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The map label for this gene is lysS [H]

Identifier: 16273131

GI number: 16273131

Start: 1277530

End: 1279038

Strand: Reverse

Name: lysS [H]

Synonym: HI1211

Alternate gene names: 16273131

Gene position: 1279038-1277530 (Counterclockwise)

Preceding gene: 16273136

Following gene: 16273129

Centisome position: 69.89

GC content: 39.63

Gene sequence:

>1509_bases
ATGTCAGAACAAGAAGTTAAAGAATTAGATCTCAATGGCGAAATGCTCGTCCGTCGTGAAAAATTAGCCGCATTACGTGC
AAAAGGTAATGCCTTTCCAAATAAGTTTCGTCGTGATGCTCTCGCACAAGATTTGCACAATCAATATGATGCAGAAGACG
GGGAAATCTTAAAAGAAAAAGGCATTGAAGTACAAGTTGCTGGTCGTATTATGACTCGCCGTGCAATGGGTAAAGCAACT
TTTATCACTATTCAAGATATGAGCGGTAAAATCCAACTTTATGTTGCACGCGATAATTTGCCTGAAGGTGTTTATAAAGA
TGACGTTGGTACTTGGGATTTAGGTGATATCGTTGGCATTAAAGGCACTTTATTTAAAACAAAAACTGATGAACTCACCG
TTAAAACTACGGAAGTTCAACTTTTAACCAAAGCACTTCGCCCATTACCAGACAAATTCCATGGTTTAACCGACCAAGAA
GTTCGTTATCGCCAACGTTATTTAGATTTAATTTCTAACGAAGAATCTCGCCGCACTTTTATTATTCGTTCTAAAGTTGT
CGCGGGTATTCGTGAATATTTCATTTCTAAAGGCTTTATGGAAGTGGAAACGCCAATGTTACAAGTGATTCCAGGCGGTG
CATCTGCGCGTCCTTTCGTGACTCATCACAATGCATTAGATGTGGATATGTATTTACGTATCGCACCTGAACTTTATTTA
AAACGCTTAGTCGTTGGTGGGTTTGAACGAGTATTTGAATTAAACCGTAACTTCCGTAATGAAGGGGTTTCTGTTCGTCA
TAATCCAGAATTTACTATGCTTGAATACTATCAAGCATATGCGGACTACCATGATTTAATGGATAACACCGAAGAACTTC
TTCGTAAATTAGCGATTGATATTCTTGGTACAACCATTGTGAAATACGGTGATTTAGAATTTGACTTCGGCAAACCATTT
GAGCGTATTACATTACATGATGCAACCATTAAATATGGTGCAGATAAAGGTATCGTAAAAGAAGATCTTTATGACTTCGA
TCGTGCAAAAGCAACTGCTGAACGCTTAGGTATTGAAGTACAAAAATCTTGGGGTTTAGGCAGCATTGTCAATGCAATCT
TTGAAGAAGTGGCTGAACATCACTTAATTCAACCAACCTTTCTTAATGGCTCACCTGCGGAAATTTCGCCACTTGCACGT
CGTAATGATGAAAACCCTGAAGTGACTGACCGTTTTGAATTATTCATCGGCGGACGTGAAATTGGTAACGGTTTCTCAGA
ATTAAATGACGCGGAAGATCAAAATGAACGCTTTGATGCACAAGTGGCTGCGAAAGAAGCGGGCGATGATGAAGCGATGT
TTAAAGATGAAGACTTTGTTGTTGCACTAGAACACGGCTTACCACCAACAGCTGGGGAAGGTTTAGGTATCGACCGTCTA
GCGATGCTTTATGCAAATGCACCGTCAATTCGTGATGTTATCTTATTCCCTGCAATGCGTCAGAAATAA

Upstream 100 bases:

>100_bases
AGGGGGCTTTCGATAATATATTTCCTTTAGTCTTCGACAGCTCCTAAAGTAAACAAAGAAAATAAAAATTTCTTATTAAT
TTATAAAAAAGGTAAACAAT

Downstream 100 bases:

>100_bases
TAAAGATATAAACAAAAGCCACCATTTGGTGGCTTTTTTATCGAAACATAAAAAGAGAGCTAACTCCCCCTTATTTTTTA
ATGATTAACCATTAATAAAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDALAQDLHNQYDAEDGEILKEKGIEVQVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVARDNLPEGVYKDDVGTWDLGDIVGIKGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQE
VRYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPF
ERITLHDATIKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLNGSPAEISPLAR
RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
AMLYANAPSIRDVILFPAMRQK

Sequences:

>Translated_502_residues
MSEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDALAQDLHNQYDAEDGEILKEKGIEVQVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVARDNLPEGVYKDDVGTWDLGDIVGIKGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQE
VRYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPF
ERITLHDATIKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLNGSPAEISPLAR
RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
AMLYANAPSIRDVILFPAMRQK
>Mature_501_residues
SEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDALAQDLHNQYDAEDGEILKEKGIEVQVAGRIMTRRAMGKATF
ITIQDMSGKIQLYVARDNLPEGVYKDDVGTWDLGDIVGIKGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQEV
RYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYLK
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPFE
RITLHDATIKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLNGSPAEISPLARR
NDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLA
MLYANAPSIRDVILFPAMRQK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=511, Percent_Identity=40.9001956947162, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI5031815, Length=511, Percent_Identity=40.9001956947162, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI45439306, Length=465, Percent_Identity=23.8709677419355, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI40789249, Length=289, Percent_Identity=27.3356401384083, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1790571, Length=504, Percent_Identity=69.8412698412698, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=69.6428571428571, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=31.8042813455657, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788173, Length=288, Percent_Identity=25.6944444444444, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=507, Percent_Identity=41.6173570019724, Blast_Score=357, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI17535927, Length=507, Percent_Identity=41.2228796844181, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=505, Percent_Identity=41.5841584158416, Blast_Score=357, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI17551876, Length=473, Percent_Identity=23.4672304439746, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=287, Percent_Identity=25.0871080139373, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=516, Percent_Identity=40.1162790697674, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324256, Length=386, Percent_Identity=30.8290155440415, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=481, Percent_Identity=25.3638253638254, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640849, Length=489, Percent_Identity=41.3087934560327, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=41.3087934560327, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136276, Length=465, Percent_Identity=23.8709677419355, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24584738, Length=236, Percent_Identity=31.3559322033898, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56936; Mature: 56805

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDALAQDLHNQYDAEDGEILKEK
CCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GIEVQVAGRIMTRRAMGKATFITIQDMSGKIQLYVARDNLPEGVYKDDVGTWDLGDIVGI
CCEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQEVRYRQRYLDLISNEESRRTF
CCEEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
IIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
EHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTIVKYGDLEFDFGKPFERITLHDATIKYGADKGIVKEDLYDFDRAKATAERLGIEV
HHHHHHHHCCCEEECCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEE
QKSWGLGSIVNAIFEEVAEHHLIQPTFLNGSPAEISPLARRNDENPEVTDRFELFIGGRE
ECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHCCCCCCCCCCEEEEEECCHH
IGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHH
AMLYANAPSIRDVILFPAMRQK
HHHHCCCCCHHHEEECCCCCCC
>Mature Secondary Structure 
SEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDALAQDLHNQYDAEDGEILKEK
CHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GIEVQVAGRIMTRRAMGKATFITIQDMSGKIQLYVARDNLPEGVYKDDVGTWDLGDIVGI
CCEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
KGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQEVRYRQRYLDLISNEESRRTF
CCEEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
IIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
EHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTIVKYGDLEFDFGKPFERITLHDATIKYGADKGIVKEDLYDFDRAKATAERLGIEV
HHHHHHHHCCCEEECCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEE
QKSWGLGSIVNAIFEEVAEHHLIQPTFLNGSPAEISPLARRNDENPEVTDRFELFIGGRE
ECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHCCCCCCCCCCEEEEEECCHH
IGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHH
AMLYANAPSIRDVILFPAMRQK
HHHHCCCCCHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA