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The map label for this gene is prfB

Identifier: 99080709

GI number: 99080709

Start: 924764

End: 925888

Strand: Direct

Name: prfB

Synonym: TM1040_0868

Alternate gene names: 99080709

Gene position: 924764-925888 (Clockwise)

Preceding gene: 99080708

Following gene: 99080711

Centisome position: 28.89

GC content: 61.16

Gene sequence:

>1125_bases
ATGCGCGCCGAGATCCAGACCATCGTGGCCGATATCGAAAAATCGCTCGACCTGCTTGCCCAGCGGATGAACTGGGAGAC
GGCGGAATTCCGGCTTGAGGAATTCAACGCGCGGGTCGAAGATCCCAACCTCTGGGATGACCCTGCAGCGGCGCAAAAGC
TGATGCGGGACCGTCAGTCCCTCGTGGACGCAATGGACACCTACAAGAGCATCCGCCAGGACCTTCAGGACAGCGTTGAG
CTGATCGAGCTTGGCGAGATGGAAGAGGATGCCGAAGTCGTCAAAGACGCCGAAGACACCCTTAAGGCACTGAAGGAAAA
AGCCGCCCAGAAAGAGCTTGAAGCGCTTCTGGACGGTGAAACCGACCCCAATGACGCCTTCCTCGAGATCAAGGCGGGCG
CGGGCGGCACCGAGGCCTGCGACTGGGCGTCGATGTTGGCGCGCATGTATGTGCGCTGGGCCGAGAGCCGAGGCTTCAGC
GTCGAGCTGCAGGAAGAGCAGGCGGGCGCCGAGGCCGGCATTAAATCCGCCACCTATCAGATCAAGGGTCACAACGCCTA
TGGCTGGCTGAAGTCCGAGAGCGGCACCCACCGTCTTGTGCGGATTTCGCCCTTTGGCAAAGGCACGCGCGAGACGTCAT
TTGCCGCCGTGGGCGCCTATCCGGTGATCGATGACAACATCGAAATTGAGGTCAACCCGGCCGATATCCGCATCGACACC
TACCGTTCGTCAGGCGCGGGCGGTCAGCACGTCAACACCACCGACTCGGCGGTGCGGATCACTCACGAACCCACGGGCAT
TGTAGTGACCTCCTCGGAGAAATCGCAGCACCAGAACCGCGACATCGCCATGAAGGCGCTGAAATCGCGGCTCTACCAGC
TCGAACTTGATCGCCGCAACGCCGAAGTAAACGCGCTGCACGAGGCAAAGGGCGATGCGGGGTGGGGTAACCAGATCCGA
TCCTATGTGATGCAGCCCTACCAGATGGTCAAAGACCTGCGCACCAACCATGAGACCGCCGACACCAAAGGCGTGCTGGA
TGGCGACCTTGATGGGTTCATGGCGGCGACGCTGGCGATGAAGGTTGCCGGCAAAAGCCGCGCCGACGCGCAGGGCGAGG
ATTAA

Upstream 100 bases:

>100_bases
TTGAGGCGGGCAGGCGGGTGATCCTCGCTTGCCCGCGCTCTTGTAGTGGTCCCGGACGTGCGGTATCACCCATCTTCAAA
CCAGACTTCAAGGAATGCCC

Downstream 100 bases:

>100_bases
GCTCCGCGTCCGGCAGTCGCCACAAAGATCATTCGTTGAACAAGGCCCCTGACCGTTTGGTCAGGGGTTTTTTGTCTGGG
ATTGGGGGCGGGTTTTGGAC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRAEIQTIVADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQSLVDAMDTYKSIRQDLQDSVE
LIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGETDPNDAFLEIKAGAGGTEACDWASMLARMYVRWAESRGFS
VELQEEQAGAEAGIKSATYQIKGHNAYGWLKSESGTHRLVRISPFGKGTRETSFAAVGAYPVIDDNIEIEVNPADIRIDT
YRSSGAGGQHVNTTDSAVRITHEPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAEVNALHEAKGDAGWGNQIR
SYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAMKVAGKSRADAQGED

Sequences:

>Translated_374_residues
MRAEIQTIVADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQSLVDAMDTYKSIRQDLQDSVE
LIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGETDPNDAFLEIKAGAGGTEACDWASMLARMYVRWAESRGFS
VELQEEQAGAEAGIKSATYQIKGHNAYGWLKSESGTHRLVRISPFGKGTRETSFAAVGAYPVIDDNIEIEVNPADIRIDT
YRSSGAGGQHVNTTDSAVRITHEPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAEVNALHEAKGDAGWGNQIR
SYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAMKVAGKSRADAQGED
>Mature_374_residues
MRAEIQTIVADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQSLVDAMDTYKSIRQDLQDSVE
LIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGETDPNDAFLEIKAGAGGTEACDWASMLARMYVRWAESRGFS
VELQEEQAGAEAGIKSATYQIKGHNAYGWLKSESGTHRLVRISPFGKGTRETSFAAVGAYPVIDDNIEIEVNPADIRIDT
YRSSGAGGQHVNTTDSAVRITHEPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRNAEVNALHEAKGDAGWGNQIR
SYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAMKVAGKSRADAQGED

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=37.2759856630824, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI34577120, Length=236, Percent_Identity=36.4406779661017, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=39.3782383419689, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI2367172, Length=352, Percent_Identity=51.9886363636364, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1787462, Length=331, Percent_Identity=38.368580060423, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=200, Percent_Identity=30, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321295, Length=237, Percent_Identity=36.2869198312236, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921226, Length=270, Percent_Identity=38.1481481481481, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_SILST (Q1GIB5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612863.1
- ProteinModelPortal:   Q1GIB5
- SMR:   Q1GIB5
- STRING:   Q1GIB5
- GeneID:   4076238
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0868
- NMPDR:   fig|292414.1.peg.2952
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   Q1GIB5
- ProtClustDB:   PRK00578
- BioCyc:   SSP292414:TM1040_0868-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41458; Mature: 41458

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEIQTIVADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQS
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LVDAMDTYKSIRQDLQDSVELIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
TDPNDAFLEIKAGAGGTEACDWASMLARMYVRWAESRGFSVELQEEQAGAEAGIKSATYQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHCCCCCCEEE
IKGHNAYGWLKSESGTHRLVRISPFGKGTRETSFAAVGAYPVIDDNIEIEVNPADIRIDT
EECCCEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCEEEEECCCEEEEEE
YRSSGAGGQHVNTTDSAVRITHEPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRN
CCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCC
AEVNALHEAKGDAGWGNQIRSYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAM
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
KVAGKSRADAQGED
HHCCCCCCCCCCCC
>Mature Secondary Structure
MRAEIQTIVADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQS
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LVDAMDTYKSIRQDLQDSVELIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
TDPNDAFLEIKAGAGGTEACDWASMLARMYVRWAESRGFSVELQEEQAGAEAGIKSATYQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHCCCCCCEEE
IKGHNAYGWLKSESGTHRLVRISPFGKGTRETSFAAVGAYPVIDDNIEIEVNPADIRIDT
EECCCEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCEEEEECCCEEEEEE
YRSSGAGGQHVNTTDSAVRITHEPTGIVVTSSEKSQHQNRDIAMKALKSRLYQLELDRRN
CCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCC
AEVNALHEAKGDAGWGNQIRSYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAM
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
KVAGKSRADAQGED
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA