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The map label for this gene is lpdA [C]

Identifier: 15668817

GI number: 15668817

Start: 565878

End: 567071

Strand: Direct

Name: lpdA [C]

Synonym: MJ0636

Alternate gene names: 15668817

Gene position: 565878-567071 (Clockwise)

Preceding gene: 15668816

Following gene: 15668823

Centisome position: 33.99

GC content: 30.82

Gene sequence:

>1194_bases
TTGTTATTTGGTGAAAGTATGACATTAAAGATAGCTGTTGTTGGAGCAGGTCCTGCAGGAAGAACATCGGCTATGTTTTT
GGCAAAAAATGGCTTTGATGTTGATTTATTTGAAAAAGATAGAGTTGGAGGAACTTGTTTAAACTATGGATGCACATATA
TAACTGGATTAAGAGAAATGGCAGATATTATTAATAATTTAAGTATTTTAAAAGGAGAGAAGGTTCATTTAGAGGAAATA
ATTTCATTTAAAGAGTTGCAGGAAAAAATAAATAAAATTCAGGATAGAATTAGGAATAAATTAGAAAAAGAAACAAAAGA
ACTTGGAGTAAATATAAAATACAAAGAGTTTAAGAATAAGCATAAGAACGATTATGATTACATCATCTATGCAACTGGAA
GAAATTATCCTTCCAATTATAATGGATATGAGGTTTTAACTCACAAAGATATACCAAATTTAAGAGAACTGCCAGAAAAT
ATCTTAATTATTGGTGGAGGAGTTGTAGCTACCGAATATGCTTCAATATTTTCTGACTTTGGCTGTAATGTTGTTCTATA
CACAAGGTCTAAGATTTTGAAAGAGATTAAGGATGAGGAAATTAGAGATTACTTAATGAAGAAAGTTATAAACTTTAAAA
TAATCAATGACAAAGAAGAGCTTGAAAATCTGTTAAAGGATGAAAGTTATACAAAAATCTTAGCCATTGGTGGAAATGGC
AGATTTAAGACAGATGATTACTTGAGGGTTTTAAATGAAGAGAAAGTTTATGCATGTGGAGATTGTTTAATCAACGGTGG
AGGAAATACTCCAATATCAAGGATGGAGGGGAGAGTGGTAGCCCAAAATATCTACAATGAAATAAACAATAAGCCTCTAA
TAAAACCAAATTATGAATTAATTCCAAAGACTATAAGGCTGTCTTTAACTATATCTTATGTAGGGAAGCAGACAAATAAT
TATAAAACAATAAGAAGCTGTGTAGGGAAGGGGAATTTCTTTAAAGTTTTGAGTGGTGTTGGGATAAATAAAATCTATTA
TGAAGATGGAAAAATTGTTGGAGCTATAACCATGATGCCTTGTGCTGAAATTCTTCCATACTTCACTCAGTTAATTAGGG
GGATAGATGTTTATAACAACTTCATGGAAGTTCATCCATCAACTGACATATTTTATAAAGAGTTCAGGTCATAA

Upstream 100 bases:

>100_bases
TATTGTTGTTGTTCTTATTTTTTATTTGATTAAAAAATTTAATTGTAATTGTTATTAACCATTAACTTTATATTCAATAA
CATTAAACCAATTTTTAATT

Downstream 100 bases:

>100_bases
AAACAAAGAACAAAAAAATAATTAATCAAAAACTGGGTATTTTCCTAAAAGATTGATAAATGTTGTATGCCTCTCCAAAG
ATTTTAAAATTTCTTCTAAC

Product: dihydrolipoamide dehydrogenase

Products: protein N6-(lipoyl)lysine; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREMADIINNLSILKGEKVHLEEI
ISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNKHKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPEN
ILIIGGGVVATEYASIFSDFGCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNG
RFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYELIPKTIRLSLTISYVGKQTNN
YKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMPCAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS

Sequences:

>Translated_397_residues
MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREMADIINNLSILKGEKVHLEEI
ISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNKHKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPEN
ILIIGGGVVATEYASIFSDFGCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNG
RFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYELIPKTIRLSLTISYVGKQTNN
YKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMPCAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS
>Mature_397_residues
MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREMADIINNLSILKGEKVHLEEI
ISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNKHKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPEN
ILIIGGGVVATEYASIFSDFGCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNG
RFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYELIPKTIRLSLTISYVGKQTNN
YKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMPCAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): Y636_METJA (Q58053)

Other databases:

- EMBL:   L77117
- PIR:   D64379
- RefSeq:   NP_247620.1
- ProteinModelPortal:   Q58053
- GeneID:   1451502
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0636
- NMPDR:   fig|243232.1.peg.657
- TIGR:   MJ0636
- HOGENOM:   HBG539714
- OMA:   AEYASIF
- ProtClustDB:   CLSK876274
- BioCyc:   MJAN243232:MJ_0636-MONOMER
- InterPro:   IPR013027
- InterPro:   IPR001327

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: 1.8.1.4

Molecular weight: Translated: 45233; Mature: 45233

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREM
CCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHCCHHHHHHHHHH
ADIINNLSILKGEKVHLEEIISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNK
HHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCC
HKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPENILIIGGGVVATEYASIFSDF
CCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHCCCCEEEEECCHHHHHHHHHHHHC
GCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNG
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHCCCCCEEEEEECCCC
RFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYEL
CCCCHHHHHHHCCCCEEEECCEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
IPKTIRLSLTISYVGKQTNNYKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMP
CCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCEEEEECCEEEEEEECCC
CAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREM
CCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCHHHHCCHHHHHHHHHH
ADIINNLSILKGEKVHLEEIISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNK
HHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCC
HKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPENILIIGGGVVATEYASIFSDF
CCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHCCCCEEEEECCHHHHHHHHHHHHC
GCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNG
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHCCCCCEEEEEECCCC
RFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYEL
CCCCHHHHHHHCCCCEEEECCEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
IPKTIRLSLTISYVGKQTNNYKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMP
CCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCEEEEECCEEEEEEECCC
CAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: protein N6-(dihydrolipoyl)lysine; NAD+

Specific reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087