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The map label for this gene is potB [H]

Identifier: 99080678

GI number: 99080678

Start: 881647

End: 882579

Strand: Reverse

Name: potB [H]

Synonym: TM1040_0837

Alternate gene names: 99080678

Gene position: 882579-881647 (Counterclockwise)

Preceding gene: 99080679

Following gene: 99080677

Centisome position: 27.57

GC content: 60.13

Gene sequence:

>933_bases
ATGCAGGATCCCGCTCTCACTGCCCCGCCCCACCCCGATCCGCGCGCCCGGCGCGAGCGGCGCAGCCGGTGGTTGCTGCT
CACACCTGCACTTGTAATCCTCAGCCTTGCCGCAAGTGGGCCATTGCTGGTGGTGGTGATCTACTCATTCCTCACCCCCG
GTGATTACGGCGGTGTTGAATGGCGCTTTACGTTGGAGCCCTGGTTCAACGTGGTGGCCGAGCGGGACTTTTTCACCGAA
GAGCTGCAACTGGCGGACGCGCATCTTCTGGTGTTCTGGCGCTCTATCAAGCTCTCGCTGATGACGGCGTTTCTCTGTCT
GATCGTGGGCTTTCCCACCGCCTATTTCATCGCCACGCGCCCTCAGCACCGCCGGGACATGTGGATGCTGCTGATCATGA
TCCCTTTCTGGACCAATCTGTTGATCCGAACCTTTGCGATCATGGAGCTCATTCGCAACCAAGGCACCATCAACACGCTG
TTGATGGCCAGCGGGCTCATTGATGAGCCGATCCAGATGCTGTTCACAGAGTTTTCGGTTCTGATCGGGATGACCTATGT
CTATCTGCCGCTGATGATCCTGCCCATCTATGCCTCGATGGAGCGGCTCGGTTTCGATTTGGTGGAGGCGGGCTATGACC
TCTATGCCAGCCGACTGGACGTACTGCGGCGCATCATCTTCCCCTTGGTGAAACCTGGCGTTGTGGCTGGCTCCATTCTT
GTCTTTGTGCCCTGCCTTGGCGCCTATGTCACCCCACGCGTGCTGGGCGGCGGCAAGCAGCTGATGCTGGGCAACCTCAT
TGAGCTGCAGTTTGGGCAGTTGCGCAACTGGCCCCTTGGCGCCGCGCTGGCGCTGACGCTTTTGGCGGTGGTGATGGTGG
CGCTGGTGTTCTACGTGCGCGCCATCGGGCGGGAGGGCGAAAGCGATGTCTAA

Upstream 100 bases:

>100_bases
CGGCAAAACCAGCCGGGACAAACGCAGACCTTGGCGATCAATGACACGGTGGGGGTGTGCTTTGCGCCTGACACCGCGCA
GATCCTGAAGGATTGAGCCC

Downstream 100 bases:

>100_bases
AGGATTTGATGTTCGCCGCCAGACGGGCTTCACAGCCATCGCCATCGCCTGCTTTGTGCTGCTGTACCTGCCGATTCTAC
TTCTAGTCGCCTATTCCTTT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQDPALTAPPHPDPRARRERRSRWLLLTPALVILSLAASGPLLVVVIYSFLTPGDYGGVEWRFTLEPWFNVVAERDFFTE
ELQLADAHLLVFWRSIKLSLMTAFLCLIVGFPTAYFIATRPQHRRDMWMLLIMIPFWTNLLIRTFAIMELIRNQGTINTL
LMASGLIDEPIQMLFTEFSVLIGMTYVYLPLMILPIYASMERLGFDLVEAGYDLYASRLDVLRRIIFPLVKPGVVAGSIL
VFVPCLGAYVTPRVLGGGKQLMLGNLIELQFGQLRNWPLGAALALTLLAVVMVALVFYVRAIGREGESDV

Sequences:

>Translated_310_residues
MQDPALTAPPHPDPRARRERRSRWLLLTPALVILSLAASGPLLVVVIYSFLTPGDYGGVEWRFTLEPWFNVVAERDFFTE
ELQLADAHLLVFWRSIKLSLMTAFLCLIVGFPTAYFIATRPQHRRDMWMLLIMIPFWTNLLIRTFAIMELIRNQGTINTL
LMASGLIDEPIQMLFTEFSVLIGMTYVYLPLMILPIYASMERLGFDLVEAGYDLYASRLDVLRRIIFPLVKPGVVAGSIL
VFVPCLGAYVTPRVLGGGKQLMLGNLIELQFGQLRNWPLGAALALTLLAVVMVALVFYVRAIGREGESDV
>Mature_310_residues
MQDPALTAPPHPDPRARRERRSRWLLLTPALVILSLAASGPLLVVVIYSFLTPGDYGGVEWRFTLEPWFNVVAERDFFTE
ELQLADAHLLVFWRSIKLSLMTAFLCLIVGFPTAYFIATRPQHRRDMWMLLIMIPFWTNLLIRTFAIMELIRNQGTINTL
LMASGLIDEPIQMLFTEFSVLIGMTYVYLPLMILPIYASMERLGFDLVEAGYDLYASRLDVLRRIIFPLVKPGVVAGSIL
VFVPCLGAYVTPRVLGGGKQLMLGNLIELQFGQLRNWPLGAALALTLLAVVMVALVFYVRAIGREGESDV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=261, Percent_Identity=38.3141762452107, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=36.3636363636364, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787713, Length=252, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34948; Mature: 34948

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDPALTAPPHPDPRARRERRSRWLLLTPALVILSLAASGPLLVVVIYSFLTPGDYGGVE
CCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEE
WRFTLEPWFNVVAERDFFTEELQLADAHLLVFWRSIKLSLMTAFLCLIVGFPTAYFIATR
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECC
PQHRRDMWMLLIMIPFWTNLLIRTFAIMELIRNQGTINTLLMASGLIDEPIQMLFTEFSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
LIGMTYVYLPLMILPIYASMERLGFDLVEAGYDLYASRLDVLRRIIFPLVKPGVVAGSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VFVPCLGAYVTPRVLGGGKQLMLGNLIELQFGQLRNWPLGAALALTLLAVVMVALVFYVR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIGREGESDV
HHCCCCCCCC
>Mature Secondary Structure
MQDPALTAPPHPDPRARRERRSRWLLLTPALVILSLAASGPLLVVVIYSFLTPGDYGGVE
CCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEE
WRFTLEPWFNVVAERDFFTEELQLADAHLLVFWRSIKLSLMTAFLCLIVGFPTAYFIATR
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECC
PQHRRDMWMLLIMIPFWTNLLIRTFAIMELIRNQGTINTLLMASGLIDEPIQMLFTEFSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
LIGMTYVYLPLMILPIYASMERLGFDLVEAGYDLYASRLDVLRRIIFPLVKPGVVAGSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VFVPCLGAYVTPRVLGGGKQLMLGNLIELQFGQLRNWPLGAALALTLLAVVMVALVFYVR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AIGREGESDV
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]