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The map label for this gene is mjaIIIR

Identifier: 15668780

GI number: 15668780

Start: 531176

End: 532048

Strand: Direct

Name: mjaIIIR

Synonym: MJ0600

Alternate gene names: 15668780

Gene position: 531176-532048 (Clockwise)

Preceding gene: 15668779

Following gene: 15668781

Centisome position: 31.9

GC content: 23.94

Gene sequence:

>873_bases
ATGAATTTTGAATACATCATTAACAGCCTCTTAGAAACCATAAAAACCTACAATTTCTTTGTAGATTGGGAAAAAATTGA
AAATAATATAAAAAAGATAGAGAAAAGATTACATATCCTAAATTATCTAATTGGAAAAGAGAATTTTAAAGAGGAGTTTT
TTGAATTACTAAAAGAGTATCCAGAGGTTATTACTGTTTTTCCAATTTTAATAGCTGTTAGAGATAACAAAATAACAATT
TTAAACGAAAATATGGAATTAGAAACCTTAGAATTTAAAGAAAAGAAATATTTAACTGATGAAGAAATTGAAAGATATTA
TAAATTTTTTAAAGAGACTGGATTGGAGGATTTGTTAAAAAATAGAAAAATAAAGAATTTAGTGGATTATGTTTTTGGTG
TTGAGGTAGGAATGGACACCAATGCAAGGAAAAATAGAATTGGAGATTTAATGGAAAATATTGTTAAAAAATATATTGAA
AATCTATGCAAACAAAATAAAAACCTTGATTATATCTTCCAAGCAACAAAAGATAAAATAAAACAAAAATGGGGAATAAA
TTTAACATTGGATAAAACAAATAGAAAATTTGATTTTGCAGTTTTTAATAAAAACACTAAAAAGTTATATCTCATTGAAG
TTAATTTTTATAGTGGAGGAGGTTCAAAGTTAAAAGCTACTGCTGGAGAGTATAGATCATTAAATGAATTTATAAAAAAC
AATAACAATAATGTTCAATTTATATGGATAACTGATGGAAAGGGATGGAATACAGCAAAGAATCCATTAAAAGAAAGTTT
TAATAGTGGAGTTGTTATATTAAACTTAAAAATGGTTAAAGAAGGATTATTAAAAGAAATATTAACTCAGTAA

Upstream 100 bases:

>100_bases
ATGAAAAATGTGATGAGATTTATAAAATGGCAAAAAAGAAATTGTAAAGAATTGTATAACGAATTAACGAAGAAACCATC
AAAATAATTAAGGGATATTT

Downstream 100 bases:

>100_bases
TATTAGATTTAAAATTGTGAATGATACCATTATTCAATTTCCAAAATGTATAAAAACTTGATCTTGAAAATAATATTGAA
ACTAAACTTTAAATAATATT

Product: type II R/M system restriction enzyme 2

Products: NA

Alternate protein names: R.MjaIII; Endonuclease MjaIII; Type II restriction enzyme MjaIII

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEYPEVITVFPILIAVRDNKITI
LNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLKNRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIE
NLCKQNKNLDYIFQATKDKIKQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKN
NNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ

Sequences:

>Translated_290_residues
MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEYPEVITVFPILIAVRDNKITI
LNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLKNRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIE
NLCKQNKNLDYIFQATKDKIKQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKN
NNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ
>Mature_290_residues
MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEYPEVITVFPILIAVRDNKITI
LNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLKNRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIE
NLCKQNKNLDYIFQATKDKIKQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKN
NNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ

Specific function: Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme DpnII family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): T2M3_METJA (Q58017)

Other databases:

- EMBL:   L77117
- PIR:   H64374
- RefSeq:   NP_247582.1
- ProteinModelPortal:   Q58017
- REBASE:   3891
- GeneID:   1451465
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0600
- NMPDR:   fig|243232.1.peg.619
- TIGR:   MJ0600
- HOGENOM:   HBG288087
- OMA:   TNARKNR
- ProtClustDB:   CLSK2747636
- BioCyc:   MJAN243232:MJ_0600-MONOMER
- BRENDA:   3.1.21.4
- InterPro:   IPR021191
- InterPro:   IPR007637
- PIRSF:   PIRSF016080

Pfam domain/function: PF04556 DpmII

EC number: =3.1.21.4

Molecular weight: Translated: 34392; Mature: 34392

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEY
CCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PEVITVFPILIAVRDNKITILNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLK
HHHHHHHHHHHEEECCEEEEEECCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
NRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIENLCKQNKNLDYIFQATKDKI
CCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKN
HHHHCCEEEEECCCCEEEEEEEECCCCEEEEEEEEEECCCCCEEEECHHHHHHHHHHHHC
NNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEY
CCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PEVITVFPILIAVRDNKITILNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLK
HHHHHHHHHHHEEECCEEEEEECCCCEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
NRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIENLCKQNKNLDYIFQATKDKI
CCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKN
HHHHCCEEEEECCCCEEEEEEEECCCCEEEEEEEEEECCCCCEEEECHHHHHHHHHHHHC
NNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087