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The map label for this gene is cpdB [H]

Identifier: 99080877

GI number: 99080877

Start: 1115617

End: 1117575

Strand: Reverse

Name: cpdB [H]

Synonym: TM1040_1036

Alternate gene names: 99080877

Gene position: 1117575-1115617 (Counterclockwise)

Preceding gene: 99080878

Following gene: 99080866

Centisome position: 34.91

GC content: 60.69

Gene sequence:

>1959_bases
ATGTCGTGGCAGATTAATCGTAGATCCTTTCTCGCAGGCAGCGCGGGCCTTATTGCGCTACATCCGTTTTCGGTGGCTGC
GGCCTCGGGGCAGGTTCACCTGCGCCTGATGGAAACAACGGATCTGCACGTCCATGTGTTTCCCTATGACTATTATGGCG
ACAAACCCGTGGACACCCTGGGCCTCGCCCGCACCGCATCGCTGATCAACGACATCCGCGCCGAGGCCACAAACTCGCTC
TTGGTGGACAATGGGGATTTCCTGCAGGGCAACCCGATGGGCGACTACATCGCCTATGAGCGTGGCATGAAAGAAGGCGA
CCAGCACCCGGTGATCACCGCGATGAACACCGTCGGCTTTGATGCCTCGACCTTGGGCAACCACGAATTCAACTACGGGA
TTTCCTTTTTGATGAAATCCCTTGCGGGCGCCGGGTTCCCGGTGGTTTGTGCCAATGTCGCCAAGAAAACCGGTGCCTCA
CCGCGCGAGGATGAGACCCTGTTGCCGCCCTATGTGATTCTGGAGCGCGAACTCACCGATGGCGCGGGCAAGGCGCACCC
GATCAAGATCGGCCTCATCGGATTTGTGCCGCCGCAGGTGATGAACTGGGACCGCAAACATCTTGAAGGCAACGTGCAGG
CGCGCGACATCGTCGAATGCGCCCGTGCCTATGTGCCGGAGATGAAGGAGAAAGGCGCAGATATCATCATCGCGCTCTCG
CATTCCGGCATCGGCTCGGCCGATCACAGTGACGGCATGGAAAATGCCTCGGTGCCGCTGGCGGCAGTAGAGGGCATTGA
CGCCATCATGACCGGTCACAGCCATCTGGTGTTCCCCTCCTCGACCTATGCGGATTTTGCAGGCGTTGACGCCGACAAAG
GCACGATCCACGGCACGCCCGCCGTCATGGGCGGCTATTGGGGCAGCCATATGGGGCTCATTGACCTGATGCTGGAGCGC
GATGGCAATGGCTGGCGCGTTGTGGGCCATGCCTCCGAGGCGCGCCCGATTTCCAAGCGCAACGAGGATCGCAGCGTGAC
CGCACTGGTCGAGAGCGATCAGAGCGTGCTGGAGGCGGTGCAGGCCGATCACGACGCCACGCTTGCTTATGTGCGCCGCG
CGGTGGGCAAGACTGATGCACCGCTGCACAGCTATTTTGCGCTGGTGGCAGATGACCCTTCGGTGCAGATCGTGTCGATT
GCACAGACCTGGTATATCTCTCAGATGCTGAAGGGCACCGAGTATGAGGCTCTGCCGATCCTCTCTGCCGCCGCCCCTTT
CAAGGCAGGTGGCCGAGGTGGTCCGGAGTATTACACCGATGTTCCGGTGGGGGATGTGGCAATCAAGAATGTCGCCGATC
TCTACCTCTATCCCAACACCGTACGCGCCGTGAAAGTCACCGGCCAGCAGGTGAAGGACTGGCTTGAGCGCTCGGCGGGC
ATGTTCAACCAGATCGAGCCCGGCAAGGCCGACCAGGTGCTGCTCAACCCGTCGTTCCCCAGCTACAACTTCGATGTGAT
CGACGGCGTGACCTATCAGATTGACCTGAGCCAGCCGCCGATGTTCGCGCCCAAGGGCGAGCTGATCAACCCGGATAGCA
ACCGGATCGTGAACCTTGAGTTCAACGGAGCCCCCATTGATCCCGCGCAGGAGTTCATCATTGCCACCAATAACTACCGT
GCCAGCGGCGGTGGCAGCTTCCCCGGTGCGATGGGTGATACCATCGTCTTTGAAGGGCCAGACACCAACCGCGATGTGAT
CGTGCGCTATATCGTCGAACAGGGCACCATCAGCCCTAAGGCGGATGGCAACTGGAGCTTTGCCCCGCTGCCGGACACCT
CGGTTCTGTTTGACACCGGCCCCAAAGCCGCCGCCTATGCCGAGAGCGTCCCCGGTGTTACCATCGCACCTGCAGGGGAT
GGGCCGGACGGGTTTGCCCGCTTCAAGATCACGCTCTGA

Upstream 100 bases:

>100_bases
AACCCATGACAAACTGTCACAGTATAGCGCTATCATAAGTGGAATCGCGCAGCTGTCGCTCGACGCATGCGTGACGATCT
CAAGACTTCAGGAGAGTTAA

Downstream 100 bases:

>100_bases
GCGAAAAACGGCATCGCGCCGCCCAGCATTGGACTGGGCGGCGTTTTTCTTTTGGTGGCGTTTTCCACTGGGCGACAGGG
CAACCGGGCAACCGGGAGAC

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MSWQINRRSFLAGSAGLIALHPFSVAAASGQVHLRLMETTDLHVHVFPYDYYGDKPVDTLGLARTASLINDIRAEATNSL
LVDNGDFLQGNPMGDYIAYERGMKEGDQHPVITAMNTVGFDASTLGNHEFNYGISFLMKSLAGAGFPVVCANVAKKTGAS
PREDETLLPPYVILERELTDGAGKAHPIKIGLIGFVPPQVMNWDRKHLEGNVQARDIVECARAYVPEMKEKGADIIIALS
HSGIGSADHSDGMENASVPLAAVEGIDAIMTGHSHLVFPSSTYADFAGVDADKGTIHGTPAVMGGYWGSHMGLIDLMLER
DGNGWRVVGHASEARPISKRNEDRSVTALVESDQSVLEAVQADHDATLAYVRRAVGKTDAPLHSYFALVADDPSVQIVSI
AQTWYISQMLKGTEYEALPILSAAAPFKAGGRGGPEYYTDVPVGDVAIKNVADLYLYPNTVRAVKVTGQQVKDWLERSAG
MFNQIEPGKADQVLLNPSFPSYNFDVIDGVTYQIDLSQPPMFAPKGELINPDSNRIVNLEFNGAPIDPAQEFIIATNNYR
ASGGGSFPGAMGDTIVFEGPDTNRDVIVRYIVEQGTISPKADGNWSFAPLPDTSVLFDTGPKAAAYAESVPGVTIAPAGD
GPDGFARFKITL

Sequences:

>Translated_652_residues
MSWQINRRSFLAGSAGLIALHPFSVAAASGQVHLRLMETTDLHVHVFPYDYYGDKPVDTLGLARTASLINDIRAEATNSL
LVDNGDFLQGNPMGDYIAYERGMKEGDQHPVITAMNTVGFDASTLGNHEFNYGISFLMKSLAGAGFPVVCANVAKKTGAS
PREDETLLPPYVILERELTDGAGKAHPIKIGLIGFVPPQVMNWDRKHLEGNVQARDIVECARAYVPEMKEKGADIIIALS
HSGIGSADHSDGMENASVPLAAVEGIDAIMTGHSHLVFPSSTYADFAGVDADKGTIHGTPAVMGGYWGSHMGLIDLMLER
DGNGWRVVGHASEARPISKRNEDRSVTALVESDQSVLEAVQADHDATLAYVRRAVGKTDAPLHSYFALVADDPSVQIVSI
AQTWYISQMLKGTEYEALPILSAAAPFKAGGRGGPEYYTDVPVGDVAIKNVADLYLYPNTVRAVKVTGQQVKDWLERSAG
MFNQIEPGKADQVLLNPSFPSYNFDVIDGVTYQIDLSQPPMFAPKGELINPDSNRIVNLEFNGAPIDPAQEFIIATNNYR
ASGGGSFPGAMGDTIVFEGPDTNRDVIVRYIVEQGTISPKADGNWSFAPLPDTSVLFDTGPKAAAYAESVPGVTIAPAGD
GPDGFARFKITL
>Mature_651_residues
SWQINRRSFLAGSAGLIALHPFSVAAASGQVHLRLMETTDLHVHVFPYDYYGDKPVDTLGLARTASLINDIRAEATNSLL
VDNGDFLQGNPMGDYIAYERGMKEGDQHPVITAMNTVGFDASTLGNHEFNYGISFLMKSLAGAGFPVVCANVAKKTGASP
REDETLLPPYVILERELTDGAGKAHPIKIGLIGFVPPQVMNWDRKHLEGNVQARDIVECARAYVPEMKEKGADIIIALSH
SGIGSADHSDGMENASVPLAAVEGIDAIMTGHSHLVFPSSTYADFAGVDADKGTIHGTPAVMGGYWGSHMGLIDLMLERD
GNGWRVVGHASEARPISKRNEDRSVTALVESDQSVLEAVQADHDATLAYVRRAVGKTDAPLHSYFALVADDPSVQIVSIA
QTWYISQMLKGTEYEALPILSAAAPFKAGGRGGPEYYTDVPVGDVAIKNVADLYLYPNTVRAVKVTGQQVKDWLERSAGM
FNQIEPGKADQVLLNPSFPSYNFDVIDGVTYQIDLSQPPMFAPKGELINPDSNRIVNLEFNGAPIDPAQEFIIATNNYRA
SGGGSFPGAMGDTIVFEGPDTNRDVIVRYIVEQGTISPKADGNWSFAPLPDTSVLFDTGPKAAAYAESVPGVTIAPAGDG
PDGFARFKITL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=653, Percent_Identity=46.5543644716692, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1786687, Length=592, Percent_Identity=23.1418918918919, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 69985; Mature: 69854

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQINRRSFLAGSAGLIALHPFSVAAASGQVHLRLMETTDLHVHVFPYDYYGDKPVDTL
CCCCCCCCEEECCCCCEEEECCCEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCHHH
GLARTASLINDIRAEATNSLLVDNGDFLQGNPMGDYIAYERGMKEGDQHPVITAMNTVGF
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCHHHHHHCCCCCCCCCEEEEEEECCC
DASTLGNHEFNYGISFLMKSLAGAGFPVVCANVAKKTGASPREDETLLPPYVILERELTD
CHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
GAGKAHPIKIGLIGFVPPQVMNWDRKHLEGNVQARDIVECARAYVPEMKEKGADIIIALS
CCCCCCEEEEEEEECCCHHHHCCCHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEEE
HSGIGSADHSDGMENASVPLAAVEGIDAIMTGHSHLVFPSSTYADFAGVDADKGTIHGTP
CCCCCCCCCCCCCCCCCCCCHHHCCCHHEECCCCEEEECCCCCCHHCCCCCCCCEECCCC
AVMGGYWGSHMGLIDLMLERDGNGWRVVGHASEARPISKRNEDRSVTALVESDQSVLEAV
HHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
QADHDATLAYVRRAVGKTDAPLHSYFALVADDPSVQIVSIAQTWYISQMLKGTEYEALPI
HCCCCHHHHHHHHHHCCCCCCHHHEEEEEECCCCEEEEEEHHHHHHHHHHCCCCCCEEEE
LSAAAPFKAGGRGGPEYYTDVPVGDVAIKNVADLYLYPNTVRAVKVTGQQVKDWLERSAG
EECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHCC
MFNQIEPGKADQVLLNPSFPSYNFDVIDGVTYQIDLSQPPMFAPKGELINPDSNRIVNLE
CCCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCCCEECCCCCEEEEEE
FNGAPIDPAQEFIIATNNYRASGGGSFPGAMGDTIVFEGPDTNRDVIVRYIVEQGTISPK
ECCCCCCCCCEEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
ADGNWSFAPLPDTSVLFDTGPKAAAYAESVPGVTIAPAGDGPDGFARFKITL
CCCCEEECCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEEC
>Mature Secondary Structure 
SWQINRRSFLAGSAGLIALHPFSVAAASGQVHLRLMETTDLHVHVFPYDYYGDKPVDTL
CCCCCCCEEECCCCCEEEECCCEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCHHH
GLARTASLINDIRAEATNSLLVDNGDFLQGNPMGDYIAYERGMKEGDQHPVITAMNTVGF
HHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCHHHHHHCCCCCCCCCEEEEEEECCC
DASTLGNHEFNYGISFLMKSLAGAGFPVVCANVAKKTGASPREDETLLPPYVILERELTD
CHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCC
GAGKAHPIKIGLIGFVPPQVMNWDRKHLEGNVQARDIVECARAYVPEMKEKGADIIIALS
CCCCCCEEEEEEEECCCHHHHCCCHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCEEEEEE
HSGIGSADHSDGMENASVPLAAVEGIDAIMTGHSHLVFPSSTYADFAGVDADKGTIHGTP
CCCCCCCCCCCCCCCCCCCCHHHCCCHHEECCCCEEEECCCCCCHHCCCCCCCCEECCCC
AVMGGYWGSHMGLIDLMLERDGNGWRVVGHASEARPISKRNEDRSVTALVESDQSVLEAV
HHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
QADHDATLAYVRRAVGKTDAPLHSYFALVADDPSVQIVSIAQTWYISQMLKGTEYEALPI
HCCCCHHHHHHHHHHCCCCCCHHHEEEEEECCCCEEEEEEHHHHHHHHHHCCCCCCEEEE
LSAAAPFKAGGRGGPEYYTDVPVGDVAIKNVADLYLYPNTVRAVKVTGQQVKDWLERSAG
EECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHCC
MFNQIEPGKADQVLLNPSFPSYNFDVIDGVTYQIDLSQPPMFAPKGELINPDSNRIVNLE
CCCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCCCEECCCCCEEEEEE
FNGAPIDPAQEFIIATNNYRASGGGSFPGAMGDTIVFEGPDTNRDVIVRYIVEQGTISPK
ECCCCCCCCCEEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
ADGNWSFAPLPDTSVLFDTGPKAAAYAESVPGVTIAPAGDGPDGFARFKITL
CCCCEEECCCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]