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The map label for this gene is amiC [H]

Identifier: 99080707

GI number: 99080707

Start: 920518

End: 921756

Strand: Direct

Name: amiC [H]

Synonym: TM1040_0866

Alternate gene names: 99080707

Gene position: 920518-921756 (Clockwise)

Preceding gene: 99080706

Following gene: 99080708

Centisome position: 28.76

GC content: 63.92

Gene sequence:

>1239_bases
ATGCGCGCGCTTTTTGCAAAGCTCTGGCTGTGCCTTACGCTGGTCCATCTGGCGTTGCTGCCTGCTGCCGCCGCGGCGCA
GGGGTTTTCGGCGCTCGCGCGGGTTTTGCCCGAAGACAGCAGCCTTTGGGACACTGGCGCAGGCGTGGAGCTCGAGCTGG
GTCTGAGCCAGGGCGTGCCCTACCGGATCTTTGCGCTCGATGATCCCTATCGTCTGGTGCTCGACTTTCAGGAGGTGGAC
TGGACCGGGCTTCAGGCCGAGGATTTTGACCGCTCCGAGCTGATCGAACGGACACGTTTTGGGGGCTATGTGCCGGGGTG
GTCGCGGATGGTTCTCACCCTCACAGAGCCGATGCAATTGCGCACCGCAGCGATGGACATTGACCCTGAGACCAGCGCGG
CGAAGCTGAAACTTGCCCTTGCCCCCATTGATGCCGAGACATTTGCGGCGACTGCTGGGGCGCCCTACGATCCGCGTCTT
GATCTTCCGATGCCGGCCGAGCTCAAGCCGCGTGCCCCGCGCGATGACGATGCGCCGCTTGTTGTGATGATCGACCCCGG
GCATGGCGGGCTCGACCCCGGAGCCGAGGCCGAAGGCGGGATCGCCGAAAAGGACCTGATGCTGGCCTTTGCCTACGATC
TCGGCGAACGGCTGGTGCGCTCAGGGGCCTTTGAGGTGCTACTGACGCGAGAGGGAGACTACTTTGTCTCTCTCGAGCGC
CGCATTGCCATGGCGCATCAGCAGGGGGCTGATGTGTTCATTTCGCTTCATGCCGACGCGGTCACAGAAGGCATGGCCCA
TGGCACCGTGGTCTACACACTGTCTGAATCTGCCTCTGATGTGGCCTCGGCCAAGCTCGCCGAACGTCACGACCGGGCCG
ACCTGCTGTCTGGCAGTGACCTCAGCGCTGCTGATGATGTGGTTACGGATGTGATGCTGGATCTCGCCCGGCAGGAGACC
CACCCGCGCAGCACTGCTTTGGCGCGCTCGGTGATCGAGGCGCTCATGGAGGCGGGAGGGCCGGTCAATCGCCGCCCGCT
GCGCTCGGCGGGGTTTTCGGTGCTGAAATCCGCTGACATCCCGTCGATCCTGATCGAGCTGGGCTTCATGTCGAGCCCAA
GGGATCTTGAGCTGCTCACGGATCCCGACTGGCAGGCCAAAACCGCGCAAAGCATCCTGAACGGGCTGCTGGTTTGGCGG
GAAGAGGACGCCGCGCGGCGCGCATTGGTGCGCCAATAG

Upstream 100 bases:

>100_bases
TGCGCGGGGCCACCACGTTGCGATTTTCCTATGCCCGCTCCACCGCCGACATCGAGGAGGGGCTGGCGCGCCTCAGGGCC
TTTATGGAGGCGCGGGGCTG

Downstream 100 bases:

>100_bases
CGCCCATACCGCCAATAGCGGCAGTCGAACAGCAAACGGCGGGCTAAGCTTGCGCATGAGGCCCCCTGTGAAAAGGGAAG
TGCTGCGCCGCGCCAGCCCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MRALFAKLWLCLTLVHLALLPAAAAAQGFSALARVLPEDSSLWDTGAGVELELGLSQGVPYRIFALDDPYRLVLDFQEVD
WTGLQAEDFDRSELIERTRFGGYVPGWSRMVLTLTEPMQLRTAAMDIDPETSAAKLKLALAPIDAETFAATAGAPYDPRL
DLPMPAELKPRAPRDDDAPLVVMIDPGHGGLDPGAEAEGGIAEKDLMLAFAYDLGERLVRSGAFEVLLTREGDYFVSLER
RIAMAHQQGADVFISLHADAVTEGMAHGTVVYTLSESASDVASAKLAERHDRADLLSGSDLSAADDVVTDVMLDLARQET
HPRSTALARSVIEALMEAGGPVNRRPLRSAGFSVLKSADIPSILIELGFMSSPRDLELLTDPDWQAKTAQSILNGLLVWR
EEDAARRALVRQ

Sequences:

>Translated_412_residues
MRALFAKLWLCLTLVHLALLPAAAAAQGFSALARVLPEDSSLWDTGAGVELELGLSQGVPYRIFALDDPYRLVLDFQEVD
WTGLQAEDFDRSELIERTRFGGYVPGWSRMVLTLTEPMQLRTAAMDIDPETSAAKLKLALAPIDAETFAATAGAPYDPRL
DLPMPAELKPRAPRDDDAPLVVMIDPGHGGLDPGAEAEGGIAEKDLMLAFAYDLGERLVRSGAFEVLLTREGDYFVSLER
RIAMAHQQGADVFISLHADAVTEGMAHGTVVYTLSESASDVASAKLAERHDRADLLSGSDLSAADDVVTDVMLDLARQET
HPRSTALARSVIEALMEAGGPVNRRPLRSAGFSVLKSADIPSILIELGFMSSPRDLELLTDPDWQAKTAQSILNGLLVWR
EEDAARRALVRQ
>Mature_412_residues
MRALFAKLWLCLTLVHLALLPAAAAAQGFSALARVLPEDSSLWDTGAGVELELGLSQGVPYRIFALDDPYRLVLDFQEVD
WTGLQAEDFDRSELIERTRFGGYVPGWSRMVLTLTEPMQLRTAAMDIDPETSAAKLKLALAPIDAETFAATAGAPYDPRL
DLPMPAELKPRAPRDDDAPLVVMIDPGHGGLDPGAEAEGGIAEKDLMLAFAYDLGERLVRSGAFEVLLTREGDYFVSLER
RIAMAHQQGADVFISLHADAVTEGMAHGTVVYTLSESASDVASAKLAERHDRADLLSGSDLSAADDVVTDVMLDLARQET
HPRSTALARSVIEALMEAGGPVNRRPLRSAGFSVLKSADIPSILIELGFMSSPRDLELLTDPDWQAKTAQSILNGLLVWR
EEDAARRALVRQ

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=396, Percent_Identity=30.8080808080808, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=35.3191489361702, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=35.9307359307359, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44686; Mature: 44686

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALFAKLWLCLTLVHLALLPAAAAAQGFSALARVLPEDSSLWDTGAGVELELGLSQGVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
YRIFALDDPYRLVLDFQEVDWTGLQAEDFDRSELIERTRFGGYVPGWSRMVLTLTEPMQL
EEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHH
RTAAMDIDPETSAAKLKLALAPIDAETFAATAGAPYDPRLDLPMPAELKPRAPRDDDAPL
HHHHCCCCCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VVMIDPGHGGLDPGAEAEGGIAEKDLMLAFAYDLGERLVRSGAFEVLLTREGDYFVSLER
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHH
RIAMAHQQGADVFISLHADAVTEGMAHGTVVYTLSESASDVASAKLAERHDRADLLSGSD
HHHHHHCCCCEEEEEEEHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHCCCCC
LSAADDVVTDVMLDLARQETHPRSTALARSVIEALMEAGGPVNRRPLRSAGFSVLKSADI
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
PSILIELGFMSSPRDLELLTDPDWQAKTAQSILNGLLVWREEDAARRALVRQ
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHCC
>Mature Secondary Structure
MRALFAKLWLCLTLVHLALLPAAAAAQGFSALARVLPEDSSLWDTGAGVELELGLSQGVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
YRIFALDDPYRLVLDFQEVDWTGLQAEDFDRSELIERTRFGGYVPGWSRMVLTLTEPMQL
EEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHH
RTAAMDIDPETSAAKLKLALAPIDAETFAATAGAPYDPRLDLPMPAELKPRAPRDDDAPL
HHHHCCCCCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VVMIDPGHGGLDPGAEAEGGIAEKDLMLAFAYDLGERLVRSGAFEVLLTREGDYFVSLER
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHH
RIAMAHQQGADVFISLHADAVTEGMAHGTVVYTLSESASDVASAKLAERHDRADLLSGSD
HHHHHHCCCCEEEEEEEHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHCCCCC
LSAADDVVTDVMLDLARQETHPRSTALARSVIEALMEAGGPVNRRPLRSAGFSVLKSADI
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
PSILIELGFMSSPRDLELLTDPDWQAKTAQSILNGLLVWREEDAARRALVRQ
HHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]