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The map label for this gene is hycF [C]

Identifier: 15668692

GI number: 15668692

Start: 455930

End: 456421

Strand: Reverse

Name: hycF [C]

Synonym: MJ0514.1

Alternate gene names: 15668692

Gene position: 456421-455930 (Counterclockwise)

Preceding gene: 15668693

Following gene: 15668691

Centisome position: 27.41

GC content: 27.85

Gene sequence:

>492_bases
ATGATAAAGGAAATAATAGCTAAACATTTCAATTTAGCTGATAAAAATATCCAATTACTCCCAAAATTTAATATTATTTT
AAATAAAAGAGAGATTATCGTTAAAGAGGATAAATGCATTAGCTGTGGAAAATGTATTGAAATCTGCCCAGTGAATGCAA
TAACCTACAGTAGTGATGGGTTATATATAACTATTAATAAAGAAAAATGTGTGTTTTGTGGAAAATGCAAAAAAGTTTGT
CCAACAAATGCAATTGTAATAATAAGATTGAGATGCGAAATTAACGAAGATGCAAGGATTATTGAAGTAGATAAGTATGA
ATTTATTGATTATATAAGTGAGAGATGTGCATCTTGCTTAGTTTGTTTAAGGAATTGCCCATTTAATGCTATTGAAGAAT
ATGGAAGTAAAATAAGGATTGATATAAATAAGTGTGAGCTTTGTGGAAAGTGTGAAGAAATTTGCCCGTTAAATGCTATA
ATATTACGATAA

Upstream 100 bases:

>100_bases
GAAGAGATTGAAAAGTTATCCCTCAAAAAGTTGAAAGAAGAGCTAAAAAGGAGAGTTTATGAAGAAAATAAAAGAATAAT
GGAAAAAAAGAGGGAGCTGT

Downstream 100 bases:

>100_bases
GAAATACTATTTCATAAAAAGTCATAATAGTTTGCATTGAAAGGTGATTACATTGATTGAGATAAAAAAGTCATTGGATG
AGATATTATCAAAGATAGAT

Product: polyferredoxin MvhB

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MIKEIIAKHFNLADKNIQLLPKFNIILNKREIIVKEDKCISCGKCIEICPVNAITYSSDGLYITINKEKCVFCGKCKKVC
PTNAIVIIRLRCEINEDARIIEVDKYEFIDYISERCASCLVCLRNCPFNAIEEYGSKIRIDINKCELCGKCEEICPLNAI
ILR

Sequences:

>Translated_163_residues
MIKEIIAKHFNLADKNIQLLPKFNIILNKREIIVKEDKCISCGKCIEICPVNAITYSSDGLYITINKEKCVFCGKCKKVC
PTNAIVIIRLRCEINEDARIIEVDKYEFIDYISERCASCLVCLRNCPFNAIEEYGSKIRIDINKCELCGKCEEICPLNAI
ILR
>Mature_163_residues
MIKEIIAKHFNLADKNIQLLPKFNIILNKREIIVKEDKCISCGKCIEICPVNAITYSSDGLYITINKEKCVFCGKCKKVC
PTNAIVIIRLRCEINEDARIIEVDKYEFIDYISERCASCLVCLRNCPFNAIEEYGSKIRIDINKCELCGKCEEICPLNAI
ILR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y51A_METJA (P81292)

Other databases:

- EMBL:   L77117
- RefSeq:   NP_247491.1
- ProteinModelPortal:   P81292
- GeneID:   1451377
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0514.1
- NMPDR:   fig|243232.1.peg.528
- TIGR:   MJ0514.1
- HOGENOM:   HBG541111
- OMA:   VLCGHCE
- ProtClustDB:   CLSK876215
- BioCyc:   MJAN243232:MJ_0514.1-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 18645; Mature: 18645

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
11.0 %Cys+Met (Translated Protein)
10.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
11.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEIIAKHFNLADKNIQLLPKFNIILNKREIIVKEDKCISCGKCIEICPVNAITYSSDG
CHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEECCCC
LYITINKEKCVFCGKCKKVCPTNAIVIIRLRCEINEDARIIEVDKYEFIDYISERCASCL
EEEEECCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHH
VCLRNCPFNAIEEYGSKIRIDINKCELCGKCEEICPLNAIILR
HHHHCCCCHHHHHCCCEEEEEEHHCCCCCCHHHHCCCEEEEEC
>Mature Secondary Structure
MIKEIIAKHFNLADKNIQLLPKFNIILNKREIIVKEDKCISCGKCIEICPVNAITYSSDG
CHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEECCCC
LYITINKEKCVFCGKCKKVCPTNAIVIIRLRCEINEDARIIEVDKYEFIDYISERCASCL
EEEEECCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHH
VCLRNCPFNAIEEYGSKIRIDINKCELCGKCEEICPLNAIILR
HHHHCCCCHHHHHCCCEEEEEEHHCCCCCCHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087