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The map label for this gene is nreB [H]

Identifier: 99080718

GI number: 99080718

Start: 935016

End: 936440

Strand: Reverse

Name: nreB [H]

Synonym: TM1040_0877

Alternate gene names: 99080718

Gene position: 936440-935016 (Counterclockwise)

Preceding gene: 99080722

Following gene: 99080717

Centisome position: 29.26

GC content: 62.04

Gene sequence:

>1425_bases
ATGATCCGGCGCATGTTCCGCCCGAATTACGCCCAAAAACTGTCCCTGCTGGCCACTTTGCCCTTAATCGTGGCCTGCCT
GTGCATCGCGACCCTGGTGGCCTATCAATCCCGCGCCATGGCTGAACGCGAAATTCAGGTGCTGGAGCGTCAGTTGATCG
AGGCCAAGAAGGCCGAACTGCGCAACTACGTCACCCAGGCCCGCAACGGCTTTATGCATATTTATGGCCCCGCCGCCCCG
GACGACACCGAGGCCAAGCGCAAGGTCACCCAGATCCTGAGCGCCATGATCTACGGCCAGGACGGGTTCTTTTTTGTCTA
TGATTACGATGGCACCAACCTGGTCAGCCCGCGTCAGACCGAGTTTATCAACCGCAACTGGGCGGGACTGACGGATGCCA
ATGGCGTGCCGGTGGTCAACACTTTCATCGACATCGCGCGTCAGGGTGCCGGCTGGCACAGTTTCATGTGGGAAAAGCCC
TCCACCGGTGAAGAGGCGCAGATGATCGCCTATGTGGTCGGGCTTCAAGACTGGCAATGGGCAGTGGGCACCGGGGTTTT
TATCGACGATGTGATCGCCTCGATTGCCAGCTCGCGTCAGGAGGTCGAAAACCGGGTGCGTCGCACATTCCTCTATATAG
GTGCGATTACCCTTGTGGCGTTGATCGCGGTGTTTGGCTCTGGGATGCTCCTCAACATCCGCGAGCGGCGTCTGGCCGAC
GCCAAGCTCAAGGAGCTCACGCAGCGGGTCTTTGATGCCCAGGAAGAAGAGCGCGGGCGGGTGGCGCGCGAGCTCCATGA
TTCGATCAGCCAGTTGCTGGTCGGGGTACGCTATGCGCTGGAAACCGTCAGGCGGCGCATCGGGCGGGGCGAGTTCGAGA
CCACCGCCGCGCCGCTCGACAAGGGCGTCGAAGGTCTGGCGACCGCCATCACCGAAGTACGCCGCATCAGCCGTGATCTG
CGCCCCGGCGTGCTGGATGATCTCGGGCTTGGCCCCGCGCTCAAGGCGCTGACGGATGACTTTGCAGCCCGCACCGGGAT
CGAAACGGATTTCACTACTGTGGTCTTTCGCAACCGCCTCGATCAGGAGGCCAAGATTGCGCTTTACCGGATTGCCCAGG
AGGCGCTCACCAATATCGAACGCCATTCGGGTGCCACGCGGGTGACGATGGATCTGCGCGGCCATGAAAACGGCGCCACC
ATGCGCATCTCAGACAATGGCTCTGGCCTGCCCACCGAAGAGGAGCGCAGCCGCTCCGGGATTGGACTGCGCAACATGCA
AGAGCGTATGGAACAGCTTGACGGCACCCTGCGCATTCTGTCATTTCGCGCAGGCCGCGACCGTGGCACAGTCATCATCG
CCAGCCTGCCCCTGAGCCACCTGCTCCCGCCGGGCGACGAGCTTGCAGCGCATCGCCTGCCCTGA

Upstream 100 bases:

>100_bases
CGCCCGGCCGTTTCAGGGCTTTGATTCTCGACCTCAGTGCCGTAACGATTCGAAACGGCCCCGTCGCCGCTCCCGATGGC
CTTTTCAGGAACCAGAAACG

Downstream 100 bases:

>100_bases
CAGCCAGCAGGACACAGCAGATGCCAGGGAGGGCCTCTGATGAAGAACGCCAGACCGACAACATCGGGAGACAAGAGATG
AATGGCCCAATCCGCGTTCT

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIRRMFRPNYAQKLSLLATLPLIVACLCIATLVAYQSRAMAEREIQVLERQLIEAKKAELRNYVTQARNGFMHIYGPAAP
DDTEAKRKVTQILSAMIYGQDGFFFVYDYDGTNLVSPRQTEFINRNWAGLTDANGVPVVNTFIDIARQGAGWHSFMWEKP
STGEEAQMIAYVVGLQDWQWAVGTGVFIDDVIASIASSRQEVENRVRRTFLYIGAITLVALIAVFGSGMLLNIRERRLAD
AKLKELTQRVFDAQEEERGRVARELHDSISQLLVGVRYALETVRRRIGRGEFETTAAPLDKGVEGLATAITEVRRISRDL
RPGVLDDLGLGPALKALTDDFAARTGIETDFTTVVFRNRLDQEAKIALYRIAQEALTNIERHSGATRVTMDLRGHENGAT
MRISDNGSGLPTEEERSRSGIGLRNMQERMEQLDGTLRILSFRAGRDRGTVIIASLPLSHLLPPGDELAAHRLP

Sequences:

>Translated_474_residues
MIRRMFRPNYAQKLSLLATLPLIVACLCIATLVAYQSRAMAEREIQVLERQLIEAKKAELRNYVTQARNGFMHIYGPAAP
DDTEAKRKVTQILSAMIYGQDGFFFVYDYDGTNLVSPRQTEFINRNWAGLTDANGVPVVNTFIDIARQGAGWHSFMWEKP
STGEEAQMIAYVVGLQDWQWAVGTGVFIDDVIASIASSRQEVENRVRRTFLYIGAITLVALIAVFGSGMLLNIRERRLAD
AKLKELTQRVFDAQEEERGRVARELHDSISQLLVGVRYALETVRRRIGRGEFETTAAPLDKGVEGLATAITEVRRISRDL
RPGVLDDLGLGPALKALTDDFAARTGIETDFTTVVFRNRLDQEAKIALYRIAQEALTNIERHSGATRVTMDLRGHENGAT
MRISDNGSGLPTEEERSRSGIGLRNMQERMEQLDGTLRILSFRAGRDRGTVIIASLPLSHLLPPGDELAAHRLP
>Mature_474_residues
MIRRMFRPNYAQKLSLLATLPLIVACLCIATLVAYQSRAMAEREIQVLERQLIEAKKAELRNYVTQARNGFMHIYGPAAP
DDTEAKRKVTQILSAMIYGQDGFFFVYDYDGTNLVSPRQTEFINRNWAGLTDANGVPVVNTFIDIARQGAGWHSFMWEKP
STGEEAQMIAYVVGLQDWQWAVGTGVFIDDVIASIASSRQEVENRVRRTFLYIGAITLVALIAVFGSGMLLNIRERRLAD
AKLKELTQRVFDAQEEERGRVARELHDSISQLLVGVRYALETVRRRIGRGEFETTAAPLDKGVEGLATAITEVRRISRDL
RPGVLDDLGLGPALKALTDDFAARTGIETDFTTVVFRNRLDQEAKIALYRIAQEALTNIERHSGATRVTMDLRGHENGAT
MRISDNGSGLPTEEERSRSGIGLRNMQERMEQLDGTLRILSFRAGRDRGTVIIASLPLSHLLPPGDELAAHRLP

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4564

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=219, Percent_Identity=31.9634703196347, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787474, Length=248, Percent_Identity=27.4193548387097, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788812, Length=204, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52959; Mature: 52959

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRMFRPNYAQKLSLLATLPLIVACLCIATLVAYQSRAMAEREIQVLERQLIEAKKAEL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNYVTQARNGFMHIYGPAAPDDTEAKRKVTQILSAMIYGQDGFFFVYDYDGTNLVSPRQT
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
EFINRNWAGLTDANGVPVVNTFIDIARQGAGWHSFMWEKPSTGEEAQMIAYVVGLQDWQW
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCH
AVGTGVFIDDVIASIASSRQEVENRVRRTFLYIGAITLVALIAVFGSGMLLNIRERRLAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
AKLKELTQRVFDAQEEERGRVARELHDSISQLLVGVRYALETVRRRIGRGEFETTAAPLD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KGVEGLATAITEVRRISRDLRPGVLDDLGLGPALKALTDDFAARTGIETDFTTVVFRNRL
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DQEAKIALYRIAQEALTNIERHSGATRVTMDLRGHENGATMRISDNGSGLPTEEERSRSG
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHCC
IGLRNMQERMEQLDGTLRILSFRAGRDRGTVIIASLPLSHLLPPGDELAAHRLP
CCHHHHHHHHHHHCCHHEEHEEECCCCCCEEEEEECCHHHCCCCCHHHHCCCCC
>Mature Secondary Structure
MIRRMFRPNYAQKLSLLATLPLIVACLCIATLVAYQSRAMAEREIQVLERQLIEAKKAEL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNYVTQARNGFMHIYGPAAPDDTEAKRKVTQILSAMIYGQDGFFFVYDYDGTNLVSPRQT
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
EFINRNWAGLTDANGVPVVNTFIDIARQGAGWHSFMWEKPSTGEEAQMIAYVVGLQDWQW
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCH
AVGTGVFIDDVIASIASSRQEVENRVRRTFLYIGAITLVALIAVFGSGMLLNIRERRLAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
AKLKELTQRVFDAQEEERGRVARELHDSISQLLVGVRYALETVRRRIGRGEFETTAAPLD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KGVEGLATAITEVRRISRDLRPGVLDDLGLGPALKALTDDFAARTGIETDFTTVVFRNRL
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DQEAKIALYRIAQEALTNIERHSGATRVTMDLRGHENGATMRISDNGSGLPTEEERSRSG
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHCC
IGLRNMQERMEQLDGTLRILSFRAGRDRGTVIIASLPLSHLLPPGDELAAHRLP
CCHHHHHHHHHHHCCHHEEHEEECCCCCCEEEEEECCHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]