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The map label for this gene is vraR [H]

Identifier: 99080717

GI number: 99080717

Start: 934294

End: 934995

Strand: Reverse

Name: vraR [H]

Synonym: TM1040_0876

Alternate gene names: 99080717

Gene position: 934995-934294 (Counterclockwise)

Preceding gene: 99080718

Following gene: 99080716

Centisome position: 29.21

GC content: 60.97

Gene sequence:

>702_bases
ATGCCAGGGAGGGCCTCTGATGAAGAACGCCAGACCGACAACATCGGGAGACAAGAGATGAATGGCCCAATCCGCGTTCT
GATCGTCGATGACCACCCGATGGTCGCCGAAGGGATCCAGTCGATCCTCGAAAGCTACGGCGACATCGAAGTGGTCGGGG
CCGTTTCAAGCGGGCGTGAGGCCATTGCCCGCGCCGAGGCTCTCGTGCCGGATGTCATCCTGATGGATCTCAACATGCCT
GATGTGGGCGGCCTTAGCGCGACCGAGATCATTCAGGAACGCTGCCCTGAAATCCGGGTGCTGATCCTGTCGATGCACGA
CAGCCCGGAATATATCACCACCGCCCTCAGCCACGGTGCGGTTGGCTATGTTCTGAAGGATGTCCAGCCCGAAGAAATCA
AGACCGCAATCGACGCGGTGATGCGCGGCGAACAATACCTGTGCACCGGCGCCGCCGGGTCGCTCTCGCCGCAATCCCAG
ACCAGCCGTGAAGCGCTCACAGGGCGCGAGCAGACGATCCTCTTGGAACTGGCGCAGGGCAAATCCAACAAGGAAGTGGC
GCAAGCGCTCGATATTTCCGTGCGCACGGTGGAAACCCACCGCAAGAACATCAAGCGCAAACTTGGGATCTCTTCGACCG
CGGGGCTCACCCGCTATGCGCTGGAACACGGTGTCCTGCAAGGCACCGGCCGCGGTATCTGA

Upstream 100 bases:

>100_bases
GCACAGTCATCATCGCCAGCCTGCCCCTGAGCCACCTGCTCCCGCCGGGCGACGAGCTTGCAGCGCATCGCCTGCCCTGA
CAGCCAGCAGGACACAGCAG

Downstream 100 bases:

>100_bases
TCCGTAGGGCCCAGCGCACCAGAGGCTCTGAAACCTTCGCATTAGCCACGCCTCTCCGCCCCCCCATTGCGCTGCATGCC
CGCCCCGCCCGATAGGGCTC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPGRASDEERQTDNIGRQEMNGPIRVLIVDDHPMVAEGIQSILESYGDIEVVGAVSSGREAIARAEALVPDVILMDLNMP
DVGGLSATEIIQERCPEIRVLILSMHDSPEYITTALSHGAVGYVLKDVQPEEIKTAIDAVMRGEQYLCTGAAGSLSPQSQ
TSREALTGREQTILLELAQGKSNKEVAQALDISVRTVETHRKNIKRKLGISSTAGLTRYALEHGVLQGTGRGI

Sequences:

>Translated_233_residues
MPGRASDEERQTDNIGRQEMNGPIRVLIVDDHPMVAEGIQSILESYGDIEVVGAVSSGREAIARAEALVPDVILMDLNMP
DVGGLSATEIIQERCPEIRVLILSMHDSPEYITTALSHGAVGYVLKDVQPEEIKTAIDAVMRGEQYLCTGAAGSLSPQSQ
TSREALTGREQTILLELAQGKSNKEVAQALDISVRTVETHRKNIKRKLGISSTAGLTRYALEHGVLQGTGRGI
>Mature_232_residues
PGRASDEERQTDNIGRQEMNGPIRVLIVDDHPMVAEGIQSILESYGDIEVVGAVSSGREAIARAEALVPDVILMDLNMPD
VGGLSATEIIQERCPEIRVLILSMHDSPEYITTALSHGAVGYVLKDVQPEEIKTAIDAVMRGEQYLCTGAAGSLSPQSQT
SREALTGREQTILLELAQGKSNKEVAQALDISVRTVETHRKNIKRKLGISSTAGLTRYALEHGVLQGTGRGI

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=32.6923076923077, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=35.9223300970874, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=35.678391959799, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787473, Length=185, Percent_Identity=38.9189189189189, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=29.2079207920792, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788712, Length=200, Percent_Identity=28.5, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788546, Length=205, Percent_Identity=27.8048780487805, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082052, Length=156, Percent_Identity=31.4102564102564, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25112; Mature: 24981

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGRASDEERQTDNIGRQEMNGPIRVLIVDDHPMVAEGIQSILESYGDIEVVGAVSSGRE
CCCCCCCHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHH
AIARAEALVPDVILMDLNMPDVGGLSATEIIQERCPEIRVLILSMHDSPEYITTALSHGA
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCC
VGYVLKDVQPEEIKTAIDAVMRGEQYLCTGAAGSLSPQSQTSREALTGREQTILLELAQG
HHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCC
KSNKEVAQALDISVRTVETHRKNIKRKLGISSTAGLTRYALEHGVLQGTGRGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PGRASDEERQTDNIGRQEMNGPIRVLIVDDHPMVAEGIQSILESYGDIEVVGAVSSGRE
CCCCCCHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHH
AIARAEALVPDVILMDLNMPDVGGLSATEIIQERCPEIRVLILSMHDSPEYITTALSHGA
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCC
VGYVLKDVQPEEIKTAIDAVMRGEQYLCTGAAGSLSPQSQTSREALTGREQTILLELAQG
HHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCC
KSNKEVAQALDISVRTVETHRKNIKRKLGISSTAGLTRYALEHGVLQGTGRGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]