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The map label for this gene is acnB [H]

Identifier: 99080867

GI number: 99080867

Start: 1097776

End: 1100565

Strand: Direct

Name: acnB [H]

Synonym: TM1040_1026

Alternate gene names: 99080867

Gene position: 1097776-1100565 (Clockwise)

Preceding gene: 99080865

Following gene: 99080868

Centisome position: 34.3

GC content: 59.43

Gene sequence:

>2790_bases
ATGAGCTTGTACACAGACTACCTAAATGAGATCGAAACCCGCAAAGGCGAGGGGCTGCACCCCAAGCCCATCGACGATGG
CGCGCTGGTTGCGGAATTGATTGCGCAGATCAAGGACACCGGGAGCGAGCATCGTGAAGACTCTCTGAAGTTCTTCATTT
ACAACACCTTGCCCGGCACCACGAGCGCTGCAGGTGTCAAGGCGGCGTTTCTCAAAGAGATCATTCTGGGCGAGGCCGAG
GTGGCTGAAATCACGCCCGAGTTCGCCTTTGAACTGCTGTCGCACATGAAGGGTGGTCCCTCGGTCGAGGTGCTTCTGGA
TCTGGCCCTCGGTGAGGATGCAGGGATCGCCCAGCAGGCCGCAGACGTGCTGAAGACACAGGTGTTCCTCTATGAGGCGG
ACACCGATCGTCTCGAAGCAGCTTACAAGGCTGGCAATGCGATTGCGCGTGAGATCCTCGAGAGCTACGCCAAGGCCGAG
TTCTTTACCAAGCTTCCCGATGTGGATGATGAGATCAAGGTCGTGACCTACATCGCCGCCGAAGGCGATATCTCCACCGA
CCTTCTGTCCCCGGGCAATCAGGCGCACTCGCGTTCGGATCGGGAACTGCATGGCAAATGCATGATCTCCGAGGCGGCGC
AAAAGGAGATCGAGGCGCTGAAGCTGCAGCACCCCGACAAGCGCGTGATGCTGATCGCGGAAAAAGGCACCATGGGCGTG
GGCTCCTCGCGGATGTCCGGCGTCAACAACGTGGCGCTCTGGACCGGCAAACAAGCCAGCCCCTATGTGCCGTTTGTGAA
CTATGCCCCTGTGGTTGCGGGCACCAATGGCATCTCGCCGATTTTCCTCACCACCGTGGGCGTGACCGGCGGCATTGGCC
TCGACCTCAAGAACTGGGTCAAGAAGGTCGATAGCGACGGCAAGGCGATCCTCAACAACGACGGCAACCCGGTGCTGGAG
CAGAAGTATTCTGTTGAAACCGGCACGGTGCTGAAGATCGACACCAAGAACAAGAAGCTCCTGAGCGAAGACGGCGAGGA
GCTGGTGGATGTCTCGTCCGCGTTTACGCCCCAGAAGGTCGAGTTCATGAAGGCGGGCAGCTCTTATGCCATCGTCTTTG
GCAAGAAACTGCAGACATTTGCGGCCGAGACCCTCGGCATCGAGGCGCCGCTGGTCTTTGCACCGGCGAAAGAAGTCAGC
CACGAGGGCCAGGGCCTGACTGCGGTTGAAAAGATCTTTAACCGCAATGCCGTGGGCAGCACGCCGGGCAAGACGCTCCA
TGCGGGCTCTGACGTGCGCGTGAAGGTCAACATTGTCGGCTCTCAGGACACCACTGGTCTCATGACCTCGCAAGAACTGG
AGGCCATGGCGGCCACCGTGATCTCGCCGACCGTGGATGGGGCCTATCAGTCCGGCTGTCACACCGCGTCCGTGTGGGAC
AAGAAGGCACAGGCCAACATTCCGCGTCTGATGAAGTTCATGAACAACTTCGGCCTGATCACCGCGCGCGATCCAAAGGA
TCAGTATCACGCGATGACCGACGTGATCCATAAGGTGCTGAACGACCTCACCGTGGATGACTGGGACATCATCATCGGCG
GCGACAGCCACACCCGCATGTCCAAAGGCGTGGCCTTTGGTGCGGACTCGGGTACCGTGGCGCTGGCGCTGGCAACCGGT
GAGGCGACCATGCCAATCCCCGAGTCGGTCAAAGTGACCTTCAAGGGCAAGATGGCCGACCATATGGACTTCCGCGATGT
GGTTCACGCCACCCAGGCGCAGATGCTCAAGCAGCATGGCGACAACGTCTTCCAGGGCCGCGTCATCGAGGTCCACATCG
GCACGCTCCTGGCAGACCAGGCCTTTACCTTCACCGACTGGACGGCCGAGATGAAGGCCAAGGCGTCGATCTGTATCTCC
AATGACGATACGCTCATCGGCTCGCTCGAGCTCGCCAAGAGCCGCATCCAGATCATGATCGACAAAGGCATGGACAACGA
GGCGGGTACCCTCCAGGGGCTGATCGACAAGGCCGACAAGCGCATCGCCGAGATCCGCTCCGGTGAGAAGCCGGCCCTGT
CGCCCGACGACAGCGCCAAGTATTACGCCGAAGTGGTGGTCGATCTCGACGAGATCAACGAGCCGATGATTGCCGACCCG
GACGTCAACAACGAGGACGTCTCCAAGCGCTACACCCACGACACCATCCGTCCGATCTCTTTCTATAACGCAGAGAAAAA
GGTCGATCTTGGCTTTGTGGGCTCCTGCATGGTGCACAAGGGAGACATGAAGATCGTGGCGCAGATGCTCAAGAACCTCG
AGAAAGCCAACGGCAAGGTCGAGTTCAAGGCTCCGCTCGTGGTGGCCGCGCCGACCTACAACATCATCGACGAGATGAAG
GCCGAAGGTGACTGGGAGATCCTGCAGAAATACTCGGGCTTTGAGTTCGACGATCTGTTCCCGAAATCGCAGGCGCGGAC
CGAGTATGAAAACATCCTCTATCTGGAGCGTCCGGGCTGTAACCTCTGCATGGGCAACCAGGAAAAGGCCGCCAAGGGTG
ACACCGTTCTGGCCACATCGACCCGCCTGTTCCAGGGCCGTGTGGTGGAAGATGCCGCCGAGAAGAAAGGCGAGAGCCTT
CTTGCCTCGACCCCGGTTGTGGTGCTCTCGGCCATTCTGGGTCGCACGCCAACGATCGAGGAATACAAGACCGCCGTGGA
GGGTATTGACCTCACCAAATTTGCCCCTCCGGTCGAAAAACCGGCAGGAACGCGCTCGGCGCATTTCTAA

Upstream 100 bases:

>100_bases
ATTGGTATACTGCCGCATACAGCAAGCTTGTCACGGCTCGCCAAGAGCGTTTGCGACCCCCGTTTCAGGCTTTTTCTTAC
ACAGGATCGTCGGGACCGTC

Downstream 100 bases:

>100_bases
ACACCTCTGCGCGGCGCAGACGGCTTTCTGGGGGCAATATGCTCACAGGTGACGTCCGCGGCGCGCAGTCTGGAAAGGTC
AGACCGACCGCCAGAATCCG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 929; Mature: 928

Protein sequence:

>929_residues
MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAE
VAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAE
FFTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV
GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLE
QKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVS
HEGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD
KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATG
EATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICIS
NDDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP
DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMK
AEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESL
LASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF

Sequences:

>Translated_929_residues
MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAE
VAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAE
FFTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV
GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLE
QKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVS
HEGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD
KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATG
EATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICIS
NDDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP
DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMK
AEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESL
LASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF
>Mature_928_residues
SLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAEV
AEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEF
FTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGVG
SSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLEQ
KYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSH
EGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWDK
KAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATGE
ATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISN
DDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADPD
VNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMKA
EGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLL
ASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=930, Percent_Identity=32.1505376344086, Blast_Score=329, Evalue=5e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 100779; Mature: 100647

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGT
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCC
TSAAGVKAAFLKEIILGEAEVAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQA
CCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHH
ADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEFFTKLPDVDDEIKVVTYIAA
HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
EGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV
CCCCCHHHCCCCCHHCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWV
CCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCHHHHH
KKVDSDGKAILNNDGNPVLEQKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKV
HHHCCCCCEEECCCCCCHHHHEECCCCCCEEEEECCCCHHHHCCCHHHHHHHCCCCCHHH
EFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSHEGQGLTAVEKIFNRNAVGS
HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCHHHHCCCCCCHHHHHHHHCCCCCCC
TPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD
CCCCEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHC
KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRM
CHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
SKGVAFGADSGTVALALATGEATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHG
HCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
DNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISNDDTLIGSLELAKSRIQIMI
CCCCCCEEEEEEECCHHHCCCEEEECCHHHHCCCEEEEECCCCCEEEHHHHHHCEEEEEE
DKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEHHHCCCCEECCC
DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKV
CCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHCCCEE
EFKAPLVVAAPTYNIIDEMKAEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGC
EEECCEEEECCCHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCHHHCCCHHCEEEEECCCC
NLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLLASTPVVVLSAILGRTPTIE
CEEECCCCCCCCCCEEEEECHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCHH
EYKTAVEGIDLTKFAPPVEKPAGTRSAHF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGT
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCC
TSAAGVKAAFLKEIILGEAEVAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQA
CCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHH
ADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEFFTKLPDVDDEIKVVTYIAA
HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
EGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV
CCCCCHHHCCCCCHHCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWV
CCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCHHHHH
KKVDSDGKAILNNDGNPVLEQKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKV
HHHCCCCCEEECCCCCCHHHHEECCCCCCEEEEECCCCHHHHCCCHHHHHHHCCCCCHHH
EFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSHEGQGLTAVEKIFNRNAVGS
HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCHHHHCCCCCCHHHHHHHHCCCCCCC
TPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD
CCCCEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHC
KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRM
CHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
SKGVAFGADSGTVALALATGEATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHG
HCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
DNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISNDDTLIGSLELAKSRIQIMI
CCCCCCEEEEEEECCHHHCCCEEEECCHHHHCCCEEEEECCCCCEEEHHHHHHCEEEEEE
DKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEHHHCCCCEECCC
DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKV
CCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHCCCEE
EFKAPLVVAAPTYNIIDEMKAEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGC
EEECCEEEECCCHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCHHHCCCHHCEEEEECCCC
NLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLLASTPVVVLSAILGRTPTIE
CEEECCCCCCCCCCEEEEECHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCHH
EYKTAVEGIDLTKFAPPVEKPAGTRSAHF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]