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The map label for this gene is potD-A [H]

Identifier: 99080676

GI number: 99080676

Start: 879761

End: 880786

Strand: Reverse

Name: potD-A [H]

Synonym: TM1040_0835

Alternate gene names: 99080676

Gene position: 880786-879761 (Counterclockwise)

Preceding gene: 99080677

Following gene: 99080675

Centisome position: 27.52

GC content: 56.24

Gene sequence:

>1026_bases
ATGAGACATCTGATTGCAGCCACAACGCTTGCGACCGTCTCCGCGACGGCGGCCATGGCGGAGGGGCAGTTGAATATCTA
CAACTGGGGCAACTACACCAGCCCGGAATTGATCGAGAAGTTCGAACAGGAGTTCGACATCGACGTCACCATCACCGACT
ATGACAGCAACGACACCGCACTGGCCAAGATCAAGGCAGGTGGGCATGGCTTTGACATCGTGGTGCCGTCTGGCACCTAT
GTGCCGATCTTCATCGGCGAAGGCCTGTTGATGAAGTCGATGCCCAACCAGATGGAGAACTTCAAGAACATGGACCCGCG
CTGGGTCGATGTGGATTTTGATCCTGGCCGCGACTACACCGTGCCTTGGCAATGGGGCACCGTGGGCGTCACCGTCAATA
CTTCGGTTTATTCAGGCGACATCAACTCGGCGGCACTGATCTTTGATCCGCCAGAAGAGCTGAAGGGCAAGATCAACGTC
GTTCCAGAGATGCTCGACGTGATGGGCATGGCCATTCACTACATGGGCGGAGAGCAATGCACCGCCGACAAGGACATGCT
GGCCAAAGTGCGCGATAAACTGGTCGAGGCCAAAAAGGACTGGCTCTCCATGGCCTATGGCAACATCGAGAAGTTCGCCA
AGGGCGACCTCGCGGCTGGGGTCAATTGGAACGGCGCCTCATTCCGGGCACGTCTGCAAAACGATGACATCGCCTTTGGC
TATCCACAGACCGGGTTTTCGATCTGGATGGACAACGCCGCGATCCTCGCGGATGCGCAGAATGTCGACAATGCCAAACT
GTTCCTGAACTATATCATGGCTCCGGAGAACGCAGCGCTTCTGTCCAATTTTGCCCGCTACGCCAATGGCATCAAAGGAT
CTGAACCCTTTATGGATGCGGCCATGGCAGAGGCCTCCGAGGTGGTTATTCCCGACGAGCTCAAAGATGCCGGCTATCTC
GCCAAGACCTGCCCACCCGACGTGCAGCGGATCTATTCCAAGATCTGGACCGAAGTGACCAAATAA

Upstream 100 bases:

>100_bases
GCTTTTTACTTTGTGAACAAAGTCCGAAAATAACTCCAAAACCATGAGCCAGAGACAACTGGCCGCCCATAAACCCAACA
TCCGACAGGAGAAATGACTT

Downstream 100 bases:

>100_bases
CCCCGCAGAAGGGGCGTAACAGCCCCGGGGCGCGCCAAATCGGGGCGCGCCCCCACCCCAAAACTGGACTCCGCGTGATG
ACCATCCCTCCTCAGACCGT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MRHLIAATTLATVSATAAMAEGQLNIYNWGNYTSPELIEKFEQEFDIDVTITDYDSNDTALAKIKAGGHGFDIVVPSGTY
VPIFIGEGLLMKSMPNQMENFKNMDPRWVDVDFDPGRDYTVPWQWGTVGVTVNTSVYSGDINSAALIFDPPEELKGKINV
VPEMLDVMGMAIHYMGGEQCTADKDMLAKVRDKLVEAKKDWLSMAYGNIEKFAKGDLAAGVNWNGASFRARLQNDDIAFG
YPQTGFSIWMDNAAILADAQNVDNAKLFLNYIMAPENAALLSNFARYANGIKGSEPFMDAAMAEASEVVIPDELKDAGYL
AKTCPPDVQRIYSKIWTEVTK

Sequences:

>Translated_341_residues
MRHLIAATTLATVSATAAMAEGQLNIYNWGNYTSPELIEKFEQEFDIDVTITDYDSNDTALAKIKAGGHGFDIVVPSGTY
VPIFIGEGLLMKSMPNQMENFKNMDPRWVDVDFDPGRDYTVPWQWGTVGVTVNTSVYSGDINSAALIFDPPEELKGKINV
VPEMLDVMGMAIHYMGGEQCTADKDMLAKVRDKLVEAKKDWLSMAYGNIEKFAKGDLAAGVNWNGASFRARLQNDDIAFG
YPQTGFSIWMDNAAILADAQNVDNAKLFLNYIMAPENAALLSNFARYANGIKGSEPFMDAAMAEASEVVIPDELKDAGYL
AKTCPPDVQRIYSKIWTEVTK
>Mature_341_residues
MRHLIAATTLATVSATAAMAEGQLNIYNWGNYTSPELIEKFEQEFDIDVTITDYDSNDTALAKIKAGGHGFDIVVPSGTY
VPIFIGEGLLMKSMPNQMENFKNMDPRWVDVDFDPGRDYTVPWQWGTVGVTVNTSVYSGDINSAALIFDPPEELKGKINV
VPEMLDVMGMAIHYMGGEQCTADKDMLAKVRDKLVEAKKDWLSMAYGNIEKFAKGDLAAGVNWNGASFRARLQNDDIAFG
YPQTGFSIWMDNAAILADAQNVDNAKLFLNYIMAPENAALLSNFARYANGIKGSEPFMDAAMAEASEVVIPDELKDAGYL
AKTCPPDVQRIYSKIWTEVTK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=360, Percent_Identity=27.7777777777778, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1787367, Length=269, Percent_Identity=31.9702602230483, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37582; Mature: 37582

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLIAATTLATVSATAAMAEGQLNIYNWGNYTSPELIEKFEQEFDIDVTITDYDSNDTA
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCE
LAKIKAGGHGFDIVVPSGTYVPIFIGEGLLMKSMPNQMENFKNMDPRWVDVDFDPGRDYT
EEEEECCCCEEEEEECCCCEEEEEECCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCCEE
VPWQWGTVGVTVNTSVYSGDINSAALIFDPPEELKGKINVVPEMLDVMGMAIHYMGGEQC
ECEECCEEEEEEECEEEECCCCCEEEEECCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCC
TADKDMLAKVRDKLVEAKKDWLSMAYGNIEKFAKGDLAAGVNWNGASFRARLQNDDIAFG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCEEEEECCCCEEEC
YPQTGFSIWMDNAAILADAQNVDNAKLFLNYIMAPENAALLSNFARYANGIKGSEPFMDA
CCCCCCEEEECCEEEEEECCCCCCCEEEEEEHCCCCCHHHHHHHHHHHCCCCCCCCHHHH
AMAEASEVVIPDELKDAGYLAKTCPPDVQRIYSKIWTEVTK
HHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHLIAATTLATVSATAAMAEGQLNIYNWGNYTSPELIEKFEQEFDIDVTITDYDSNDTA
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCE
LAKIKAGGHGFDIVVPSGTYVPIFIGEGLLMKSMPNQMENFKNMDPRWVDVDFDPGRDYT
EEEEECCCCEEEEEECCCCEEEEEECCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCCEE
VPWQWGTVGVTVNTSVYSGDINSAALIFDPPEELKGKINVVPEMLDVMGMAIHYMGGEQC
ECEECCEEEEEEECEEEECCCCCEEEEECCHHHHCCEEEEHHHHHHHHHHHHHCCCCCCC
TADKDMLAKVRDKLVEAKKDWLSMAYGNIEKFAKGDLAAGVNWNGASFRARLQNDDIAFG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCEEEEECCCCEEEC
YPQTGFSIWMDNAAILADAQNVDNAKLFLNYIMAPENAALLSNFARYANGIKGSEPFMDA
CCCCCCEEEECCEEEEEECCCCCCCEEEEEEHCCCCCHHHHHHHHHHHCCCCCCCCHHHH
AMAEASEVVIPDELKDAGYLAKTCPPDVQRIYSKIWTEVTK
HHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]