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The map label for this gene is nth [C]

Identifier: 15668794

GI number: 15668794

Start: 543933

End: 544967

Strand: Reverse

Name: nth [C]

Synonym: MJ0613

Alternate gene names: 15668794

Gene position: 544967-543933 (Counterclockwise)

Preceding gene: 15668795

Following gene: 15668790

Centisome position: 32.73

GC content: 27.34

Gene sequence:

>1035_bases
ATGGAGTTAATTGAAATATTGCTAAAAAAACTAAACAAAAATGCAGTAGTTACAGAGATAGCCAAAGATAAAGACCCTTT
TAAGGTTTTAATATCAACTATAATAAGTGCAAGAACAAAGGATGAAGTAACTGAAGAGGTTTCTAAAAAACTATTTAAAG
AGATTAAGGATGTTGATGATTTATTAAACATAGATGAAGAAAAATTAGCAGATTTGATATACCCAGCAGGATTTTATAAA
AATAAGGCAAAAAATTTAAAAAAATTAGCCAAAATTTTAAAAGAAAATTATAATGGGAAAGTTCCAGATTCTTTGGAAGA
GTTGTTAAAGCTCCCAGGGGTTGGAAGGAAAACAGCTAATTTGGTTATAACCTTAGCTTTCAACAAAGATGGGATTTGTG
TAGATACCCATGTCCATAGGATATGTAATAGATGGGAAATAGTTGATACTGAGACTCCTGAAGAGACAGAGTTTGAATTA
AGAAAAAAGCTTCCTAAAAAATATTGGAAAGTAATAAATAATTTGTTGGTGGTTTTTGGAAGGGAGATTTGTTCTTCAAA
ATCTAAGTGTGATAAATGTTTTAAAGAAATTAAAGAGAAATGCCCTTACTATGAAAAAATTAAGCACTTTGAAAATATAT
TAAAAAAATTCAATTTTAGAAAAGTCTCAAAAAACAAAATCCCTAATGAAAAAGGAACTTACATCTTAAAAATTAGGTTA
AAAGAAGGTAAAAAAATAAAATTTGGAAAAACAGAGAGATTTTTTAAAAAAGGATATTATTTCTACATTGGCTCTGCCTT
TGGAAATTCAATGAACTTAAAAAATAGGATAGAGAGGCATTTAAAGGATGATAAAAAGATGCACTGGCATATTGATTATT
TATTAAAATATGGTAAGATTGAAGAGATTTATATTACAAATGAGAGAGTTGAGTGTGAGGTTGCAAATGAATTTATAAAA
AAATTTGATTTTGTTGAGAACTTTGGATGTTCTGATTGTAAATGTAAGAGTCATTTATTTTATTTGAAACCATAG

Upstream 100 bases:

>100_bases
AAAGAGAGTAAGTAATTTACTAAAAACTTTTTTATTATTTCTTTATAGAAAATTTAACAAAATTTTATTATTTTTGAAAG
ATGCTAATTTTGGGATTCCT

Downstream 100 bases:

>100_bases
AGGGGGCGTAGCCCCCTCTATGGTGTGGATACCCAGAGCGGGGCTTCACTACGTTCAGCCCCACTTAATTAAGAGGCATT
GCCGAGTGAAGCGAGGTAAT

Product: endonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDDLLNIDEEKLADLIYPAGFYK
NKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTANLVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFEL
RKKLPKKYWKVINNLLVVFGREICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRL
KEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKIEEIYITNERVECEVANEFIK
KFDFVENFGCSDCKCKSHLFYLKP

Sequences:

>Translated_344_residues
MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDDLLNIDEEKLADLIYPAGFYK
NKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTANLVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFEL
RKKLPKKYWKVINNLLVVFGREICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRL
KEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKIEEIYITNERVECEVANEFIK
KFDFVENFGCSDCKCKSHLFYLKP
>Mature_344_residues
MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDDLLNIDEEKLADLIYPAGFYK
NKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTANLVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFEL
RKKLPKKYWKVINNLLVVFGREICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRL
KEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKIEEIYITNERVECEVANEFIK
KFDFVENFGCSDCKCKSHLFYLKP

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=185, Percent_Identity=40.5405405405405, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=34.1463414634146, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789331, Length=227, Percent_Identity=25.9911894273128, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=170, Percent_Identity=44.7058823529412, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=38.7640449438202, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324530, Length=207, Percent_Identity=36.7149758454106, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=38.8888888888889, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y613_METJA (Q58030)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247597.1
- ProteinModelPortal:   Q58030
- SMR:   Q58030
- GeneID:   1451479
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0613
- NMPDR:   fig|243232.1.peg.634
- TIGR:   MJ0613
- HOGENOM:   HBG541186
- OMA:   CKCKSHL
- ProtClustDB:   CLSK876263
- BioCyc:   MJAN243232:MJ_0613-MONOMER
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR002837
- InterPro:   IPR004036
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR000305
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00525
- SMART:   SM00465
- SMART:   SM00278

Pfam domain/function: PF01986 DUF123; PF00633 HHH; PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: 4.2.99.18

Molecular weight: Translated: 40568; Mature: 40568

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDD
CHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNIDEEKLADLIYPAGFYKNKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTAN
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFELRKKLPKKYWKVINNLLVVFG
EEEEEEECCCCEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRL
HHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEE
KEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKI
CCCCEECCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHEEHHHHHHHCCCE
EEIYITNERVECEVANEFIKKFDFVENFGCSDCKCKSHLFYLKP
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDD
CHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNIDEEKLADLIYPAGFYKNKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTAN
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFELRKKLPKKYWKVINNLLVVFG
EEEEEEECCCCEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRL
HHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEE
KEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKI
CCCCEECCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHEEHHHHHHHCCCE
EEIYITNERVECEVANEFIKKFDFVENFGCSDCKCKSHLFYLKP
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087