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The map label for this gene is lptD [H]

Identifier: 99080788

GI number: 99080788

Start: 1009832

End: 1012027

Strand: Reverse

Name: lptD [H]

Synonym: TM1040_0947

Alternate gene names: 99080788

Gene position: 1012027-1009832 (Counterclockwise)

Preceding gene: 99080789

Following gene: 99080787

Centisome position: 31.62

GC content: 60.97

Gene sequence:

>2196_bases
ATGCCGAAGACGGTTGAATTGAAGAAACGGCGGCGAGGCGCGCTGCTGCGGACGGTGGTACCCCTGTGGTTGGCGCTTGC
GGCAGCGCCCGCCCTGGCGCAAAACGCGACCTCTGCACAGGAAACCCCAGCCCCGGCGATGCTGGTGGCGGACCGGGTAT
TTGTATCCCCCGACCGCAAGCTGGTAGCCGAAGGAAATGTCGAGGCATTTCAGGGCGATATCCGCCTTCAGGCGCGGCGC
ATCTCTTATGATCGCCAAACCGGCATCCTTCAGATGGAGGGGCCGATCCGCATAGACCAGAGCGGCTCGATCACGGTACT
CGCCAATGCCGCCGAGCTGGACAGTGAATTGCGCAATGGCATCCTGCGTGGGGCGCGGATGGTCTTTGATCAGCAGGTGC
AGCTCGCAGCCCTGCAGATGACCCGCGTCGAGGGGCGCTATTCGCAGCTCTACAAGACCGCCGTGACCTCTTGCCATGTT
TGCGAAGATGGCAGCCCCCCGCTCTGGCAGATCCGCGCCGAGCGCATCACCCATGATCAGGCTGAGCGCCAGCTCTATCT
GGAGGGTGCGCAGCTGATGGTGAAAGATGTTCCGGTCTTTTACTTTCCCGCACTGCGCCTGCCAGACCCAACACTCGAGC
GGGCTGATGGCTTTCTGGTGCCCTCTCTCTCCAGTTCCTCGACGCTTGGCGTAGGGGTGAAGATCCCCTATTTCAAAACA
ATCGGCCCGCACAGAGACCTCACCATCACGCCCTACCTGTCCGAGAAGACCAGAACGCTCGACCTGCGCTACCGTCAGGC
CTTTCGCAACGGCCAGATCGAGATCACCGGCGCCTTCAGCCGCGATGACATTCAGCCTGATGATGGCCGGGGCTACCTCA
GCCTGCGCGGCGCCTTCGACATCCCGCGAGACTTCAAGCTGAGCTTTGATCTGAACACTGTCTCGGATGACGGATACTAC
GCTGACTATGACATCTCTGACACCGACCGCATTCGCTCCGAAATCTCGCTGATCCGGGTGCGGCGCGACCAGCTGATCGA
AGGCAAGATCTCCAACTACAAAACCCTGCGCGACGCCGAGAACCAGGACTTCATTCCCTCGACCATCGTGACCGGCACCT
TCGAGCAGCGCCTCTTTCCCAAGGCCGTTGGCGGCGAGCTCCGTCTGCGGCTCAACGCCTCGCAATTCAGACGCGAATCC
TCGCTTGATGCCACCCTCACAGATGCCAACGGGCGCGACATGAGCCGGGTTTCCGCCGATGCCACCTGGCTGCGCAGCTG
GATTTTGCCCTGGGGGATTGAATCGGTCTGGACCGCCGGGATCGGGATCGACAGTTTTGCGCTGTCAGATGATGCAGCCT
TTGACAATGATGCCACCCGCGTCACGCCCAAGGCGGCGCTGACCCTGCGCCGCCCGATGACACGCCAGACCGCATCAGGG
GCGGTTCAGGTGCTCGAGCCCATCGTGCAGCTTGGCTGGACCCATGTGAATGGCGACGACACCCCGAATGAGGCCAGCAA
TATCTCCGAGTTCGATCAGGGCAACCTGATGGCGCTCTCGCGGTTCCCCGAATCCGACGTGCGCGAGGATGGCGAGACGT
TTGTTTACGGCGTGAACTTTGCGCATTTCGACACCTCCGGCTGGTTTGCAACAGGTACCATTGCGCAGATCCACCGCGAT
CAAGCGCAATCGGGCTTTACCTCCTCCTCGGGGCTGGATGGCCGCAACTCCAACGTCTTGGTGGCGGGGCAGCTGGGCCT
GCGCAACGACCTGACGCTGACCGCCCGCACCCTCTTTGACGAGGAGTGGTCGGTGACCAAGGCAGAGTTTCGCGGCGATC
TGGAACGTGACCGGGTCAGTCTTGCAGGGAGCTATCTTTGGTTGCAGGCCGACGCGAGCGAGAACCGCACGGAAGAAACC
TCGGAACTCTGGTTCGATGGCACCTATGACTTGAACCAGACATGGCGCGCGGGCGCCAACATGCGCTACGACATTACCGA
TGGGCGCGCGACCCGTGCAGGTCTTGGCCTCACCTACAGCAATGAATGCGTGACACTCGACCTATCGCTCAGCCGTCGCT
ATACCTCGACGACAAGTGTTGAGCCATCAACGGATTTCGGATTTACACTGTCGCTCAACGGCTTTTCCGTCAAAAGCGGC
AACACAACAAGCAGGCGATCATGCAGCAAAACCTAA

Upstream 100 bases:

>100_bases
TTCGCGCGGATATTGGGCGAAAACGGGCAGATTCCGATCTTGCTGGCCGCCTGGGCGCCGCCGGTGGCCTCGATACTATT
GACGATGGGATTGCTCCTGC

Downstream 100 bases:

>100_bases
CCTTCCGCCAGATTTCGCTGGCCCTGATCAACTGGACCCGTCGCGCGAGCTACGCAGCAGGTCTGGCGCTTGCCATTGCC
GCGGCTGCCCCGGCGGATCT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MPKTVELKKRRRGALLRTVVPLWLALAAAPALAQNATSAQETPAPAMLVADRVFVSPDRKLVAEGNVEAFQGDIRLQARR
ISYDRQTGILQMEGPIRIDQSGSITVLANAAELDSELRNGILRGARMVFDQQVQLAALQMTRVEGRYSQLYKTAVTSCHV
CEDGSPPLWQIRAERITHDQAERQLYLEGAQLMVKDVPVFYFPALRLPDPTLERADGFLVPSLSSSSTLGVGVKIPYFKT
IGPHRDLTITPYLSEKTRTLDLRYRQAFRNGQIEITGAFSRDDIQPDDGRGYLSLRGAFDIPRDFKLSFDLNTVSDDGYY
ADYDISDTDRIRSEISLIRVRRDQLIEGKISNYKTLRDAENQDFIPSTIVTGTFEQRLFPKAVGGELRLRLNASQFRRES
SLDATLTDANGRDMSRVSADATWLRSWILPWGIESVWTAGIGIDSFALSDDAAFDNDATRVTPKAALTLRRPMTRQTASG
AVQVLEPIVQLGWTHVNGDDTPNEASNISEFDQGNLMALSRFPESDVREDGETFVYGVNFAHFDTSGWFATGTIAQIHRD
QAQSGFTSSSGLDGRNSNVLVAGQLGLRNDLTLTARTLFDEEWSVTKAEFRGDLERDRVSLAGSYLWLQADASENRTEET
SELWFDGTYDLNQTWRAGANMRYDITDGRATRAGLGLTYSNECVTLDLSLSRRYTSTTSVEPSTDFGFTLSLNGFSVKSG
NTTSRRSCSKT

Sequences:

>Translated_731_residues
MPKTVELKKRRRGALLRTVVPLWLALAAAPALAQNATSAQETPAPAMLVADRVFVSPDRKLVAEGNVEAFQGDIRLQARR
ISYDRQTGILQMEGPIRIDQSGSITVLANAAELDSELRNGILRGARMVFDQQVQLAALQMTRVEGRYSQLYKTAVTSCHV
CEDGSPPLWQIRAERITHDQAERQLYLEGAQLMVKDVPVFYFPALRLPDPTLERADGFLVPSLSSSSTLGVGVKIPYFKT
IGPHRDLTITPYLSEKTRTLDLRYRQAFRNGQIEITGAFSRDDIQPDDGRGYLSLRGAFDIPRDFKLSFDLNTVSDDGYY
ADYDISDTDRIRSEISLIRVRRDQLIEGKISNYKTLRDAENQDFIPSTIVTGTFEQRLFPKAVGGELRLRLNASQFRRES
SLDATLTDANGRDMSRVSADATWLRSWILPWGIESVWTAGIGIDSFALSDDAAFDNDATRVTPKAALTLRRPMTRQTASG
AVQVLEPIVQLGWTHVNGDDTPNEASNISEFDQGNLMALSRFPESDVREDGETFVYGVNFAHFDTSGWFATGTIAQIHRD
QAQSGFTSSSGLDGRNSNVLVAGQLGLRNDLTLTARTLFDEEWSVTKAEFRGDLERDRVSLAGSYLWLQADASENRTEET
SELWFDGTYDLNQTWRAGANMRYDITDGRATRAGLGLTYSNECVTLDLSLSRRYTSTTSVEPSTDFGFTLSLNGFSVKSG
NTTSRRSCSKT
>Mature_730_residues
PKTVELKKRRRGALLRTVVPLWLALAAAPALAQNATSAQETPAPAMLVADRVFVSPDRKLVAEGNVEAFQGDIRLQARRI
SYDRQTGILQMEGPIRIDQSGSITVLANAAELDSELRNGILRGARMVFDQQVQLAALQMTRVEGRYSQLYKTAVTSCHVC
EDGSPPLWQIRAERITHDQAERQLYLEGAQLMVKDVPVFYFPALRLPDPTLERADGFLVPSLSSSSTLGVGVKIPYFKTI
GPHRDLTITPYLSEKTRTLDLRYRQAFRNGQIEITGAFSRDDIQPDDGRGYLSLRGAFDIPRDFKLSFDLNTVSDDGYYA
DYDISDTDRIRSEISLIRVRRDQLIEGKISNYKTLRDAENQDFIPSTIVTGTFEQRLFPKAVGGELRLRLNASQFRRESS
LDATLTDANGRDMSRVSADATWLRSWILPWGIESVWTAGIGIDSFALSDDAAFDNDATRVTPKAALTLRRPMTRQTASGA
VQVLEPIVQLGWTHVNGDDTPNEASNISEFDQGNLMALSRFPESDVREDGETFVYGVNFAHFDTSGWFATGTIAQIHRDQ
AQSGFTSSSGLDGRNSNVLVAGQLGLRNDLTLTARTLFDEEWSVTKAEFRGDLERDRVSLAGSYLWLQADASENRTEETS
ELWFDGTYDLNQTWRAGANMRYDITDGRATRAGLGLTYSNECVTLDLSLSRRYTSTTSVEPSTDFGFTLSLNGFSVKSGN
TTSRRSCSKT

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=322, Percent_Identity=26.3975155279503, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 81328; Mature: 81197

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTVELKKRRRGALLRTVVPLWLALAAAPALAQNATSAQETPAPAMLVADRVFVSPDRK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECEEEECCCCE
LVAEGNVEAFQGDIRLQARRISYDRQTGILQMEGPIRIDQSGSITVLANAAELDSELRNG
EEECCCCEEEECCEEEEEEEECCCCCCCEEEECCCEEECCCCCEEEEECHHHHHHHHHHH
ILRGARMVFDQQVQLAALQMTRVEGRYSQLYKTAVTSCHVCEDGSPPLWQIRAERITHDQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCCCCEEHHHHHHHHHH
AERQLYLEGAQLMVKDVPVFYFPALRLPDPTLERADGFLVPSLSSSSTLGVGVKIPYFKT
HHHEEEECCCEEEEECCCEEEECCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCEEC
IGPHRDLTITPYLSEKTRTLDLRYRQAFRNGQIEITGAFSRDDIQPDDGRGYLSLRGAFD
CCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCC
IPRDFKLSFDLNTVSDDGYYADYDISDTDRIRSEISLIRVRRDQLIEGKISNYKTLRDAE
CCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
NQDFIPSTIVTGTFEQRLFPKAVGGELRLRLNASQFRRESSLDATLTDANGRDMSRVSAD
CCCCCCCCEEECCHHHHHCCHHCCCEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHHH
ATWLRSWILPWGIESVWTAGIGIDSFALSDDAAFDNDATRVTPKAALTLRRPMTRQTASG
HHHHHHHHCCCCCHHHHCCCCCCCCEECCCCCCCCCCCCEECCHHHEEECCCCCHHHHHH
AVQVLEPIVQLGWTHVNGDDTPNEASNISEFDQGNLMALSRFPESDVREDGETFVYGVNF
HHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEEEEEE
AHFDTSGWFATGTIAQIHRDQAQSGFTSSSGLDGRNSNVLVAGQLGLRNDLTLTARTLFD
EEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHCC
EEWSVTKAEFRGDLERDRVSLAGSYLWLQADASENRTEETSELWFDGTYDLNQTWRAGAN
CCCCEEHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCCCCCCHHHCCCC
MRYDITDGRATRAGLGLTYSNECVTLDLSLSRRYTSTTSVEPSTDFGFTLSLNGFSVKSG
EEEEECCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCEEEECC
NTTSRRSCSKT
CCCCCCCCCCC
>Mature Secondary Structure 
PKTVELKKRRRGALLRTVVPLWLALAAAPALAQNATSAQETPAPAMLVADRVFVSPDRK
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECEEEECCCCE
LVAEGNVEAFQGDIRLQARRISYDRQTGILQMEGPIRIDQSGSITVLANAAELDSELRNG
EEECCCCEEEECCEEEEEEEECCCCCCCEEEECCCEEECCCCCEEEEECHHHHHHHHHHH
ILRGARMVFDQQVQLAALQMTRVEGRYSQLYKTAVTSCHVCEDGSPPLWQIRAERITHDQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCCCCEEHHHHHHHHHH
AERQLYLEGAQLMVKDVPVFYFPALRLPDPTLERADGFLVPSLSSSSTLGVGVKIPYFKT
HHHEEEECCCEEEEECCCEEEECCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCEEC
IGPHRDLTITPYLSEKTRTLDLRYRQAFRNGQIEITGAFSRDDIQPDDGRGYLSLRGAFD
CCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCC
IPRDFKLSFDLNTVSDDGYYADYDISDTDRIRSEISLIRVRRDQLIEGKISNYKTLRDAE
CCCCEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
NQDFIPSTIVTGTFEQRLFPKAVGGELRLRLNASQFRRESSLDATLTDANGRDMSRVSAD
CCCCCCCCEEECCHHHHHCCHHCCCEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHHH
ATWLRSWILPWGIESVWTAGIGIDSFALSDDAAFDNDATRVTPKAALTLRRPMTRQTASG
HHHHHHHHCCCCCHHHHCCCCCCCCEECCCCCCCCCCCCEECCHHHEEECCCCCHHHHHH
AVQVLEPIVQLGWTHVNGDDTPNEASNISEFDQGNLMALSRFPESDVREDGETFVYGVNF
HHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEEEEEE
AHFDTSGWFATGTIAQIHRDQAQSGFTSSSGLDGRNSNVLVAGQLGLRNDLTLTARTLFD
EEECCCCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHCC
EEWSVTKAEFRGDLERDRVSLAGSYLWLQADASENRTEETSELWFDGTYDLNQTWRAGAN
CCCCEEHHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCCCCCCHHHCCCC
MRYDITDGRATRAGLGLTYSNECVTLDLSLSRRYTSTTSVEPSTDFGFTLSLNGFSVKSG
EEEEECCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCEEEECC
NTTSRRSCSKT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA