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The map label for this gene is prfA

Identifier: 99080782

GI number: 99080782

Start: 1003744

End: 1004796

Strand: Direct

Name: prfA

Synonym: TM1040_0941

Alternate gene names: 99080782

Gene position: 1003744-1004796 (Clockwise)

Preceding gene: 99080780

Following gene: 99080783

Centisome position: 31.36

GC content: 62.87

Gene sequence:

>1053_bases
ATGATCCCCGAAGACCGGCTGCAACAGATTCTGCAACGCTACCAGTACCTCGAGGCCGCGATGGCGGATGGGGCAGGGGG
TGATATCGCCAAGCTGGCCAAGGAATATTCCGACCTGCGCCCGGTGGTGGAGCAGATCAGCGCCTATCAGCAGCTGTTGA
GCGATCTGGCCGAGGCCGAAGAAATGCTGGCCGACCCGGACATGAAGGCGCTCGCAGAGGAAGAGATCCCGACCCTGAAG
GCCGCACTGCCGCAGGCAGAGCATGCGCTGCAACTCGCGCTTTTGCCCAAGGATGAGGCCGACAGCCGAGCCGCCATTCT
CGAGATCCGACCCGGCACGGGCGGTGACGAGGCGGCGCTCTTTGCTGCGGATCTCTATCGCATGTACAGCCGCTATGCGG
AGACGCAGGGCTGGAAAGTGGATGTCATCGAAGAACAGGCCTCCGAGCTCGGCGGGCTCAAGGAACTGGTGGCGCGGATC
AATGGCGACGGGGTTTTCGCGCGGCTCAAGTATGAAAGCGGCGTGCATCGGGTCCAGCGGGTGCCCGTGACCGAGAGCGG
CGGGCGCATCCACACCTCTGCGGCGACAGTGGCGGTGCTGCCAGAGGCCGAGGATGTGGACATCAAGATCGACGCAAACG
ATCTGCGCATCGACACCATGCGGGCCTCGGGCGCGGGGGGGCAGCACGTCAACACCACCGACTCGGCGGTGCGGATCACC
CACCTGCCGACCGGGCTGGTGGTCACCAGTTCGGAAAAATCCCAGCACCGAAACCGCGAGATCGCAATGCAGGTGCTGAA
GACCCGGCTCTATGACCTTGAACGCCAGCGGATCGACACGGAACGCTCTGCGGATCGGGCTTCTCAGGTGGGGTCGGGAG
ATCGTTCGGAACGTATCCGGACCTATAACTTCCCGCAGGGGCGGATGTCGGACCACCGGATCAACCTCACGCTTTATAAG
CTCGATCAGATCATGGGCGGCGACTTGGATGAAATCATTGACGCGCTGATATCCGAGCATCAGGCGAAGCTCCTGGCGGA
GATGGGGCAATGA

Upstream 100 bases:

>100_bases
ATCGGACAAGACCACTGGCATGTTTCATGCCTCTAGCCTATCACAAATGCAGCCCCGCGCCAAAGCCTGACGCGCGCCCA
ACAGTGAGACACGCCAAGAC

Downstream 100 bases:

>100_bases
GCACCACCGCCGCACAAGCCATGGTGGCGGCAACGGCGCGCCTAAGAGCGGCGGGGGTAGAGGACCCCGCGCGCGATGCG
CGGGTGCTTTTGGCGCATGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIPEDRLQQILQRYQYLEAAMADGAGGDIAKLAKEYSDLRPVVEQISAYQQLLSDLAEAEEMLADPDMKALAEEEIPTLK
AALPQAEHALQLALLPKDEADSRAAILEIRPGTGGDEAALFAADLYRMYSRYAETQGWKVDVIEEQASELGGLKELVARI
NGDGVFARLKYESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEDVDIKIDANDLRIDTMRASGAGGQHVNTTDSAVRIT
HLPTGLVVTSSEKSQHRNREIAMQVLKTRLYDLERQRIDTERSADRASQVGSGDRSERIRTYNFPQGRMSDHRINLTLYK
LDQIMGGDLDEIIDALISEHQAKLLAEMGQ

Sequences:

>Translated_350_residues
MIPEDRLQQILQRYQYLEAAMADGAGGDIAKLAKEYSDLRPVVEQISAYQQLLSDLAEAEEMLADPDMKALAEEEIPTLK
AALPQAEHALQLALLPKDEADSRAAILEIRPGTGGDEAALFAADLYRMYSRYAETQGWKVDVIEEQASELGGLKELVARI
NGDGVFARLKYESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEDVDIKIDANDLRIDTMRASGAGGQHVNTTDSAVRIT
HLPTGLVVTSSEKSQHRNREIAMQVLKTRLYDLERQRIDTERSADRASQVGSGDRSERIRTYNFPQGRMSDHRINLTLYK
LDQIMGGDLDEIIDALISEHQAKLLAEMGQ
>Mature_350_residues
MIPEDRLQQILQRYQYLEAAMADGAGGDIAKLAKEYSDLRPVVEQISAYQQLLSDLAEAEEMLADPDMKALAEEEIPTLK
AALPQAEHALQLALLPKDEADSRAAILEIRPGTGGDEAALFAADLYRMYSRYAETQGWKVDVIEEQASELGGLKELVARI
NGDGVFARLKYESGVHRVQRVPVTESGGRIHTSAATVAVLPEAEDVDIKIDANDLRIDTMRASGAGGQHVNTTDSAVRIT
HLPTGLVVTSSEKSQHRNREIAMQVLKTRLYDLERQRIDTERSADRASQVGSGDRSERIRTYNFPQGRMSDHRINLTLYK
LDQIMGGDLDEIIDALISEHQAKLLAEMGQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=335, Percent_Identity=44.4776119402985, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=358, Percent_Identity=38.268156424581, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI166795305, Length=240, Percent_Identity=43.75, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1787462, Length=351, Percent_Identity=51.8518518518518, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI2367172, Length=308, Percent_Identity=39.9350649350649, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=295, Percent_Identity=35.9322033898305, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=332, Percent_Identity=41.8674698795181, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19921226, Length=357, Percent_Identity=41.7366946778711, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SILST (Q1GI42)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612936.1
- ProteinModelPortal:   Q1GI42
- SMR:   Q1GI42
- STRING:   Q1GI42
- GeneID:   4077335
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0941
- NMPDR:   fig|292414.1.peg.3021
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q1GI42
- ProtClustDB:   PRK00591
- BioCyc:   SSP292414:TM1040_0941-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38676; Mature: 38676

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEDRLQQILQRYQYLEAAMADGAGGDIAKLAKEYSDLRPVVEQISAYQQLLSDLAEAE
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMLADPDMKALAEEEIPTLKAALPQAEHALQLALLPKDEADSRAAILEIRPGTGGDEAAL
HHHCCCCHHHHHHHHCCHHHHHCCCHHHHEEEEECCCCCCCCCEEEEEEECCCCCCHHHH
FAADLYRMYSRYAETQGWKVDVIEEQASELGGLKELVARINGDGVFARLKYESGVHRVQR
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHCCCCEEEEEECHHHHHHHHC
VPVTESGGRIHTSAATVAVLPEAEDVDIKIDANDLRIDTMRASGAGGQHVNTTDSAVRIT
CCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEE
HLPTGLVVTSSEKSQHRNREIAMQVLKTRLYDLERQRIDTERSADRASQVGSGDRSERIR
ECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCE
TYNFPQGRMSDHRINLTLYKLDQIMGGDLDEIIDALISEHQAKLLAEMGQ
ECCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPEDRLQQILQRYQYLEAAMADGAGGDIAKLAKEYSDLRPVVEQISAYQQLLSDLAEAE
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMLADPDMKALAEEEIPTLKAALPQAEHALQLALLPKDEADSRAAILEIRPGTGGDEAAL
HHHCCCCHHHHHHHHCCHHHHHCCCHHHHEEEEECCCCCCCCCEEEEEEECCCCCCHHHH
FAADLYRMYSRYAETQGWKVDVIEEQASELGGLKELVARINGDGVFARLKYESGVHRVQR
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHCCCCEEEEEECHHHHHHHHC
VPVTESGGRIHTSAATVAVLPEAEDVDIKIDANDLRIDTMRASGAGGQHVNTTDSAVRIT
CCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEE
HLPTGLVVTSSEKSQHRNREIAMQVLKTRLYDLERQRIDTERSADRASQVGSGDRSERIR
ECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCE
TYNFPQGRMSDHRINLTLYKLDQIMGGDLDEIIDALISEHQAKLLAEMGQ
ECCCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA