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The map label for this gene is queD

Identifier: 16273112

GI number: 16273112

Start: 1257152

End: 1257577

Strand: Reverse

Name: queD

Synonym: HI1190

Alternate gene names: 16273112

Gene position: 1257577-1257152 (Counterclockwise)

Preceding gene: 16273113

Following gene: 16273111

Centisome position: 68.71

GC content: 32.86

Gene sequence:

>426_bases
ATGTTTAAAATTTCCAAAGAATTTAGCTTTGACATGGCACATTTATTAGATGGACATGATGGGAAATGTCAAAATTTACA
TGGACACACATATAAATTACAAGTTGAAATTTCGGGTGACTTATATAAATCAGGTGCAAAAAAAGCAATGGTCATTGATT
TTTCAGATCTAAAGTCTATTGTGAAAAAAGTTATTTTAGATCCGATGGATCACGCCTTTATTTATGATCAAACCAATGAG
CGAGAAAGCCAAATTGCCACTCTATTGCAAAAGTTAAACTCAAAAACTTTTGGTGTTCCCTTTAGAACTACGGCAGAAGA
AATAGCTCGTTTTATTTTTAATCGTTTAAAACATGATGAACAACTGTCCATATCATCCATTCGTTTATGGGAAACCCCAA
CTTCATTTTGCGAGTATCAGGAGTAA

Upstream 100 bases:

>100_bases
ACTTGCTATGAAGGCATCGAAGGTGGATGCAGAAAATGCCCAAGCTGTATTTTACGCAATAAAGGTTTAAAGAAATATTT
GACTCAAAAAGGTAGAAAAA

Downstream 100 bases:

>100_bases
ATGTTGATGGAAAAATATCATGATTTTGAACCGAACTTTAACATAGTCGAAATTTTTGAAAGTCTGCAAGGGGAGGGCTT
CAATACGGGAATGCCAAGTG

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine; Triphosphate. [C]

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MFKISKEFSFDMAHLLDGHDGKCQNLHGHTYKLQVEISGDLYKSGAKKAMVIDFSDLKSIVKKVILDPMDHAFIYDQTNE
RESQIATLLQKLNSKTFGVPFRTTAEEIARFIFNRLKHDEQLSISSIRLWETPTSFCEYQE

Sequences:

>Translated_141_residues
MFKISKEFSFDMAHLLDGHDGKCQNLHGHTYKLQVEISGDLYKSGAKKAMVIDFSDLKSIVKKVILDPMDHAFIYDQTNE
RESQIATLLQKLNSKTFGVPFRTTAEEIARFIFNRLKHDEQLSISSIRLWETPTSFCEYQE
>Mature_141_residues
MFKISKEFSFDMAHLLDGHDGKCQNLHGHTYKLQVEISGDLYKSGAKKAMVIDFSDLKSIVKKVILDPMDHAFIYDQTNE
RESQIATLLQKLNSKTFGVPFRTTAEEIARFIFNRLKHDEQLSISSIRLWETPTSFCEYQE

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUED_HAEIN (P44123)

Other databases:

- EMBL:   L42023
- PIR:   F64021
- RefSeq:   NP_439346.1
- ProteinModelPortal:   P44123
- SMR:   P44123
- GeneID:   950142
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1190
- NMPDR:   fig|71421.1.peg.1137
- TIGR:   HI_1190
- HOGENOM:   HBG727155
- OMA:   HITDPFD
- ProtClustDB:   CLSK870004
- BioCyc:   HINF71421:HI_1190-MONOMER
- BRENDA:   4.2.3.12
- InterPro:   IPR007116
- InterPro:   IPR007115
- PANTHER:   PTHR12589
- TIGRFAMs:   TIGR00039

Pfam domain/function: PF01242 PTPS

EC number: 4.2.3.12 [C]

Molecular weight: Translated: 16322; Mature: 16322

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 23-23 ACT_SITE 71-71 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKISKEFSFDMAHLLDGHDGKCQNLHGHTYKLQVEISGDLYKSGAKKAMVIDFSDLKSI
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHCCCCEEEEEEHHHHHHH
VKKVILDPMDHAFIYDQTNERESQIATLLQKLNSKTFGVPFRTTAEEIARFIFNRLKHDE
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
QLSISSIRLWETPTSFCEYQE
CCCCCEEEEECCCHHHCCCCC
>Mature Secondary Structure
MFKISKEFSFDMAHLLDGHDGKCQNLHGHTYKLQVEISGDLYKSGAKKAMVIDFSDLKSI
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHCCCCEEEEEEHHHHHHH
VKKVILDPMDHAFIYDQTNERESQIATLLQKLNSKTFGVPFRTTAEEIARFIFNRLKHDE
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
QLSISSIRLWETPTSFCEYQE
CCCCCEEEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate [C]

Specific reaction: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate = 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine + Triphosphate. [C]

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800