECCCCCHHC
GLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGM
CCHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
EKINGRVELLS
HHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087