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The map label for this gene is hycG [C]

Identifier: 15668695

GI number: 15668695

Start: 458757

End: 459203

Strand: Reverse

Name: hycG [C]

Synonym: MJ0516

Alternate gene names: 15668695

Gene position: 459203-458757 (Counterclockwise)

Preceding gene: 15668696

Following gene: 15668694

Centisome position: 27.58

GC content: 37.58

Gene sequence:

>447_bases
ATGATGAAAGAATTATTCAGAAAAAGGTCAATACATGTTTGTGTTGTCAATACTGGGGGTTGTAATGGATGCGATATTGA
GATAGTTGCCTGCTTAGCTCCAAGATACGATATTGAGCAGTATGGGATTTACGTCCATAATAACCCAAGAGAAGCGGATG
TTTTATTAGTTACAGGGCCAGTAACTTTACAATGGGCAGAGAGATTAAAGGAGATTTATGAAAAAACACCAGAACCAAAG
ATAGTTGTTGCTGTTGGAGCTTGTGCATTGAGTGGAGGGATTTTTAAGGAAGGACATGTTGTTGGAGGAGTTGATAAAGT
TATTCCTGTAGATGCAAAAATCCCTGGATGTCCTCCAAGACCTTCTGAGATTATTGAAACAATCTTAAAGGTAGCTCCTA
AGGCAATAGCAATGAGAGAAAAGAGATTAAAAAATAAAGATGAGTGA

Upstream 100 bases:

>100_bases
ATTATTCAGAAACCATAGGGCTTCGCCCTATTGGGATACCCAGGATGCATTGCTTCTGCAAAGAAGCAATGCCTCTTAAA
ATTTATTGGGAGGAATAGCC

Downstream 100 bases:

>100_bases
AAATATGGCAACAATTCCTATAGGACCAATTCATCCAGTATTGAAAGAGCCGTTAAGGATTAAACTTGTTTTAGATGGAG
AGAAACCTGTTGATGCTGAA

Product: carbon monoxide dehydrogenase hydrogenase subunit CooL

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGPVTLQWAERLKEIYEKTPEPK
IVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPRPSEIIETILKVAPKAIAMREKRLKNKDE

Sequences:

>Translated_148_residues
MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGPVTLQWAERLKEIYEKTPEPK
IVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPRPSEIIETILKVAPKAIAMREKRLKNKDE
>Mature_148_residues
MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGPVTLQWAERLKEIYEKTPEPK
IVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPRPSEIIETILKVAPKAIAMREKRLKNKDE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=128, Percent_Identity=39.0625, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789074, Length=119, Percent_Identity=49.5798319327731, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1788834, Length=129, Percent_Identity=44.9612403100775, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1788624, Length=111, Percent_Identity=39.6396396396396, Blast_Score=93, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=127, Percent_Identity=39.3700787401575, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651058, Length=127, Percent_Identity=38.5826771653543, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859983, Length=127, Percent_Identity=37.007874015748, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24642371, Length=127, Percent_Identity=37.007874015748, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y516_METJA (Q57936)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247494.1
- ProteinModelPortal:   Q57936
- SMR:   Q57936
- GeneID:   1451380
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0516
- NMPDR:   fig|243232.1.peg.531
- TIGR:   MJ0516
- HOGENOM:   HBG553221
- OMA:   NNPREAD
- ProtClustDB:   CLSK876218
- BioCyc:   MJAN243232:MJ_0516-MONOMER
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995

Pfam domain/function: PF01058 Oxidored_q6

EC number: NA

Molecular weight: Translated: 16276; Mature: 16276

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGP
CCHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECC
VTLQWAERLKEIYEKTPEPKIVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPR
EEHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCEECCCCCEEEECCCCCCCCCC
PSEIIETILKVAPKAIAMREKRLKNKDE
HHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGP
CCHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEECC
VTLQWAERLKEIYEKTPEPKIVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPR
EEHHHHHHHHHHHHHCCCCEEEEEEEEEHHCCCCCCCCCEECCCCCEEEECCCCCCCCCC
PSEIIETILKVAPKAIAMREKRLKNKDE
HHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087