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The map label for this gene is hemK [H]

Identifier: 99080783

GI number: 99080783

Start: 1004793

End: 1005629

Strand: Direct

Name: hemK [H]

Synonym: TM1040_0942

Alternate gene names: 99080783

Gene position: 1004793-1005629 (Clockwise)

Preceding gene: 99080782

Following gene: 99080784

Centisome position: 31.39

GC content: 63.8

Gene sequence:

>837_bases
ATGAGCACCACCGCCGCACAAGCCATGGTGGCGGCAACGGCGCGCCTAAGAGCGGCGGGGGTAGAGGACCCCGCGCGCGA
TGCGCGGGTGCTTTTGGCGCATGCCGCGCGGATTGAGGCGGCGCGGGTCACATTGATCGCGCCCGAGGAACTCTCGCATG
AGGTTGCCGAACGCTATGACCAGCTGATTTCCCTGCGCGCGATCCGGGTACCCGTGTCGCATCTCGTGGGCGAGCGGGAT
TTCTATGGCCGCCGCTTCAAGGTCTCTGGCGATGTTCTGGACCCGCGTCCCGAAACCGAAACCCTGATCGAAGCGGCGCT
TGCAGAGCCGTTCGAGCGGGTGCTGGATCTTGGGGTGGGGTCGGGCTGCATTCTGGTGACGCTCTTGGCAGAGCAGCAGC
GCGCGCGCGGCCTTGGCGTGGACCTCAGTGAAGCCGCCTGTCTTCAGGCCAGCGCCAATGCGGTGTTGCACCGCGTCGAG
GCGCGTGCCGACATCCGCCAGTCGAACTGGTTTTCCGCTGTTGAAGGGCAGTTTGACCTGATTGTCTCCAACCCGCCTTA
TATTGCGCTTGAAGAAATGGATGGGCTCTCGGCAGAGGTGCGCGATCATGAACCGCAAATGGCGCTCACGGATGGGGCGG
ATGGCCTCAGCGCGTATCGGCAAATCTGTGCAGGGCTTGCGCCGCATCTCGCAGCAAATGGGCGCGTGATGGTAGAAATC
GGCCCCACGCAAGGTCCGGCTGTTGCGCAGATGATGCGCGAAACGGGGCTTCGCGATGTTAGCGTGCTGCCGGACCTGGA
TGGTCGGGATCGGGTCGTTTTTGGCCGAAATGCGTGA

Upstream 100 bases:

>100_bases
CGCTTTATAAGCTCGATCAGATCATGGGCGGCGACTTGGATGAAATCATTGACGCGCTGATATCCGAGCATCAGGCGAAG
CTCCTGGCGGAGATGGGGCA

Downstream 100 bases:

>100_bases
CGTGCCGCGTCAATAGCGCTCAATTTTGACAAAAAGAGCCAAAAAACTCCCCGTGCCCCCTTGTTTGTGACAAAGGAACG
TGGTTACTCAAGATGTCGCT

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSTTAAQAMVAATARLRAAGVEDPARDARVLLAHAARIEAARVTLIAPEELSHEVAERYDQLISLRAIRVPVSHLVGERD
FYGRRFKVSGDVLDPRPETETLIEAALAEPFERVLDLGVGSGCILVTLLAEQQRARGLGVDLSEAACLQASANAVLHRVE
ARADIRQSNWFSAVEGQFDLIVSNPPYIALEEMDGLSAEVRDHEPQMALTDGADGLSAYRQICAGLAPHLAANGRVMVEI
GPTQGPAVAQMMRETGLRDVSVLPDLDGRDRVVFGRNA

Sequences:

>Translated_278_residues
MSTTAAQAMVAATARLRAAGVEDPARDARVLLAHAARIEAARVTLIAPEELSHEVAERYDQLISLRAIRVPVSHLVGERD
FYGRRFKVSGDVLDPRPETETLIEAALAEPFERVLDLGVGSGCILVTLLAEQQRARGLGVDLSEAACLQASANAVLHRVE
ARADIRQSNWFSAVEGQFDLIVSNPPYIALEEMDGLSAEVRDHEPQMALTDGADGLSAYRQICAGLAPHLAANGRVMVEI
GPTQGPAVAQMMRETGLRDVSVLPDLDGRDRVVFGRNA
>Mature_277_residues
STTAAQAMVAATARLRAAGVEDPARDARVLLAHAARIEAARVTLIAPEELSHEVAERYDQLISLRAIRVPVSHLVGERDF
YGRRFKVSGDVLDPRPETETLIEAALAEPFERVLDLGVGSGCILVTLLAEQQRARGLGVDLSEAACLQASANAVLHRVEA
RADIRQSNWFSAVEGQFDLIVSNPPYIALEEMDGLSAEVRDHEPQMALTDGADGLSAYRQICAGLAPHLAANGRVMVEIG
PTQGPAVAQMMRETGLRDVSVLPDLDGRDRVVFGRNA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=300, Percent_Identity=29.6666666666667, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=35.6877323420074, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=34.1836734693878, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24582226, Length=279, Percent_Identity=27.9569892473118, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 29935; Mature: 29804

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTAAQAMVAATARLRAAGVEDPARDARVLLAHAARIEAARVTLIAPEELSHEVAERYD
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
QLISLRAIRVPVSHLVGERDFYGRRFKVSGDVLDPRPETETLIEAALAEPFERVLDLGVG
HHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SGCILVTLLAEQQRARGLGVDLSEAACLQASANAVLHRVEARADIRQSNWFSAVEGQFDL
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEE
IVSNPPYIALEEMDGLSAEVRDHEPQMALTDGADGLSAYRQICAGLAPHLAANGRVMVEI
EECCCCEEEEECCCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GPTQGPAVAQMMRETGLRDVSVLPDLDGRDRVVFGRNA
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCC
>Mature Secondary Structure 
STTAAQAMVAATARLRAAGVEDPARDARVLLAHAARIEAARVTLIAPEELSHEVAERYD
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
QLISLRAIRVPVSHLVGERDFYGRRFKVSGDVLDPRPETETLIEAALAEPFERVLDLGVG
HHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SGCILVTLLAEQQRARGLGVDLSEAACLQASANAVLHRVEARADIRQSNWFSAVEGQFDL
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEE
IVSNPPYIALEEMDGLSAEVRDHEPQMALTDGADGLSAYRQICAGLAPHLAANGRVMVEI
EECCCCEEEEECCCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
GPTQGPAVAQMMRETGLRDVSVLPDLDGRDRVVFGRNA
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]