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The map label for this gene is ilvI [H]

Identifier: 99080716

GI number: 99080716

Start: 931943

End: 933694

Strand: Reverse

Name: ilvI [H]

Synonym: TM1040_0875

Alternate gene names: 99080716

Gene position: 933694-931943 (Counterclockwise)

Preceding gene: 99080717

Following gene: 99080715

Centisome position: 29.17

GC content: 60.56

Gene sequence:

>1752_bases
ATGACACGTGAAATGACCGGCGCAAAAATGGTTGTCCAAGCCCTCAAGGAGCAGGGCGTGGACACGGTATTCGGATATCC
CGGAGGCGCTGTCCTACCCATTTACGATGAAATCTTTCAGCAAAATGACATTCGCCATATTCTGGTCCGTCACGAGCAGG
GCGCAGTGCATGCCGCCGAAGGGTATGCGCGCTCGACCGGCAAACCCGGCGTGGTGCTTGTGACCTCTGGCCCCGGTGCC
ACCAACGCGGTGACCGGCCTCACGGATGCGCTGCTGGACTCGATCCCGCTGGTGGTTCTCACCGGGCAGGTCCCGACCTT
CATGATCGGCTCTGACGCCTTTCAGGAGGCCGACACCGTCGGCATCACCCGCCCCTGCACCAAGCACAACTGGCTGGTGA
AGGACACCGATAAACTCGCCTCCACCATCCACGAAGGGTTCCATGTCGCAACCTCTGGCCGCCCTGGCCCGGTGCTGATC
GACATTCCCAAGGACGTGCAGTTTGCCACCGGCACCTATGAGCCCAAGAAACCCTCGGCTTCGCATTACCAGCCGGTCGT
CAAGGGCGACATGGAAGAAATCACCGAGCTGGTCGCCGCGATGGAGACCGCCAAACGCCCGGTGTTTTATACCGGCGGCG
GCGTGATCAACTCGGGCCCGGCCGCAAGCCAGCTTCTGCGCGAACTGGTGGAGGCCACCGGCTTTCCGATCACCTCGACC
CTGATGGGTCTCGGTGCCTATCCCGCGTCGGGCAAGCAGTGGCTTGGGATGCTTGGCATGCATGGTCTCTACGAGGCCAA
TATGGCGATGCATGACTGCGACCTGATGATCAACATCGGCGCGCGCTTTGATGACCGGATCACCGGTCGCATCGACGCCT
TCAGCCCGAAATCCATCAAGGCCCATATCGACATCGACCCCTCCTCGATCAACAAGGTGATCAAGGCGGACATCCCGATT
GTCGGGGACGTTGGCCATGTGCTTGAGGACATTCTCAAGGTCTGGAAGAGCCGCGGGCGCAAGACCAACGCCGAAGCACT
GGCAAAATGGCAGGGCCAGATCGACGAATGGCGCGCGGTGAAATGCCTGACCTATGAGATGTCCGAAACCACCATCAAGC
CGCAATATGCGCTTGAGCGTCTCGAGGCGCTGACCAAGGGTCGGGATCGCTATATCACCACCGAAGTGGGCCAGCACCAG
ATGTGGGCAGCACAGTTCCTGGGCTTTGAAGACCCCAACCGCTGGATGACCTCCGGGGGGCTTGGCACCATGGGCTATGG
TACGCCTGCCTCTATCGGCGCGCAGATCGCGCATCCCGATGCGCTGGTGATCAACGTCGCGGGCGAGGCCTCTTGGCTGA
TGAACATGCAGGAAATGGGCACTGCGACCCAGTACCGCCTGCCAGTGAAACAGTTCATCCTCAACAACGAACGCCTTGGC
ATGGTGCGCCAGTGGCAGGAGCTCTTGCATGGTGAGCGCTACTCGCACAGCTGGTCCGAAGCGCTGCCCGATTTTGTCAA
ACTCGCCGAAGCCTTTGGCGCCAAGGGCATCATCTGCTCGGACCCCAAGGATCTGGATGACGCGATCATGGAGATGATCG
AATATGACGGGCCGGTGATCTTTGACTGTCTGGTGGAAAAGCACGAGAACTGCTTCCCGATGATCCCCTCGGGCAAGGCT
CACAACGAGATGCTGTTGGGCGCGGCTGAAACGCAGGGCGTGATCCAGTCCGGCGGCGCGGTTCTGGTCTGA

Upstream 100 bases:

>100_bases
ATGCGCCTCCGATCCTCAAAATCGGGGGCTTTTTTATGCGATACACACTGACGTTAGACTGACGCAAAGAAAGACGATGT
CGTGAACTGGAGCAAAGCAG

Downstream 100 bases:

>100_bases
TCATCTGAGGTTTGATTGCAAGGCGTATCGCGCCTTGCCCCGATATTTCGAGGAAAGGGACTGACATGTCTGCCCTACAC
ATCAAAAAAGGTGCTACCAA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MTREMTGAKMVVQALKEQGVDTVFGYPGGAVLPIYDEIFQQNDIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSGPGA
TNAVTGLTDALLDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKDTDKLASTIHEGFHVATSGRPGPVLI
DIPKDVQFATGTYEPKKPSASHYQPVVKGDMEEITELVAAMETAKRPVFYTGGGVINSGPAASQLLRELVEATGFPITST
LMGLGAYPASGKQWLGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRIDAFSPKSIKAHIDIDPSSINKVIKADIPI
VGDVGHVLEDILKVWKSRGRKTNAEALAKWQGQIDEWRAVKCLTYEMSETTIKPQYALERLEALTKGRDRYITTEVGQHQ
MWAAQFLGFEDPNRWMTSGGLGTMGYGTPASIGAQIAHPDALVINVAGEASWLMNMQEMGTATQYRLPVKQFILNNERLG
MVRQWQELLHGERYSHSWSEALPDFVKLAEAFGAKGIICSDPKDLDDAIMEMIEYDGPVIFDCLVEKHENCFPMIPSGKA
HNEMLLGAAETQGVIQSGGAVLV

Sequences:

>Translated_583_residues
MTREMTGAKMVVQALKEQGVDTVFGYPGGAVLPIYDEIFQQNDIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSGPGA
TNAVTGLTDALLDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKDTDKLASTIHEGFHVATSGRPGPVLI
DIPKDVQFATGTYEPKKPSASHYQPVVKGDMEEITELVAAMETAKRPVFYTGGGVINSGPAASQLLRELVEATGFPITST
LMGLGAYPASGKQWLGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRIDAFSPKSIKAHIDIDPSSINKVIKADIPI
VGDVGHVLEDILKVWKSRGRKTNAEALAKWQGQIDEWRAVKCLTYEMSETTIKPQYALERLEALTKGRDRYITTEVGQHQ
MWAAQFLGFEDPNRWMTSGGLGTMGYGTPASIGAQIAHPDALVINVAGEASWLMNMQEMGTATQYRLPVKQFILNNERLG
MVRQWQELLHGERYSHSWSEALPDFVKLAEAFGAKGIICSDPKDLDDAIMEMIEYDGPVIFDCLVEKHENCFPMIPSGKA
HNEMLLGAAETQGVIQSGGAVLV
>Mature_582_residues
TREMTGAKMVVQALKEQGVDTVFGYPGGAVLPIYDEIFQQNDIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSGPGAT
NAVTGLTDALLDSIPLVVLTGQVPTFMIGSDAFQEADTVGITRPCTKHNWLVKDTDKLASTIHEGFHVATSGRPGPVLID
IPKDVQFATGTYEPKKPSASHYQPVVKGDMEEITELVAAMETAKRPVFYTGGGVINSGPAASQLLRELVEATGFPITSTL
MGLGAYPASGKQWLGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRIDAFSPKSIKAHIDIDPSSINKVIKADIPIV
GDVGHVLEDILKVWKSRGRKTNAEALAKWQGQIDEWRAVKCLTYEMSETTIKPQYALERLEALTKGRDRYITTEVGQHQM
WAAQFLGFEDPNRWMTSGGLGTMGYGTPASIGAQIAHPDALVINVAGEASWLMNMQEMGTATQYRLPVKQFILNNERLGM
VRQWQELLHGERYSHSWSEALPDFVKLAEAFGAKGIICSDPKDLDDAIMEMIEYDGPVIFDCLVEKHENCFPMIPSGKAH
NEMLLGAAETQGVIQSGGAVLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.3816254416961, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=24.7058823529412, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=48.951048951049, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.1985815602837, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=31.5789473684211, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=27.0609318996416, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788716, Length=570, Percent_Identity=24.5614035087719, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=24.7330960854093, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=24.7330960854093, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=518, Percent_Identity=26.6409266409266, Blast_Score=132, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=45.2991452991453, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320816, Length=500, Percent_Identity=23.6, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=588, Percent_Identity=22.108843537415, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=25.7894736842105, Blast_Score=153, Evalue=4e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63495; Mature: 63364

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREMTGAKMVVQALKEQGVDTVFGYPGGAVLPIYDEIFQQNDIRHILVRHEQGAVHAAE
CCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHCCCCCEEEEEECCCCCEEHHC
GYARSTGKPGVVLVTSGPGATNAVTGLTDALLDSIPLVVLTGQVPTFMIGSDAFQEADTV
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHCCC
GITRPCTKHNWLVKDTDKLASTIHEGFHVATSGRPGPVLIDIPKDVQFATGTYEPKKPSA
CCCCCCCCCCCEEECHHHHHHHHHHCEEEEECCCCCCEEEECCCCCEECCCCCCCCCCCC
SHYQPVVKGDMEEITELVAAMETAKRPVFYTGGGVINSGPAASQLLRELVEATGFPITST
CCCCCHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPASGKQWLGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRIDAFSPKSIK
HHHCCCCCCCCHHHHHHHCCCCHHHCCCEEECCEEEEEECCCCCCCCCCEEECCCCCCEE
AHIDIDPSSINKVIKADIPIVGDVGHVLEDILKVWKSRGRKTNAEALAKWQGQIDEWRAV
EEEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEE
KCLTYEMSETTIKPQYALERLEALTKGRDRYITTEVGQHQMWAAQFLGFEDPNRWMTSGG
EEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEECCC
LGTMGYGTPASIGAQIAHPDALVINVAGEASWLMNMQEMGTATQYRLPVKQFILNNERLG
CCCCCCCCCHHCCCEECCCCEEEEEECCCHHHHHHHHHHCCCCEECCCHHHHHHCCCCCH
MVRQWQELLHGERYSHSWSEALPDFVKLAEAFGAKGIICSDPKDLDDAIMEMIEYDGPVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEE
FDCLVEKHENCFPMIPSGKAHNEMLLGAAETQGVIQSGGAVLV
EHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCEEEC
>Mature Secondary Structure 
TREMTGAKMVVQALKEQGVDTVFGYPGGAVLPIYDEIFQQNDIRHILVRHEQGAVHAAE
CCCCHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHCCCCCEEEEEECCCCCEEHHC
GYARSTGKPGVVLVTSGPGATNAVTGLTDALLDSIPLVVLTGQVPTFMIGSDAFQEADTV
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHCCC
GITRPCTKHNWLVKDTDKLASTIHEGFHVATSGRPGPVLIDIPKDVQFATGTYEPKKPSA
CCCCCCCCCCCEEECHHHHHHHHHHCEEEEECCCCCCEEEECCCCCEECCCCCCCCCCCC
SHYQPVVKGDMEEITELVAAMETAKRPVFYTGGGVINSGPAASQLLRELVEATGFPITST
CCCCCHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHH
LMGLGAYPASGKQWLGMLGMHGLYEANMAMHDCDLMINIGARFDDRITGRIDAFSPKSIK
HHHCCCCCCCCHHHHHHHCCCCHHHCCCEEECCEEEEEECCCCCCCCCCEEECCCCCCEE
AHIDIDPSSINKVIKADIPIVGDVGHVLEDILKVWKSRGRKTNAEALAKWQGQIDEWRAV
EEEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEE
KCLTYEMSETTIKPQYALERLEALTKGRDRYITTEVGQHQMWAAQFLGFEDPNRWMTSGG
EEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEECCC
LGTMGYGTPASIGAQIAHPDALVINVAGEASWLMNMQEMGTATQYRLPVKQFILNNERLG
CCCCCCCCCHHCCCEECCCCEEEEEECCCHHHHHHHHHHCCCCEECCCHHHHHHCCCCCH
MVRQWQELLHGERYSHSWSEALPDFVKLAEAFGAKGIICSDPKDLDDAIMEMIEYDGPVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEE
FDCLVEKHENCFPMIPSGKAHNEMLLGAAETQGVIQSGGAVLV
EHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]