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The map label for this gene is hyfG [C]

Identifier: 15668694

GI number: 15668694

Start: 457622

End: 458764

Strand: Reverse

Name: hyfG [C]

Synonym: MJ0515

Alternate gene names: 15668694

Gene position: 458764-457622 (Counterclockwise)

Preceding gene: 15668695

Following gene: 15668693

Centisome position: 27.55

GC content: 36.05

Gene sequence:

>1143_bases
ATGAGTGAAAATATGGCAACAATTCCTATAGGACCAATTCATCCAGTATTGAAAGAGCCGTTAAGGATTAAACTTGTTTT
AGATGGAGAGAAACCTGTTGATGCTGAAATTGAAATGGGTTATGTTCATAGAGGAATAGAAAAAATTATGGAAGGAAAAC
ATTGCCATAAAGGAATTCACTTAGCAGAAAGAGTTTGTGGTATCTGTTCCTATGTGCATACGATGACGTTTGCTGAATGC
ATTGAGCATATATCAAAGATAGAGATTCCAGACAAGGCAAAATATCTTAGGGTAGTTACTTGTGAATTAGAGAGAATACA
CAGCCATTTAATTGCTTCAGCAGTGTATAATTTATCTATTGAACATGAAACACTTGCTATGTGGCTTTTGAATGTTAGGG
AAATAATTATGGATTTAATGGAGATGATTACTGGAAATAGGGTTAATATGGGTTATAATGTAATTGGGGGAGTTAGAAGA
GACATAAATAGAGAGATGATGGATGAGATATATAAAAAACTCGATATCTTTGAAGATGAACTAAAAAATATTATTGAGGT
TTTTGAAACAGGGCCTTTAATAGCTTTAAGAAGTAAAGAAATTGGTATTTTGCCATATCATGAAGTTATGAGGACGAGGG
CTGTTGGGCCAATTTGTAGAGGTTCTGGATTGCCAGAAAGTGATTGGAGGTTAAGACATTCAACATATCAAGAGTTGAAA
TTTAAGCCAGTGTGGAGGGAGGAAGGAGATAACTTTGCAAGGATGATGGTTAGGCATGAAGAGATTATTGAGAGCGTTAG
ATTAATTAGAGAGGCTTTAGAGCATTATGAAGAGTGTTCTGGAGATATAAGGGTTAAGGCAGAGATTAAAGGAGGAAAAG
GAGAGTGGAGGAATGAAGCTCCAAGAGGAGAGGTAACTTATAGGATGGAAATAACTGATGGAGGGATAATAAAGAGGATA
ATGATTAGAACTCCTACAGTTATGAACTTGGAGGCGTATAAATATATGCTAAAGACTTGTCCAACTGTAGCTGATGCTGT
ATCTGCTTATACAAGTATCGACCCTTGCGTTTCATGCACAGAGAGATGCATAGTTGCAGTAAAGGATGGCAAGGAGATTC
CAATTAGTATTAAATTTAGGTGA

Upstream 100 bases:

>100_bases
ATCCCTGGATGTCCTCCAAGACCTTCTGAGATTATTGAAACAATCTTAAAGGTAGCTCCTAAGGCAATAGCAATGAGAGA
AAAGAGATTAAAAAATAAAG

Downstream 100 bases:

>100_bases
TTGTTATGGCATCTTCGCTATGGTATCTTTATGAATTTGCAAGAAAAAAGTGGATTAAAAGATTTATTGATGCAAAATCA
GATAAAAGCTCCTATATTCC

Product: carbon monoxide dehydrogenase hydrogenase subunit CooH

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIHLAERVCGICSYVHTMTFAEC
IEHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSIEHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRR
DINREMMDEIYKKLDIFEDELKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELK
FKPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEAPRGEVTYRMEITDGGIIKRI
MIRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCTERCIVAVKDGKEIPISIKFR

Sequences:

>Translated_380_residues
MSENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIHLAERVCGICSYVHTMTFAEC
IEHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSIEHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRR
DINREMMDEIYKKLDIFEDELKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELK
FKPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEAPRGEVTYRMEITDGGIIKRI
MIRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCTERCIVAVKDGKEIPISIKFR
>Mature_379_residues
SENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIHLAERVCGICSYVHTMTFAECI
EHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSIEHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRRD
INREMMDEIYKKLDIFEDELKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELKF
KPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEAPRGEVTYRMEITDGGIIKRIM
IRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCTERCIVAVKDGKEIPISIKFR

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family

Homologues:

Organism=Homo sapiens, GI4758786, Length=283, Percent_Identity=27.208480565371, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI260898743, Length=283, Percent_Identity=27.208480565371, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788832, Length=371, Percent_Identity=30.7277628032345, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789076, Length=362, Percent_Identity=31.4917127071823, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI145693162, Length=292, Percent_Identity=29.7945205479452, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17555284, Length=358, Percent_Identity=25.6983240223464, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568379, Length=360, Percent_Identity=25, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24638644, Length=273, Percent_Identity=30.03663003663, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221459469, Length=271, Percent_Identity=28.0442804428044, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y515_METJA (Q57935)

Other databases:

- EMBL:   L77117
- PIR:   C64364
- RefSeq:   NP_247493.1
- ProteinModelPortal:   Q57935
- GeneID:   1451379
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0515
- NMPDR:   fig|243232.1.peg.530
- TIGR:   MJ0515
- HOGENOM:   HBG459705
- OMA:   VCGICSY
- ProtClustDB:   CLSK876217
- BioCyc:   MJAN243232:MJ_0515-MONOMER
- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43639; Mature: 43508

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00535 COMPLEX1_49K; PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIH
CCCCCEECCCCCCCHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LAERVCGICSYVHTMTFAECIEHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHCCC
EHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRRDINREMMDEIYKKLDIFEDE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELK
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHCC
FKPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEA
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
PRGEVTYRMEITDGGIIKRIMIRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCT
CCCCEEEEEEECCCCHHHHHHHCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
ERCIVAVKDGKEIPISIKFR
HHEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIH
CCCCEECCCCCCCHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LAERVCGICSYVHTMTFAECIEHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHCCC
EHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRRDINREMMDEIYKKLDIFEDE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELK
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHCC
FKPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEA
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
PRGEVTYRMEITDGGIIKRIMIRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCT
CCCCEEEEEEECCCCHHHHHHHCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
ERCIVAVKDGKEIPISIKFR
HHEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087