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The map label for this gene is hycF [C]

Identifier: 15668691

GI number: 15668691

Start: 455134

End: 455886

Strand: Reverse

Name: hycF [C]

Synonym: MJ0514

Alternate gene names: 15668691

Gene position: 455886-455134 (Counterclockwise)

Preceding gene: 15668692

Following gene: 15668690

Centisome position: 27.38

GC content: 32.4

Gene sequence:

>753_bases
GTGATTACATTGATTGAGATAAAAAAGTCATTGGATGAGATATTATCAAAGATAGATGGGGATAAAAAGTATATTAATGA
GGTAGCCAAAAAAATAACTCCCATAACTTATAAATTGTTATATATCAACGAAACTAAATGTATTAGATGCAATCTTTGCT
ACAAAGAATGCCCAGTAGATGCAATTGAAAAAGCGAAGGTTAAAAAATCTGCAAAGATAATTGAAGATAAATGTGTTAAA
TGTGAAATTTGTGCCCAAACATGCCCTGTTGGAGCAATATATGTTATAGAGGGAAGGGCAGAGATTGAAGATAGCGAAGT
TCATTATACAATAAAAGAAAAATCAATCCCTCACAGAAAGATTAGGTTAAAAAAATATGAGCTTGATGAAAATACTTGCA
TAAAATGCGGAATTTGTGCAAGATTCTGTCCAACAAATGCTATAAAGGCAGTTAGAAGAAAGAGCATTGAGGTTAATTTA
GATTTATGTATGGGTTGTGGTGCTTGTGCTGAGGTCTGCCCAAAAAAATGTATAAAGGTTGAGAGAGAGCTTGGAGAGGT
AATAAAAACCAGAGACATTGAAGTTGATAAAAATCTATGTGTTGGATGTTTAGTTTGTATTGAAGAATGTCCTATCAACG
CAATTGATCAAGATGGAGATAAAGTTAAGATTAATAAGGATAAGTGCATATTGTGTGGAAGATGTGTAGATGTATGTCCA
ACTAATGCCATAAAGATGTGGGAAAAGAAATAA

Upstream 100 bases:

>100_bases
GAGCTTTGTGGAAAGTGTGAAGAAATTTGCCCGTTAAATGCTATAATATTACGATAAGAAATACTATTTCATAAAAAGTC
ATAATAGTTTGCATTGAAAG

Downstream 100 bases:

>100_bases
TATAATACAAGAATAAATCTTTAAAGGAAATTAATGTCTTTTAGGCATCTAAATTCCAAAGTTGATATATAAACTGCAGA
AGTCCCAATAATAAATTTAT

Product: polyferredoxin MvhB

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVDAIEKAKVKKSAKIIEDKCVK
CEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRKIRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNL
DLCMGCGACAEVCPKKCIKVERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCP
TNAIKMWEKK

Sequences:

>Translated_250_residues
MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVDAIEKAKVKKSAKIIEDKCVK
CEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRKIRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNL
DLCMGCGACAEVCPKKCIKVERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCP
TNAIKMWEKK
>Mature_250_residues
MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVDAIEKAKVKKSAKIIEDKCVK
CEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRKIRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNL
DLCMGCGACAEVCPKKCIKVERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCP
TNAIKMWEKK

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 4Fe-4S ferredoxin-type domains

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y514_METJA (Q57934)

Other databases:

- EMBL:   L77117
- PIR:   B64364
- RefSeq:   NP_247490.1
- ProteinModelPortal:   Q57934
- SMR:   Q57934
- GeneID:   1451376
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0514
- NMPDR:   fig|243232.1.peg.527
- TIGR:   MJ0514
- HOGENOM:   HBG551655
- OMA:   CIRCNLC
- ProtClustDB:   CLSK876214
- BioCyc:   MJAN243232:MJ_0514-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 28246; Mature: 28246

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
11.2 %Cys+Met (Translated Protein)
10.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
11.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVD
CEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCHHHCCHH
AIEKAKVKKSAKIIEDKCVKCEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRK
HHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHH
IRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNLDLCMGCGACAEVCPKKCIKV
EEEEEEECCCCCEEEECCHHHHCCHHHHHHHHHCCCEEEEHEEECCCHHHHHHHHHHHHH
ERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCHHHHCC
TNAIKMWEKK
CCHHHHCCCC
>Mature Secondary Structure
MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVD
CEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCHHHCCHH
AIEKAKVKKSAKIIEDKCVKCEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRK
HHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHH
IRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNLDLCMGCGACAEVCPKKCIKV
EEEEEEECCCCCEEEECCHHHHCCHHHHHHHHHCCCEEEEHEEECCCHHHHHHHHHHHHH
ERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCHHHHCC
TNAIKMWEKK
CCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087