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The map label for this gene is potI [H]

Identifier: 99080677

GI number: 99080677

Start: 880854

End: 881654

Strand: Reverse

Name: potI [H]

Synonym: TM1040_0836

Alternate gene names: 99080677

Gene position: 881654-880854 (Counterclockwise)

Preceding gene: 99080678

Following gene: 99080676

Centisome position: 27.54

GC content: 57.05

Gene sequence:

>801_bases
ATGTCTAAAGGATTTGATGTTCGCCGCCAGACGGGCTTCACAGCCATCGCCATCGCCTGCTTTGTGCTGCTGTACCTGCC
GATTCTACTTCTAGTCGCCTATTCCTTTAACGCTGGCGACTCCATCGCAATCTGGGAAGGGTTTTCCTGGCGCTGGTACG
CGTCGGCCTGGGATAACGAACAGGTCAAGGATGCCACGATCCGCTCTCTGATCCTTGCGACCTGCGCGTCGGGTGTCGCA
ACCACCGCAGCGGTTCTAGCGGCACTGGCCACCACCCGCAGCAAGCCCTGGCGCGGGCAGACCGTGGTCTTTGCAATGGT
CAATCAACCGTTGATGGTGCCAGAGATCGTCACCGCGGTCTCGCTCCTCATTGTGTTTGCCAGCATCAAATCCTTTACCG
GCTATACCGGGCTTGGCTACCTGATCCTCGCACATTCGGCCTTTTGCATTCCCTTTGCCTATATGCCGGTGCGTGCGCGA
TTGCAGGGCATGGATCTGAGCCTCGAACACGCGGGCGCTGATCTGTATGGCACACCGTTTCAGGTCTTTCGCCACATCAC
CCTGCCCCAGCTCTGGCCTGGAATTCTCGCCGGAGCGATGCTGGCCTTCGTGATCTCTCTTGATGACGTGGTGATCACCG
AGTTCGTCAAATCCGCCGGACAGGACACCTTGCCCACATATATGCTGGGACAATTGCGCCGTGTGGTAACGCCCGAGATC
AACGCCATTTCCACCGCCCTATTGGCGATCTCGGTCCTGATGGTGACCGCTTTTTACTTTGTGAACAAAGTCCGAAAATA
A

Upstream 100 bases:

>100_bases
AACTGGCCCCTTGGCGCCGCGCTGGCGCTGACGCTTTTGGCGGTGGTGATGGTGGCGCTGGTGTTCTACGTGCGCGCCAT
CGGGCGGGAGGGCGAAAGCG

Downstream 100 bases:

>100_bases
CTCCAAAACCATGAGCCAGAGACAACTGGCCGCCCATAAACCCAACATCCGACAGGAGAAATGACTTATGAGACATCTGA
TTGCAGCCACAACGCTTGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSKGFDVRRQTGFTAIAIACFVLLYLPILLLVAYSFNAGDSIAIWEGFSWRWYASAWDNEQVKDATIRSLILATCASGVA
TTAAVLAALATTRSKPWRGQTVVFAMVNQPLMVPEIVTAVSLLIVFASIKSFTGYTGLGYLILAHSAFCIPFAYMPVRAR
LQGMDLSLEHAGADLYGTPFQVFRHITLPQLWPGILAGAMLAFVISLDDVVITEFVKSAGQDTLPTYMLGQLRRVVTPEI
NAISTALLAISVLMVTAFYFVNKVRK

Sequences:

>Translated_266_residues
MSKGFDVRRQTGFTAIAIACFVLLYLPILLLVAYSFNAGDSIAIWEGFSWRWYASAWDNEQVKDATIRSLILATCASGVA
TTAAVLAALATTRSKPWRGQTVVFAMVNQPLMVPEIVTAVSLLIVFASIKSFTGYTGLGYLILAHSAFCIPFAYMPVRAR
LQGMDLSLEHAGADLYGTPFQVFRHITLPQLWPGILAGAMLAFVISLDDVVITEFVKSAGQDTLPTYMLGQLRRVVTPEI
NAISTALLAISVLMVTAFYFVNKVRK
>Mature_265_residues
SKGFDVRRQTGFTAIAIACFVLLYLPILLLVAYSFNAGDSIAIWEGFSWRWYASAWDNEQVKDATIRSLILATCASGVAT
TAAVLAALATTRSKPWRGQTVVFAMVNQPLMVPEIVTAVSLLIVFASIKSFTGYTGLGYLILAHSAFCIPFAYMPVRARL
QGMDLSLEHAGADLYGTPFQVFRHITLPQLWPGILAGAMLAFVISLDDVVITEFVKSAGQDTLPTYMLGQLRRVVTPEIN
AISTALLAISVLMVTAFYFVNKVRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=255, Percent_Identity=36.4705882352941, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787368, Length=223, Percent_Identity=34.9775784753363, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787714, Length=246, Percent_Identity=31.3008130081301, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29072; Mature: 28940

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGFDVRRQTGFTAIAIACFVLLYLPILLLVAYSFNAGDSIAIWEGFSWRWYASAWDNE
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCC
QVKDATIRSLILATCASGVATTAAVLAALATTRSKPWRGQTVVFAMVNQPLMVPEIVTAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHH
SLLIVFASIKSFTGYTGLGYLILAHSAFCIPFAYMPVRARLQGMDLSLEHAGADLYGTPF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCHH
QVFRHITLPQLWPGILAGAMLAFVISLDDVVITEFVKSAGQDTLPTYMLGQLRRVVTPEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
NAISTALLAISVLMVTAFYFVNKVRK
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKGFDVRRQTGFTAIAIACFVLLYLPILLLVAYSFNAGDSIAIWEGFSWRWYASAWDNE
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCC
QVKDATIRSLILATCASGVATTAAVLAALATTRSKPWRGQTVVFAMVNQPLMVPEIVTAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHH
SLLIVFASIKSFTGYTGLGYLILAHSAFCIPFAYMPVRARLQGMDLSLEHAGADLYGTPF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCHH
QVFRHITLPQLWPGILAGAMLAFVISLDDVVITEFVKSAGQDTLPTYMLGQLRRVVTPEI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
NAISTALLAISVLMVTAFYFVNKVRK
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]